LOCUS       sequence-337042-       14913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP13A2 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte 
            R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen 
            JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, 
            Eggermont J, Vangheluwe P
  TITLE     ATP13A2 deficiency disrupts lysosomal polyamine export.
  JOURNAL   Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. 
            Epub 2020 Jan 29.
  PUBMED    31996848
REFERENCE   2  (bases 1 to 14913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-1968-7"; journalName: "Nature"; date: "2020-02";
            volume: "578"; issue: "7795"; pages: "419-424"
COMMENT     Sequence Label: pLenti HsATP13A2 WT
FEATURES             Location/Qualifiers
     source          1..14913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1179..1198)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1412..1431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1531..1553
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1591..1609)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1677..1781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1782..1850,1851..2642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2000..2019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2813..3401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3302..3321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3555..3572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3642..3662)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3647..3976
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3827..3962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5110..5175
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5305..5325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5750..5884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5787..5806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5841..5860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6052..6685
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6732..6857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7350..7583
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7768..7812
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8105..8222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8281..8584
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8585..8788
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8739..8759
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8785..8809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    12452..13004
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12596..12613)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12823..12842
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             13004..13606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     13503..13523
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13626..14214
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13679..13699)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14097..14108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14384..14564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actgagaggg aaagatgttg aagtaatttt cccacaatta cagcatccgt tagttacgac
       61 tctatgatct tctgacacaa attccattta ctcctcaccc tatgactcag tcgaatatat
      121 caaagttatg gacattatgc taagtaacaa attacccttt tatatagtaa atactgagta
      181 gattgagaga agaaattgtt tgcaaacctg aatagcttca agaagaagag aagtgaggat
      241 aagaataaca gttgtcattt aacaagtttt aacaagtaac ttggttagaa agggattcaa
      301 atgcataaag caagggataa atttttctgg caacaagact atacaatata accttaaata
      361 tgacttcaaa taattgttgg aacttgataa aactaattaa atattattga agattatcaa
      421 tattataaat gtaatttact tttaaaaagg gaacatagaa atgtgtatca ttagagtaga
      481 aaacaatcct tattatcaca atttgtcaaa acaagtttgt tattaacaca agtagaatac
      541 tgcattcaat taagttgact gcagattttg tgttttgtta aaattagaaa gagataacaa
      601 caatttgaat tattgaaagt aacatgtaaa tagttctaca tacgttcttt tgacatcttg
      661 ttcaatcatt gatcgaagtt ctttatcttg gaagaatttg ttccaaagac tctgaaataa
      721 ggaaaacaat ctattatata gtctcacacc tttgttttac ttttagtgat ttcaatttaa
      781 taatgtaaat ggttaaaatt tattcttctc tgagatcatt tcacattgca gatagaaaac
      841 ctgagactgg ggtaattttt attaaaatct aatttaatct cagaaacaca tctttattct
      901 aacatcaatt tttccagttt gatattatca tataaagtca gccttcctca tctgcaggtt
      961 ccacaacaaa aatccaacca actgtggatc aaaaatattg ggaaaaaatt aaaaatagca
     1021 atacaacaat aaaaaaatac aaatcagaaa aacagcacag tataacaact ttatttagca
     1081 tttacaatct attaggtatt ataagtaatc tagccagatc ctctacgccg gacgcatcgt
     1141 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     1201 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     1261 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     1321 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     1381 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1441 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     1501 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     1561 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1621 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     3661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3721 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3781 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3961 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     4021 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     4081 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     4141 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     4201 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     4261 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     4321 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     4381 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     