LOCUS       sequence-336946-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial coexpression of human CITED2 CTAD with CBP TAZ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Berlow RB, Dyson HJ, Wright PE
  TITLE     Hypersensitive termination of the hypoxic response by a disordered 
            protein switch.
  JOURNAL   Nature. 2017 Mar 16;543(7645):447-451. doi: 10.1038/nature21705. 
            Epub 2017 Mar 8.
  PUBMED    28273070
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21705"; journalName: "Nature"; date: "2017-03-16- 
            16"; volume: "543"; issue: "7645"; pages: "447-451"
COMMENT     Sequence Label: HisGB1-CITED2(216-269)+CBPTAZ1(340-439)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             25..47
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..254
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             279..296
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             493..515
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             852..869
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggcc
       61 atcatcatca tcatcacagc agcggcatga cttacaaact gatccttaac ggtaaaactt
      121 tgaaaggcga aaccactacc gaagctgttg atgctgcgac tgcagaaaaa gttttcaaac
      181 agtacgctaa cgacaacggt gttgacggtg aatggactta cgacgatgcg actaagacct
      241 tcaccgttac tgaagaattc agcagcggca gcagcggcct ggttccgcgt ggttcccata
      301 tgtccaatgt catagacact gatttcatcg acgaggaagt tcttatgtcc ttggtgatag
      361 aaatgggttt ggaccgcatc aaggagctgc ccgaactctg gctggggcaa aacgagtttg
      421 attttatgac ggacttcgtg tgcaaacagc agcccagcag agtgagctaa taggatcccc
      481 tctagaaata attttgttta actttaagaa ggagatatac catggcaaca ggccccacag
      541 cagaccctga aaaacgcaaa ctgatacagc agcagctggt tctactgctt catgcccaca
      601 aatgtcagag acgagagcaa gcaaatggag aggttcgagc ctgttctctc ccacactgtc
      661 gaaccatgaa aaacgttttg aatcacatga cacattgtca ggctgggaaa gcctgccaag
      721 ttgcccattg tgcatcttca cgacaaatca tctctcattg gaagaactgc acacgacatg
      781 actgtcctgt ttgcctccct ttgaaaaatg ccagtgacaa gcgataatag aagcttgcgg
      841 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gc
//
