LOCUS       sequence-33693-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J,
            Li L, Zheng P, Ye K, Chinnaiyan A, Halder G, Lai ZC, Guan KL
  TITLE     Inactivation of YAP oncoprotein by the Hippo pathway is involved in 
            cell contact inhibition and tissue growth control.
  JOURNAL   Genes Dev. 2007 Nov 1. 21(21):2747-61.
  PUBMED    17974916
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2007 Nov 1. 21(21):2747-61."
COMMENT     Sequence Label: pCMV-flag Mst2 (KR)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..56
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             725..733
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctcgtttagt gaccgtcaga attgccacca tggactacaa ggacgacgat gacaagaatt
       61 ctgcaggatc catggagcag ccgccggcgc ctaagagtaa actaaaaaag ctgagtgaag
      121 acagtttgac taagcagcct gaagaagttt ttgatgtatt agagaagctt ggagaagggt
      181 cttatggaag tgtatttaaa gcaatacaca aggaatccgg tcaagttgtc gcaattagac
      241 aagtacctgt tgaatcagat cttcaggaaa taatcaaaga aatttccata atgcagcaat
      301 gtgacagccc atatgttgta aagtactatg gcagttattt taagaataca gacctctgga
      361 ttgttatgga gtactgtggc gctggctctg tctcagacat aattagatta cgaaacaaga
      421 cattaataga agatgaaatt gcaaccattc ttaaatctac attgaaagga ctagaatatt
      481 tgcactttat gagaaaaata cacagagata taaaagctgg aaatattctc ctcaatacag
      541 aaggacatgc aaaattggca gattttggag tggctggtca gttaacagat acaatggcaa
      601 aacgcaatac tgtaatagga actccatttt ggatggctcc tgaggtgatt caagaaatag
      661 gctataactg tgtggccgac atctggtccc ttggcattac ttctatagaa atggctgaag
      721 gaaaacctcc ttatgctgat atacatccaa tgagggctat ttttatgatt cccacaaatc
      781 caccaccaac attcagaaag ccagaacttt ggtccgatga tttcaccgat tttgttaaa
//
