LOCUS       sequence-337045-       13007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing 
            TMEM30A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Martin S, Smolders S, Van den Haute C, Heeman B, van Veen S, 
            Crosiers D, Beletchi I, Verstraeten A, Gossye H, Gelders G, Pals P, 
            Hamouda NN, Engelborghs S, Martin JJ, Eggermont J, De Deyn PP, Cras 
            P, Baekelandt V, Vangheluwe P, Van Broeckhoven C
  TITLE     Mutated ATP10B increases Parkinson's disease risk by compromising 
            lysosomal glucosylceramide export.
  JOURNAL   Acta Neuropathol. 2020 Jun;139(6):1001-1024. doi: 
            10.1007/s00401-020-02145-7. Epub 2020 Mar 14.
  PUBMED    32172343
REFERENCE   2  (bases 1 to 13007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-020-02145-7"; journalName: "Acta Neuropathol"; date:
            "2020-06"; volume: "139"; issue: "6"; pages: "1001-1024"
COMMENT     Sequence Label: pLenti TMEM30A
FEATURES             Location/Qualifiers
     source          1..13007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1141..1160)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1374..1393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1493..1515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1553..1571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1639..1743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1744..1812,1813..2604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1962..1981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2775..3363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3264..3283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3517..3534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3604..3624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3609..3938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3789..3924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5072..5137
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5267..5287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5712..5846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5749..5768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5803..5822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6014..6647
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6694..6819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7312..7545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7730..7774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8067..8184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8243..8546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8547..8750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8701..8721
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8747..8771
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8855..8872
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8873..8896
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8906..8915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(10196..10213)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10423..10442
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10610..11635
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10610..11635
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    11682..12270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11735..11755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12153..12164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12440..12620
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tacagcatcc gttagttacg actctatgat cttctgacac aaattccatt tactcctcac
       61 cctatgactc agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct
      121 tttatatagt aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt
      181 caagaagaag agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta
      241 acttggttag aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga
      301 ctatacaata taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt
      361 aaatattatt gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag
      421 aaatgtgtat cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt
      481 gttattaaca caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt
      541 taaaattaga aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta
      601 catacgttct tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt
      661 tgttccaaag actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt
      721 acttttagtg atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca
      781 tttcacattg cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat
      841 ctcagaaaca catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt
      901 cagccttcct catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat
      961 tgggaaaaaa ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac
     1021 agtataacaa ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga
     1081 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     1141 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     1201 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     1261 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     1321 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     1381 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     1441 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     1501 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     1561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3601 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3661 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3901 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     3961 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     4021 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     4081 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     4141 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     4201 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     4261 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     4321 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     4381 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     4441 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     4501 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     4561 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     4621 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     4681 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     4741 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     4801 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     4861 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     4921 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     4981 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     5041 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     5101 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     5161 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     5221 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     5281 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     5341 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     5401 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     5461 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     5521 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     5581 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     5641 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5701 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5821 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     5881 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     5941 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     6001 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     6061 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     6121 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     6181 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     6241 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     6301 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     6361 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     6421 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     6481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6661 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6721 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6781 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6841 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     6901 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     6961 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7021 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7081 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7141 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7201 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7261 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7321 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7381 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7441 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7501 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7561 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7621 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7681 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7741 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7801 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7861 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     7921 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     7981 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
     8041 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     8101 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     8161 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     8221 ggcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8281 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     8341 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     8401 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     8461 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     8521 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     8581 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     8641 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     8701 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     8761 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac
     8821 tctagctaga ggatctagag gatccgccac catgcaccat caccatcacc atgactacaa
     8881 ggacgacgat gacaagcttg gatctgccac catggcgatg aactataacg cgaaggatga
     8941 agtggacggt gggcccccgt gtgctccggg gggcaccgcg aagactcgga gaccggataa
     9001 cacggccttc aaacagcaac ggctgccagc ttggcagccc atccttacgg ctggcacggt
     9061 gctacctatt ttcttcatca tcggtctcat cttcattccc atcggcattg gcatctttgt
     9121 cacctccaac aacatccgcg agatcgagat tgattatacc ggaacagagc cttccagtcc
     9181 ctgtaataaa tgtttatctc cggatgtgac accttgcttt tgtaccatta acttcacact
     9241 ggaaaagtca tttgagggca acgtgtttat gtattatgga ctgtctaatt tctatcaaaa
     9301 ccatcgtcgt tacgtgaaat ctcgtgatga tagtcaacta aatggagatt ctagtgcttt
     9361 gcttaatccc agtaaggaat gtgaacctta tcgaagaaat gaagacaaac caattgctcc
     9421 ttgtggagct attgccaaca gcatgtttaa tgatacatta gaattgtttc tcattggcaa
     9481 tgattcttat cctataccta tcgctttgaa aaagaaaggt attgcttggt ggacagataa
     9541 aaatgtgaaa ttcagaaatc cccctggagg agacaacctg gaagaacgat ttaaaggtac
     9601 aacaaagcct gtgaactggc ttaaaccagt ttacatgctg gattctgacc cagataataa
     9661 tggattcata aatgaggatt ttattgtttg gatgcgtact gcagcattac ctacttttcg
     9721 caagttgtat cgtcttatag aaaggaaaag tgatttacat ccaacattac cagctggccg
     9781 atactctttg aatgtcacat acaattaccc tgtacattat tttgatggac gaaaacggat
     9841 gatcttgagc actatttcat ggatgggagg aaaaaatcca tttttgggga ttgcttacat
     9901 cgctgttggt tccatctcct tccttctggg agttgtactg ctagtaatta atcataaata
     9961 tagaaacagt agtaatacag ctgacattac catttaactc gagactagta cgcgtgtcga
    10021 gaagcttttt gaattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa
    10081 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
    10141 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
    10201 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
    10261 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
    10321 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
    10381 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
    10441 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
    10501 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
    10561 aaccacgggg acgtggtttt cctttgaaaa acacgatgat cagcacgtga tgaaaaagcc
    10621 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
    10681 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
    10741 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
    10801 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
    10861 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
    10921 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
    10981 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
    11041 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
    11101 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
    11161 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
    11221 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
    11281 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
    11341 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
    11401 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
    11461 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
    11521 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
    11581 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aataggtcga
    11641 gcactagtcc ccgggaccgc ggcacgcgtt gaattcccga taatcaacct ctggattaca
    11701 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
    11761 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
    11821 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
    11881 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
    11941 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
    12001 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    12061 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
    12121 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    12181 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    12241 gtcggatctc cctttgggcc gcctccccgc atcgggaatt ctgtcgactg gtacctttaa
    12301 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    12361 tggaagggct aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca
    12421 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    12481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    12541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    12601 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
    12661 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
    12721 ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga
    12781 gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt
    12841 actaacattt atataatgat ttatagttta aagtataaac ttatctaatt tactattctg
    12901 acagatatta attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca
    12961 caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaat
//
