LOCUS       sequence-337043-       15802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Martin S, Smolders S, Van den Haute C, Heeman B, van Veen S, 
            Crosiers D, Beletchi I, Verstraeten A, Gossye H, Gelders G, Pals P, 
            Hamouda NN, Engelborghs S, Martin JJ, Eggermont J, De Deyn PP, Cras 
            P, Baekelandt V, Vangheluwe P, Van Broeckhoven C
  TITLE     Mutated ATP10B increases Parkinson's disease risk by compromising 
            lysosomal glucosylceramide export.
  JOURNAL   Acta Neuropathol. 2020 Jun;139(6):1001-1024. doi: 
            10.1007/s00401-020-02145-7. Epub 2020 Mar 14.
  PUBMED    32172343
REFERENCE   2  (bases 1 to 15802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-020-02145-7"; journalName: "Acta Neuropathol"; date:
            "2020-06"; volume: "139"; issue: "6"; pages: "1001-1024"
COMMENT     Sequence Label: pLenti HsATP10B WT
FEATURES             Location/Qualifiers
     source          1..15802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1350..1369)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1583..1602)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1702..1724
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1762..1780)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1848..1952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1953..2021,2022..2813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2171..2190)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2984..3572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3473..3492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3726..3743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3813..3833)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3818..4147
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3998..4133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5281..5346
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5476..5496
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5921..6055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5958..5977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6012..6031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6223..6856
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6903..7028
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7521..7754
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7939..7983
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8276..8393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8452..8755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8756..8959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8910..8930
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8956..8980
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9048..9057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10089..10100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    13512..14064
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13656..13673)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13883..13902
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             14064..14666
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     14563..14583
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14686..15274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14739..14759)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15157..15168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             15444..15624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 catacctggc taatcccaga tcctaaggat tacattaagt ttactaacat ttatataatg
       61 atttatagtt taaagtataa acttatctaa tttactattc tgacagatat taattaatcc
      121 tcaaatatca taagagatga ttactattat ccccatttaa cacaagagga aactgagagg
      181 gaaagatgtt gaagtaattt tcccacaatt acagcatccg ttagttacga ctctatgatc
      241 ttctgacaca aattccattt actcctcacc ctatgactca gtcgaatata tcaaagttat
      301 ggacattatg ctaagtaaca aattaccctt ttatatagta aatactgagt agattgagag
      361 aagaaattgt ttgcaaacct gaatagcttc aagaagaaga gaagtgagga taagaataac
      421 agttgtcatt taacaagttt taacaagtaa cttggttaga aagggattca aatgcataaa
      481 gcaagggata aatttttctg gcaacaagac tatacaatat aaccttaaat atgacttcaa
      541 ataattgttg gaacttgata aaactaatta aatattattg aagattatca atattataaa
      601 tgtaatttac ttttaaaaag ggaacataga aatgtgtatc attagagtag aaaacaatcc
      661 ttattatcac aatttgtcaa aacaagtttg ttattaacac aagtagaata ctgcattcaa
      721 ttaagttgac tgcagatttt gtgttttgtt aaaattagaa agagataaca acaatttgaa
      781 ttattgaaag taacatgtaa atagttctac atacgttctt ttgacatctt gttcaatcat
      841 tgatcgaagt tctttatctt ggaagaattt gttccaaaga ctctgaaata aggaaaacaa
      901 tctattatat agtctcacac ctttgtttta cttttagtga tttcaattta ataatgtaaa
      961 tggttaaaat ttattcttct ctgagatcat ttcacattgc agatagaaaa cctgagactg
     1021 gggtaatttt tattaaaatc taatttaatc tcagaaacac atctttattc taacatcaat
     1081 ttttccagtt tgatattatc atataaagtc agccttcctc atctgcaggt tccacaacaa
     1141 aaatccaacc aactgtggat caaaaatatt gggaaaaaat taaaaatagc aatacaacaa
     1201 taaaaaaata caaatcagaa aaacagcaca gtataacaac tttatttagc atttacaatc
     1261 tattaggtat tataagtaat ctagccagat cctctacgcc ggacgcatcg tggccggcat
     1321 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     1381 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     1441 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     1501 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     1561 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     1621 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     1681 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     1741 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1801 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2161 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2461 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3781 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     3841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3901 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3961 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4021 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4081 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4141 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     4201 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     4261 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     4321 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     4381 