LOCUS       sequence-336960-        1794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 
            truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Yang Y, Liu J, Clarke BR, Seidel L, Bolla JR, Ward PN, Zhang P, 
            Robinson CV, Whitfield C, Naismith JH
  TITLE     The molecular basis of regulation of bacterial capsule assembly by 
            Wzc.
  JOURNAL   Nat Commun. 2021 Jul 16;12(1):4349. doi: 10.1038/s41467-021-24652-1.
  PUBMED    34272394
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-16"; volume: "12(1):4349. doi"; pages: " 
            10.1038/s41467-021-24652-1"
COMMENT     Sequence Label: pBN093
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1774..1791
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgacttcag tcacatctaa gcaatcaacg atactaggat ctgatgaaat agatttaggt
       61 cgcgttatcg gtgagctaat tgatcatcgt aagcttatta tatctataac atcggttttt
      121 accttatttg caatactcta tgcattatta gcgactccta tctatgagac ggatgcgcta
      181 attcaaatag agcagaagca aggtaatgct atactaagca gtctaagtca ggttttacct
      241 gatggccaac cccaatctgc acctgaaaca gctttgctac aatctcgaat gattcttggc
      301 aaaactatag atgatttaaa ccttcagatt caaattgaac aaaaatattt tcctgttatt
      361 ggccgtgggt tagcaagatt aatgggagaa aaaccaggta atattgatat tactcggtta
      421 tatttgcctg atagtgacga tattagtaat aatactccta gtataatatt aacagtaaaa
      481 gataaagaaa attattcgat caatagcgat ggtattcaac tcaatggggt tgtagggacg
      541 ttattaaatg aaaaagggat atcattactg gttaacgaaa tcgatgctaa acctggagat
      601 caatttgtta ttacacagtt gcctcgatta aaggccattt ctgatctttt aaaaagtttt
      661 agcgttgctg atttgggtaa agatacggga atgttaacgt tgactcttac tggagataac
      721 ccaaaaagaa tatctcatat tttggacagt atcagtcaga actatcttgc acagaacatt
      781 gctagacaag ctgcgcagga tggagcagga gccaaatcaa gtgctattgg caacgttaga
      841 attattgata atgctgtaac agatcctaat cctgtaagac caaagaagac tatcataatt
      901 gtaataggtg ttgttttagg gctaatcgtt tcggttgtac tggtattgtt ccaagtgttt
      961 ctacgccgtg gtatcgaatc cccagagcaa ctcgaagaaa tcggaataaa cgtgtatgca
     1021 agtattccaa tatctgaatg gcttacaaaa aatgctcgtc agtcagggaa agtcagaaaa
     1081 aatcagtcag acacattgct tgctgttggt aatcctgccg atttagctgt tgaggccatt
     1141 cgtggtttac gcacaagttt gcattttgca atgatggaag caaaaaataa tgtcctaatg
     1201 atatcgggtg ctagtccaag cgcaggtatg acttttatta gcagtaactt ggcagccact
     1261 atagcaatta cagggaaaaa agttttattc attgatgctg accttcgtaa gggatatgct
     1321 cataaaatgt ttggtcataa aaacgataaa ggattatctg agtttttatc tggacaagcc
     1381 gctgctgaaa tgatcattga taaagtagaa ggggggggat ttgattacat tggtcgtgga
     1441 caaattcctc caaacccagc tgaattactt atgcatccgc gttttgaaca attattaaat
     1501 tgggcctcac aaaattatga tttaattata atagatacac ctccaatttt ggcagtgaca
     1561 gatgctgcga ttattggtcg atatgctgga acatgtctat tagtcgctcg ctttgaaaaa
     1621 aatactgtta aagaaattga tgtaagtatg aagcggtttg agcaaagtgg tgtggtagtc
     1681 aaaggctgta tacttaacgg tgttgtaaag aaagctagca gctactatag atatggccat
     1741 aatcattatg gctattctta ttatgataaa aaacaccacc accaccacca ctga
//
