LOCUS       sequence-336923-        4345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK246
FEATURES             Location/Qualifiers
     source          1..4345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(316..335)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             327..1142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(400..419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1346..1368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1360..1377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1361..1377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3116..3132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3116..3132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3129..3151)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3140..3156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3164..3170,3171..3188,3189..3194))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3209..3230
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3531..3548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3702..4290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3782..3801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg
      121 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
      181 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
      241 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      301 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
      361 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      421 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      481 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      541 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      601 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
      661 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      721 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      781 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      841 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
      901 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      961 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1021 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1081 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1141 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1201 gcggctttgt tgaataaatc gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     1381 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagga agctgctctt
     1441 cgtggcaggt cgactccacg taatttcaca acatccggga aaagaagtga aaaaaggact
     1501 cgagcaactg tacaagaaaa ttgagaagaa cctggttgcc aattcatcac ttttgcaagt
     1561 tgtatggaga gatatgcaag agcaatttgt caagcaaatt gcggaataca acaaactgat
     1621 tctcacttgt tacccaggct cgaagattga gctcgaagtt tccactgaca ctgtacttca
     1681 gttcttctca gaaattgctc aacaacacta atattacgac gaataatttt attgaatttt
     1741 gttgttttaa ttcatttaaa atttagtttt aatttatcag aactctaata cttactgaac
     1801 catgctttgt aatatattta tttatctatt ctgtaatagg tgaacttcgt tacattactg
     1861 atgtctgtaa tataaattta cgggtgttcc ccattcgaaa atagtagtaa tgcttgcttt
     1921 tttgaaaaat aaatgtcttg cttggtgact tttatttcac gtattttcaa atgctcacaa
     1981 aaatttcacg gaactgactt cttggttaca catttgtaga aattttgact gaatgtttgc
     2041 attgaactgg tcaacttaag aatctgacat gcaaatggtg tgttttctga agaacacact
     2101 ttggcaactt ttaataaaaa aggggtgtaa cacgatttaa atctaataaa aacaacttct
     2161 ataatgttat tccgtttata tctcacttta tgttttacat tttactctta tatacatttt
     2221 ttgactcatt tatacagggt ggtccataat tatggaccac cctgtactta tatcttattt
     2281 aaaactattt catgtaagaa ttgttttaac agcgcatttt ttttcatcac tcgatttaaa
     2341 ggtaaaattg ataggccgca atctattttg gaaaacttca gaacaaaatt taacatttta
     2401 agaggtaact gtgaaggttt ataagagaat tataaaccga tctaaccact aaacatggta
     2461 acgtagaaaa agtaaaatta ggggaggggg gatgtttatt tattcttggt tttcaattct
     2521 tatcagactc tgtaaagtaa aatgaacata acttgttcga atagtgtagg aatttcaaac
     2581 aagctacaaa tctagctcca catacgcgtg gaatagccgg tcataaatcg tcttccgtta
     2641 accgcatggc tagatccaca ccgattccat ttttcttgaa taaaacaata gcttgatgaa
     2701 aattgggtag ccaccgaaaa aactgattcg cgatccggat tattggtgac aaacatacgg
     2761 caagtgaaag tgttgcactg atagaaaccg atacgagaat atgccttttg ggcccccccc
     2821 cccccccccc tacggcttca ctcccggtgt tgtagatctg actttcactt gccttatctc
     2881 gaggaatctg gagaggagaa ggagggacaa ccgatccgcg cgcgcgtaac aatacgggag
     2941 atatagagga aaagcagcaa aaagacattt tgatgacctt attttcttgt cgttgtcgag
     3001 acggccattg tcccttccac gcccagcttt tcattgtagt tcaaatcgct tctcattgac
     3061 aatcaacctg ccaattgttt ctcggaagcg aagagcacct cgaattcgta atcatgtcat
     3121 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3301 aacgcgcggg gagaggcggt ttgcgtattg gcgaactttt gctgagttga aggatcagat
     3361 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcagtaa
     3421 ccgtcagtgc cgataagttc aaagttaaac ctggtgttga taccaacatt gaaacgctga
     3481 tcgaaaacgc gctgaaaaac gctgctgaat gtgcgagctt cttccgcttc ctcgctcact
     3541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     3901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4141 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4321 gacgctcagt ggaacgatcc gtcga
//
