LOCUS       sequence-336854-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 L85R mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_L85R_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(120..140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        125..454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      305..440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        502..549
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             568..942
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1109..1128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1163..1182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1242..1258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1242..1258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1255..1277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1266..1282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1290..1296,1297..1314,1315..1320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1335..1356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3443..3462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3634..4013
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4015..4213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4168..4188
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4231..4411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4458..4583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5076..5309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5494..5538
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5836..5953
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6089..7267
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6320..7258
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7215..7235
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7284..7303
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7284..7302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7366..8388
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7366..8388
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8398..8454
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(10008..10041)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10066..10246
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10272..10289)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10278..10502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10548..10865,1..111)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10635..10654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(10845..10865,1)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
       61 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
      121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      361 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      421 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
      481 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
      541 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
      601 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
      661 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
      721 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
      781 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
      841 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
      901 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
      961 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     1021 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     1081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     1201 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     3421 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     3481 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     3541 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     3601 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     3661 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3721 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3781 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3841 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3901 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3961 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     4021 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     4081 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     4141 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     4201 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     4261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4381 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     4441 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     4501 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     4561 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4621 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     4681 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     4741 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     4801 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     4861 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     4921 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     4981 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     5041 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     5101 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     5161 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     5221 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     5281 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     5341 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     5401 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     5461 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     5521 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     5581 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     5641 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     5701 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     5761 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     5821 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     5881 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     5941 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaatcg
     6001 ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     6061 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     6121 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     6181 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     6241 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     6301 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     6361 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     6421 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     6481 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     6541 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     6601 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     6661 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     6721 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     6781 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     6841 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     6901 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     6961 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     7021 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     7081 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     7141 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     7201 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     7261 gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca agctggctag
     7321 gtaagcttga tatcgaattc ctgcagcccg ggggatcccg ccaccatgaa aaagcctgaa
     7381 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     7441 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     7501 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     7561 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     7621 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     7681 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     7741 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     7801 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     7861 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     7921 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     7981 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     8041 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     8101 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     8161 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     8221 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     8281 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     8341 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaagg atccggcgca
     8401 acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg accgatgcgt
     8461 atccccgtag atgccagcac gagccgccgc ttcacgccgc cttccaccgc gctgagccca
     8521 ggcaagatga gcgaggcgtt gccgctgggc gccccggacg ccggcgctgc cctggccggc
     8581 aagctgagga gcggcgaccg cagcatggtg gaggtgctgg ccgaccaccc gggcgagctg
     8641 gtgcgcaccg acagccccaa cttcctctgc tccgtgctgc ctacgcactg gcgctgcaac
     8701 aagacccggc ccatcgcttt caaggtggtg gccctagggg atgttccaga tggcactctg
     8761 gtcactgtga tggctggcaa tgatgaaaac tactcggctg agctgagaaa tgctaccgca
     8821 gccatgaaga accaggttgc aagatttaat gacctcaggt ttgtcggtcg aagtggaaga
     8881 gggaaaagct tcactctgac catcactgtc ttcacaaacc caccgcaagt cgccacctac
     8941 cacagagcca tcaaaatcac agtggatggg ccccgagaac ctcgaagaca tcggcagaaa
     9001 ctagatgatc agaccaagcc cgggagcttg tccttttccg agcggctcag tgaactggag
     9061 cagctgcggc gcacagccat gagggtcagc ccacaccacc cagcccccac gcccaaccct
     9121 cgtgcctccc tgaaccactc cactgccttt aaccctcagc ctcagagtca gatgcaggat
     9181 acaaggcaga tccaaccatc cccaccgtgg tcctacgatc agtcctacca atacctggga
     9241 tccattgcct ctccttctgt gcacccagca acgcccattt cacctggacg tgccagcggc
     9301 atgacaaccc tctctgcaga actttccagt cgactctcaa cggcacccga cctgacagcg
     9361 ttcagcgacc cgcgccagtt ccccgcgctg ccctccatct ccgacccccg catgcactat
     9421 ccaggcgcct tcacctactc cccgacgccg gtcacctcgg gcatcggcat cggcatgtcg
     9481 gccatgggct cggccacgcg ctaccacacc tacctgccgc cgccctaccc cggctcgtcg
     9541 caagcgcagg gaggcccgtt ccaagccagc tcgccctcct accacctgta ctacggcgcc
     9601 tcggccggct cctaccagtt ctccatggtg ggcggcgagc gctcgccgcc gcgcatcctg
     9661 ccgccctgca ccaacgcctc caccggctcc gcgctgctca accccagcct cccgaaccag
     9721 agcgacgtgg tggaggccga gggcagccac agcaactccc ccaccaacat ggcgccctcc
     9781 gcgcgcctgg aggaggccgt gtggaggccc tactgactag ctaagttaga actatttcct
     9841 ggctgttacg cggcgctcgc gtaactactg acgggcttgt taaacagtga accgcccgcc
     9901 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga acaaaagaaa
     9961 aggggggact ggaagggcta attcactccc aacgaagaca agatatcata acttcgtata
    10021 gcatacatta tacgaagtta tcgtctagct ggtccggact gtactgggtc tctctggtta
    10081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
    10261 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    10321 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    10381 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    10441 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    10501 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
    10561 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    10621 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    10681 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10741 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10801 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10861 aactg
//