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     4441 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     4501 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     4561 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     4621 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     4681 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     4741 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     4801 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     4861 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     4921 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     4981 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     5041 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     5101 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     5161 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     5221 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     5281 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     5341 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     5401 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     5461 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     5521 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     5581 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     5641 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     5701 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     5761 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     5821 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5881 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     6061 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     6121 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     6181 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     6241 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     6301 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     6361 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     6421 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     6481 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     6541 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     6601 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     6661 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6721 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6781 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6841 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     6901 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     6961 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7021 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7081 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7141 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7201 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7261 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7321 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     7381 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     7441 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7501 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7561 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     7621 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     7681 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     7741 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     7801 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     7861 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     7921 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     7981 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8041 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8101 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     8161 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     8221 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
     8281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     8341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     8401 attacaggga cagcagagat ccactttggg gcgataagct tgggagttcc gcgttacata
     8461 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     8521 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     8581 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     8641 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     8701 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     8761 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     8821 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     8881 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     8941 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     9001 ctgttttgac ctccatagaa gacaccgact ctagaggatc tgctagcgcc accatggccc
     9061 tctcagtgga ctcatcgtgg catcggtggc agtggagagt cagagatggc ttcccccatt
     9121 gtccatcgga aaccacaccg ctgctctctc cagagaaagg gagacagagc tacaacttga
     9181 cacagcagcg ggtcgtgttc cccaacaaca gcatattcca tcaagattgg gaagaggtct
     9241 ccaggagata ccctggcaac agaacctgca caaccaaata caccctcttc accttcctgc
     9301 cccggaatct ctttgagcaa tttcatagat gggctaacct ctatttcctg ttcctggtga
     9361 ttttgaactg gatgccctcc atggaagtct tccacagaga aatcaccatg ttaccattgg
     9421 ccattgtcct gttcgtcatc atgatcaagg atggcatgga ggacttcaag agacaccgct
     9481 ttgataaagc aataaactgc tccaacattc gaatttatga aagaaaagag cagacctatg
     9541 tgcagaagtg ctggaaggat gtgcgcgtgg gagacttcat ccaaatgaaa tgcaatgaga
     9601 ttgtcccagc agacatactc ctcctttttt cctctgaccc caatgggata tgccatctgg
     9661 aaactgccag cttggatgga gagacaaacc tcaagcaaag atgtgtcgtg aagggcttct
     9721 cacagcagga ggtacagttc gaaccagagc ttttccacaa taccatcgtg tgtgagaaac
     9781 ccaacaacca cctcaacaaa tttaagggtt atatggagca tcctgaccag accaggactg
     9841 gctttggctg tgagagtctt ctgcttcgag gctgcaccat cagaaacacc gagatggctg
     9901 ttggcattgt catctatgca ggccatgaga cgaaagccat gctgaacaac agtggccccc
     9961 ggtacaaacg cagcaagatt gagcggcgca tgaatataga catcttcttc tgcattggga
    10021 tcctcatcct catgtgcctt attggagctg taggtcacag catctggaat gggacctttg
    10081 aagaacaccc tcccttcgat gtgccagatg ccaatggcag cttccttccc agtgcccttg
    10141 ggggcttcta catgttcctc acaatgatca tcctgctcca ggtgctgatc cccatctctt
    10201 tgtatgtctc cattgagctg gtgaagctcg ggcaagtgtt cttcttgagc aatgaccttg
    10261 acctgtatga tgaagagacc gatttatcca ttcaatgtcg agccctcaac atcgcagagg
    10321 acttgggcca gatccagtac atcttctccg ataagacggg gaccctgaca gagaacaaga
    10381 tggtgttccg acgttgcacc atcatgggca gcgagtattc tcaccaagaa aatgctaagc
