LOCUS       sequence-337028-        4533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmids contain inserts (ORFs) for expressing various members 
            of the GlycoLipid Transfer Protein (GLTP) superfamily..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Simanshu DK, Zhai X, Munch D, Hofius D, Markham JE, Bielawski J, 
            Bielawska A, Malinina L, Molotkovsky JG, Mundy JW, Patel DJ, Brown 
            RE
  TITLE     Arabidopsis accelerated cell death 11, ACD11, is a 
            ceramide-1-phosphate transfer protein and intermediary regulator of 
            phytoceramide levels.
  JOURNAL   Cell Rep. 2014 Jan 30;6(2):388-99. doi: 
            10.1016/j.celrep.2013.12.023. Epub 2014 Jan 9.
  PUBMED    24412362
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.12.023"; journalName: "Cell Rep"; date: 
            "2014-01-30- 30"; volume: "6"; issue: "2"; pages: "388-99"
COMMENT     Sequence Label: pSUMO ACD11
FEATURES             Location/Qualifiers
     source          1..4533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(76..1158)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1120..1139
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1159..1236)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1282..1301
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1282..1300
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1301..1325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1381..1398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1408..1425
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1438..1731
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             2368..2412
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2450..2468)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2464..2511
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2741..3556)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2741..3556)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3464..3483
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3557..3648)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3664..4413)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(3674..3691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3914..3933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
       61 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      121 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
      181 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
      241 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
      301 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc
      361 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
      421 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
      481 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
      541 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
      601 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
      661 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
      721 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
      781 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
      841 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
      901 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
      961 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     1021 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     1081 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     1141 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     1201 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     1261 gcgactcctg cattaggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     1321 attcccctgt agaaataatt ttgtttaact ttaataagga gatataccat gggcagcagc
     1381 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccacat ggcaagcatg
     1441 tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
     1501 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
     1561 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
     1621 tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc tgaagatttg
     1681 gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg atccatggcg
     1741 gattcggaag cagataagcc actgagaaaa atctcagccg ctttcaaaaa actagcaatc
     1801 atcgtgaatt caccgaatcc ggaagttcct gtaacgcaat tctctcacgc ttgctctctg
     1861 gtctcgcctc tctttggttg ccttggaata gcttttaagt ttgcggaaat ggactatgtt
     1921 gccaaggttg atgatcttgt gagggcgtcg agttcgatat cgacattagt ggtaatgatg
     1981 gacaaagata ttgaggcaga ttgtgtaagg aaagctggta gtcatacgag aaaccttttg
     2041 agggttaagc gtggtcttga catggtcaaa gttctctttg aacagatcat agcttccgaa
     2101 ggagataact ccttgaaaga tccagcaact aagtcttatg ctcaagtgtt tgctccccac
     2161 catggatggg ctatacggaa agctgtttct cttgggatgt atgctcttcc cacaagggct
     2221 cacctactta atatgctcaa agaggatgag gcggcggcta agatacatat gcaaagctat
     2281 gtcaattcat cggcaccatt aatcacgtat cttgataatc tattcctctc caagcaactc
     2341 ggtattgatt ggtgagtcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
     2401 cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa
     2461 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
     2521 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     2581 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgacaag ctgacgaccg
     2641 ggtctccgca agtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2701 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
     2761 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     2821 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     2881 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     2941 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     3001 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     3061 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     3121 cgcggtcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     3181 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     3241 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     3301 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     3361 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     3421 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     3481 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
     3541 gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3601 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggcatgcagc
     3661 gctcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     3721 tgtcagctca ctcaaaagcg gtaatacggt tatccacaga atcaggggat aaagccggaa
     3781 agaacatgtg agcaaaaagc aaagcaccgg aagaagccaa cgccgcaggc gtttttccat
     3841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagccagag gtggcgaaac
     3901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4021 ctttctcata gctcacgctg ttggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccat tggtaactga
     4201 tttagaggac tttgtcttga agttatgcac ctgttaaggc taaactgaaa gaacagattt
     4261 tggtgagtgc ggtcctccaa cccacttacc ttggttcaaa gagttggtag ctcagcgaac
     4321 cttgagaaaa ccaccgttgg tagcggtggt ttttctttat ttatgagatg atgaatcaat
     4381 cggtctatca agtcaacgaa cagctattcc gttactctag atttcagtgc aatttatctc
     4441 ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc
     4501 atgccccgcg cccaccggaa ggagctgact ggg
//