4441 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     4501 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     4561 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     4621 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     4681 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     4741 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     4801 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     4861 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     4921 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     4981 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     5041 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     5101 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     5161 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     5221 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     5281 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     5341 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     5401 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     5461 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     5521 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     5581 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     5641 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5701 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5761 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5821 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5881 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     5941 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     6001 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     6061 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6121 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     6181 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     6241 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6301 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     6361 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     6421 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     6481 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     6541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     6721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     6781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     6841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     6901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     6961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     7021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     7081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     7141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     7201 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     7261 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     7321 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     7381 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     7441 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     7501 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     7561 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     7621 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     7681 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     7741 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     7801 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     7861 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     7921 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     7981 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     8041 attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc
     8101 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8161 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8221 ttcgggttta ttacagggac agcagagatc cactttgggg cgataagctt gggagttccg
     8281 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     8341 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     8401 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     8461 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     8521 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     8581 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     8641 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     8701 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     8761 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     8821 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaagatct gccaccatga
     8881 gcgcagacag cagccctctc gtgggcagca cgcccaccgg ttatgggacc ctgacgatag
     8941 ggacatcaat agatcccctc agctcctcag tttcatccgt gaggctcagc ggctactgtg
     9001 gcagtccatg gagggtcatc ggctatcacg tcgtggtctg gatgatggct gggatccctt
     9061 tgctgctctt ccgttggaag cccctgtggg gggtgcggct gcggctccgg ccctgcaacc
     9121 tggcccacgc cgaaacactc gttatcgaaa taagagacaa agaggatagt tcctggcagc
     9181 tcttcactgt ccaggtgcag actgaggcca tcggcgaggg cagcctggag ccgtccccac
     9241 agtcccaggc agaggatggc cggagccagg cggcagttgg ggcggtacca gagggtgcct
     9301 ggaaggatac ggcccagctc cacaagagcg aggaggcgaa gcgggtgctg cggtattacc
     9361 tcttccaggg ccagcgctat atctggatcg agacccagca agccttctac caggtcagcc
     9421 tcctggacca tggccgctct tgtgacgacg tccaccgctc ccgccatggc ctcagcctcc
     9481 aggaccaaat ggtgaggaag gccatttacg gccccaacgt gatcagcata ccggtcaagt
     9541 cctaccccca gctgctggtg gacgaggcac tgaaccccta ctatgggttc caggccttca
     9601 gcatcgcgct gtggctggct gaccactact actggtacgc cctgtgcatc ttcctcattt
     9661 cctccatctc catctgcctg tcgctgtaca agaccagaaa gcaaagccag actctaaggg
     9721 acatggtcaa gttgtccatg cgggtgtgcg tgtgccggcc agggggagag gaagagtggg
     9781 tggactccag tgagctagtg cccggagact gcctggtgct gccccaggag ggtgggctga
     9841 tgccctgtga tgccgccctg gtggccggcg agtgcatggt gaatgagagc tctctgacag
     9901 gagagagcat tccagtgctg aagacggcac tgccggaggg gctggggccc tactgtgcag