    10441 gactggagac cccaaaggag ctggactcag atggtgaaga gtggacccaa taccaatgcc
    10501 tgtccttctc ggctagatgg gcccaggatc cagcaactat gagaagccaa aaaggtgctc
    10561 agcctctgag gaggagccag agtgcccggg tgcccatcca gggccactac cggcaaaggt
    10621 ctatggggca ccgtgaaagc tcacagcctc ctgtggcctt cagcagctcc atagaaaaag
    10681 atgtaactcc agataaaaac ctactgacca aggttcgaga tgctgccctg tggttggaga
    10741 ccttgtcaga cagcagacct gccaaggctt ccctctccac cacctcctcc attgctgatt
    10801 tcttccttgc cttaaccatc tgcaactctg tcatggtgtc cacaaccacc gagcccaggc
    10861 agagggtcac catcaaaccc tcaagcaagg ctctggggac gtccctggag aagattcagc
    10921 agctcttcca gaagttgaag ctattgagcc tcagccagtc attctcatcc actgcaccct
    10981 ctgacacaga cctcggggag agcttagggg ccaacgtggc caccacagac tcggatgaga
    11041 gagatgatgc atctgtgtgc agtggaggtg actccactga tgacggtggc tacaggagca
    11101 gcatgtggga ccagggcgac atcctggagt ctgggtcagg cacttccttg gaggaggcat
    11161 tggaggcccc agccacagac ctggccaggc ctgagttctg ttacgaggct gagagccctg
    11221 atgaggccgc cctggtgcac gctgcccatg cctacagctt cacactagtg tcccggacac
    11281 ctgagcaggt gactgtgcgc ctgccccagg gcacctgcct caccttcagc ctcctctgca
    11341 ccctgggctt tgactctgtc aggaagagaa tgtctgtggt tgtgaggcac ccactgactg
    11401 gcgagattgt tgtctacacc aagggtgctg actcggtcat catggacctg ctggaagacc
    11461 cagcctgcgt acctgacatt aatatggaaa agaagctgag aaaaatccga gcccggaccc
    11521 aaaagcatct agacttgtat gcaagagatg gcctgcgcac actatgcatt gccaagaagg
    11581 ttgtaagcga agaggacttc cggagatggg ccagtttccg gcgtgaggct gaggcatccc
    11641 tcgacaaccg agatgagctt ctcatggaaa ctgcacagca tctggagaat caactcacct
    11701 tacttggagc cactgggatc gaagaccggc tgcaggaagg agttccagat acgattgcca
    11761 ctctgcggga ggctgggatc cagctctggg tcctgactgg agataagcag gagacagcgg
    11821 tcaacattgc ccattcctgc agactgttaa atcagaccga cactgtttat accatcaata
    11881 cagagaatca ggagacctgt gaatccatcc tcaattgtgc attggaagag ctaaagcaat
    11941 ttcgtgaact acagaagcca gaccgcaagc tctttggatt ccgcttacct tccaagacac
    12001 catccatcac ctcagaagct gtggttccag aagctggatt ggtcatcgat gggaagacat
    12061 tgaatgccat cttccaggga aagctagaga agaagtttct ggaattgacc cagtattgtc
    12121 ggtccgtcct gtgctgccgc tccacgccac tccagaagag tatgatagtc aagctggtgc
    12181 gagacaagtt gcgcgtcatg accctttcca taggtgatgg agcaaatgat gtaagcatga
    12241 ttcaagctgc tgatattgga attggaatat ctggacagga aggcatgcag gctgtcatgt
    12301 ccagcgactt tgccatcacc cgctttaagc atctcaagaa gttgctgctc gtgcatggcc
    12361 actggtgtta ctcgcgcctg gccaggatgg tggtgtacta cctctacaag aacgtgtgct
    12421 acgtcaacct gctcttctgg tatcagttct tctgtggttt ctccagctcc accatgattg
    12481 attactggca gatgatattc ttcaatctct tctttacctc cttgcctcct cttgtctttg
    12541 gagtccttga caaagacatc tctgcagaaa cactcctggc attgcctgag ctatacaaga
    12601 gtggccagaa ctctgagtgc tataacctgt cgactttctg gatttctatg gtggatgcat
    12661 tctaccagag cctcatctgt ttctttatcc cttacctggc ctataagggc tctgatatag
    12721 atgtctttac ctttgggaca ccaatcaaca ccatctccct caccacaatc cttttgcacc
    12781 aggcaatgga aatgaagaca tggaccattt tccacggagt cgtgctcctc ggcagcttcc
    12841 tgatgtactt tctggtatcc ctcctgtaca atgccacctg cgtcatctgc aacagcccca
    12901 ccaatcccta ttgggtgatg gaaggccagc tctcaaaccc cactttctac ctcgtctgct
    12961 ttctcacacc agttgttgct cttctcccaa gatacttttt cctgtctctg caaggaactt
    13021 gtgggaagtc tctaatctca aaagctcaga aaattgacaa actcccccca gacaaaagaa
    13081 acctggaaat ccagagttgg agaagcagac agaggcctgc ccctgtcccc gaagtggctc
    13141 gaccaactca ccacccagtg tcatctatca caggacagga cttcagtgcc agcaccccaa
    13201 agagctctaa ccctcccaag aggaagcatg tggaagagtc agtactccac gaacagagat
    13261 gtggcacgga gtgcatgagg gatgactcat gctcagggga ctcctcagct caactctcat
    13321 ccggggagca cctgctggga cctaacagga taatggccta ctcaagagga cagactgata
    13381 tgtgccggtg ctcaaagagg agcagccatc gccgatccca gagttcactg accatatagt
    13441 acctcgagca ctagtccccg ggaccgcggc acgcgtgctc tagcccaatt ccgccccctc
    13501 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
    13561 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
    13621 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
    13681 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
    13741 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
    13801 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
    13861 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
    13921 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
    13981 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
    14041 cctttgaaaa acacgatgat aatatgggca ccgagtacaa gcccacggtg cgcctcgcca
    14101 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
    14161 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
    14221 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
    14281 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
    14341 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
    14401 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
    14461 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
    14521 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
    14581 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
    14641 gcatgacccg caagcccggt gcctgaggcg cgttgaattc ccgataatca acctctggat
    14701 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    14761 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    14821 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    14881 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    14941 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    15001 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    15061 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
    15121 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    15181 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    15241 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattctgtcg actggtacct
    15301 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    15361 ggactggaag ggctaattca ctcccaacga agacaaaatc gtcgagagat gctgcatata
    15421 agcagctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    15481 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    15541 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    15601 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
    15661 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattataat agatttagca
    15721 ggaattgaac taggagtgga gcacacaggc aaagctgcag aagtacttgg aagaagccac
    15781 cagagatact cacgattctg ca
//