     9961 agacacaccg gcggcacaca ctcttctgcg ggaccctcat cttgcaggcc cgggcctatg
    10021 tgggaccgca cgtcctggca gtggtgaccc gcacagggtt ctgcacggca aaagggggcc
    10081 tggtgagctc catcttgcac ccccggccca tcaacttcaa gttctataaa cacagcatga
    10141 agtttgtggc tgccctctct gtcctggctc tcctcggcac catctacagc atcttcatcc
    10201 tctaccgaaa ccgggtgcct ctgaatgaga ttgtaatccg ggctctcgac ctggtgaccg
    10261 tggtggtgcc acctgccctg cctgctgcca tgactgtgtg cacgctctac gcccagagcc
    10321 gactgcggag acagggcatt ttctgcatcc acccactgcg catcaacctg gggggcaagc
    10381 tgcagctggt gtgtttcgac aagacgggca ccctcactga ggacggctta gacgtgatgg
    10441 gggtggtgcc cctgaagggg caggcattcc tgcccctggt cccagagcct cgccgcctgc
    10501 ctgtggggcc cctgctccga gcactggcca cctgccatgc cctcagccgg ctccaggaca
    10561 cccccgtggg cgaccccatg gacttgaaga tggtggagtc tactggctgg gtcctggagg
    10621 aagagccggc tgcagactca gcatttggga cccaggtctt ggcagtgatg agacccccac
    10681 tttgggagcc ccagctgcag gcaatggagg agcccccggt gccagtcagc gtcctccacc
    10741 gcttcccctt ctcttcggct ctgcagcgca tgagtgtggt ggtggcgtgg ccaggggcca
    10801 ctcagcccga ggcctacgtc aaaggctccc cggagctggt ggcagggctc tgcaaccccg
    10861 agacagtgcc caccgacttc gcccagatgc tgcagagcta tacagctgct ggctaccgtg
    10921 tcgtggccct ggccagcaag ccactgccca ctgtgcccag cctggaggca gcccagcaac
    10981 tgacgaggga cactgtggaa ggagacctga gcctcctggg gctgctggtc atgaggaacc
    11041 tactgaagcc gcagacaacg ccagttatcc aggctctgcg aaggacccgc atccgcgccg
    11101 tcatggtgac aggggacaac ctgcagacag cggtgactgt ggcccggggc tgtggcatgg
    11161 tggcccccca ggagcatctg atcatcgtcc acgccaccca ccctgagcgg ggtcagcctg
    11221 cctctctcga gttcctgccg atggagtccc ccacagccgt gaatggcgtt aaggatcctg
    11281 accaggctgc aagctacacc gtggagccag acccccgatc caggcacctg gccctcagcg
    11341 ggcccacctt tggtatcatt gtgaagcact tccccaagct gctgcccaag gtcctggtcc
    11401 agggcactgt ctttgcccgc atggcccctg agcagaagac agagctggtg tgcgagctac
    11461 agaagcttca gtactgcgtg ggcatgtgcg gagacggcgc caatgactgt ggggccctga
    11521 aggcggctga tgtcggcatc tcgctgtccc aggcagaagc ctcagtggtc tcacccttca
    11581 cctcgagcat ggccagtatt gagtgcgtgc ccatggtcat cagggagggg cgctgttccc
    11641 ttgacacttc attcagcgtc ttcaagtaca tggctctgta cagcctgacc cagttcatct
    11701 ccgtcctgat cctctacacg atcaacacca acctgggtga cctgcagttc ctggccatcg
    11761 acctggtcat caccaccaca gtggcagtgc tcatgagccg cacggggcca gcgctggtcc
    11821 tgggacgggt gcggccaccg ggggcgctgc tcagcgtgcc cgtgctcagc agcctgctgc
    11881 tgcagatggt cctggtgacc ggcgtgcagc tagggggcta cttcctgacc ctggcccagc
    11941 catggttcgt gcctctgaac aggacagtgg ccgcaccaga caacctgccc aactacgaga
    12001 acaccgtggt cttctctctg tccagcttcc agtacctcat cctggctgca gccgtgtcca
    12061 agggggcgcc cttccgccgg ccgctctaca ccaatgtgcc cttcctggtg gccctggcgc
    12121 tcctgagctc cgtcctggtg ggtcttgtcc tggtccccgg cctcctgcag gggccgctgg
    12181 cgctgaggaa catcactgac accggcttca agctgctgct gctgggtctg gtcaccctca
    12241 acttcgtggg ggccttcatg ctggagagcg tgctagacca gtgcctcccc gcctgcctgc
    12301 gccgcctccg gcccaagcgg gcctccaaga agcgcttcaa gcagctggaa cgagagctgg
    12361 ccgagcagcc ctggccgccg ctgcccgccg gccccctgag gtagactagt acgcgtgctc
    12421 tagcccaatt ccgccccctc ccccccccct aacgttactg gccgaagccg cttggaataa
    12481 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
    12541 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
    12601 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
    12661 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
    12721 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
    12781 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
    12841 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
    12901 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
    12961 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatgggca ccgagtacaa
    13021 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
    13081 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
    13141 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    13201 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
    13261 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    13321 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    13381 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    13441 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    13501 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    13561 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggcg cgttgaattc
    13621 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
    13681 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
    13741 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
    13801 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
    13861 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
    13921 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
    13981 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
    14041 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
    14101 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
    14161 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg
    14221 aattctgtcg actggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    14281 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaaaatc
    14341 gtcgagagat gctgcatata agcagctgct ttttgcttgt actgggtctc tctggttaga
    14401 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    14461 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    14521 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
    14581 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
    14641 acattataat agatttagca ggaattgaac taggagtgga gcacacaggc aaagctgcag
    14701 aagtacttgg aagaagccac cagagatact cacgattctg cacatacctg gctaatccca
    14761 gatcctaagg attacattaa gtttactaac atttatataa tgatttatag tttaaagtat
    14821 aaacttatct aatttactat tctgacagat attaattaat cctcaaatat cataagagat
    14881 gattactatt atccccattt aacacaagag gaa
//
