LOCUS       sequence-337048-       14913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP13A2 D508N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte 
            R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen 
            JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, 
            Eggermont J, Vangheluwe P
  TITLE     ATP13A2 deficiency disrupts lysosomal polyamine export.
  JOURNAL   Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. 
            Epub 2020 Jan 29.
  PUBMED    31996848
REFERENCE   2  (bases 1 to 14913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-1968-7"; journalName: "Nature"; date: "2020-02";
            volume: "578"; issue: "7795"; pages: "419-424"
COMMENT     Sequence Label: pLenti HsATP13A2 D508N
FEATURES             Location/Qualifiers
     source          1..14913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..154
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             316..918
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     815..835
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    938..1526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(991..1011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1409..1420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1696..1876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3404..3423)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3637..3656)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3756..3778
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3816..3834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3902..4006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4007..4075,4076..4867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4225..4244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5038..5626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5527..5546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5780..5797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5867..5887)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5872..6201
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6052..6187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7335..7400
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7530..7550
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7975..8109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8012..8031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8066..8085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8277..8910
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8957..9082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9575..9808
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9993..10037
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10330..10447
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10506..10809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10810..11013
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10964..10984
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11010..11034
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(14677..14913,1..316)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(14821..14838)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
       61 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      121 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      181 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
      241 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      301 aaacacgatg ataatatggg caccgagtac aagcccacgg tgcgcctcgc cacccgcgac
      361 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
      421 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
      481 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
      541 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
      601 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
      661 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
      721 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
      781 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
      841 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
      901 cgcaagcccg gtgcctgagg cgcgttgaat tcccgataat caacctctgg attacaaaat
      961 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     1021 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     1081 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     1141 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     1201 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     1261 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     1321 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     1381 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     1441 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     1501 gatctccctt tgggccgcct ccccgcatcg ggaattctgt cgactggtac ctttaagacc
     1561 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     1621 agggctaatt cactcccaac gaagacaaaa tcgtcgagag atgctgcata taagcagctg
     1681 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     1741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     1801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     1861 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     1921 aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag caggaattga
     1981 actaggagtg gagcacacag gcaaagctgc agaagtactt ggaagaagcc accagagata
     2041 ctcacgattc tgcacatacc tggctaatcc cagatcctaa ggattacatt aagtttacta
     2101 acatttatat aatgatttat agtttaaagt ataaacttat ctaatttact attctgacag
     2161 atattaatta atcctcaaat atcataagag atgattacta ttatccccat ttaacacaag
     2221 aggaaactga gagggaaaga tgttgaagta attttcccac aattacagca tccgttagtt
     2281 acgactctat gatcttctga cacaaattcc atttactcct caccctatga ctcagtcgaa
     2341 tatatcaaag ttatggacat tatgctaagt aacaaattac ccttttatat agtaaatact
     2401 gagtagattg agagaagaaa ttgtttgcaa acctgaatag cttcaagaag aagagaagtg
     2461 aggataagaa taacagttgt catttaacaa gttttaacaa gtaacttggt tagaaaggga
     2521 ttcaaatgca taaagcaagg gataaatttt tctggcaaca agactataca atataacctt
     2581 aaatatgact tcaaataatt gttggaactt gataaaacta attaaatatt attgaagatt
     2641 atcaatatta taaatgtaat ttacttttaa aaagggaaca tagaaatgtg tatcattaga
     2701 gtagaaaaca atccttatta tcacaatttg tcaaaacaag tttgttatta acacaagtag
     2761 aatactgcat tcaattaagt tgactgcaga ttttgtgttt tgttaaaatt agaaagagat
     2821 aacaacaatt tgaattattg aaagtaacat gtaaatagtt ctacatacgt tcttttgaca
     2881 tcttgttcaa tcattgatcg aagttcttta tcttggaaga atttgttcca aagactctga
     2941 aataaggaaa acaatctatt atatagtctc acacctttgt tttactttta gtgatttcaa
     3001 tttaataatg taaatggtta aaatttattc ttctctgaga tcatttcaca ttgcagatag
     3061 aaaacctgag actggggtaa tttttattaa aatctaattt aatctcagaa acacatcttt
     3121 attctaacat caatttttcc agtttgatat tatcatataa agtcagcctt cctcatctgc
     3181 aggttccaca acaaaaatcc aaccaactgt ggatcaaaaa tattgggaaa aaattaaaaa
     3241 tagcaataca acaataaaaa aatacaaatc agaaaaacag cacagtataa caactttatt
     3301 tagcatttac aatctattag gtattataag taatctagcc agatcctcta cgccggacgc
     3361 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     3421 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     3481 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     3541 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     3601 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     3661 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3721 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3781 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3841 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3901 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3961 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4021 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4081 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4141 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4201 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4261 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4321 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4381 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4441 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4501 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4561 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4621 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4681 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4741 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4801 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4861 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4921 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5821 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     5881 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5941 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6001 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6061 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6121 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6181 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     6241 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     6301 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     6361 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     6421 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     6481 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     6541 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     6601 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     6661 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     6721 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     6781 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     6841 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     6901 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     6961 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     7021 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     7081 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     7141 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     7201 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     7261 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     7321 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     7381 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     7441 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     7501 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     7561 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     7621 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     7681 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     7741 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     7801 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     7861 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     7921 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8101 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     8161 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     8221 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     8281 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     8341 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     8401 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     8461 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     8521 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     8581 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     8641 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     8701 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8761 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8821 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8881 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8941 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     9001 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     9061 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     9121 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     9181 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     9241 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9301 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     9361 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     9421 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     9481 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9541 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9601 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9661 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9721 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9781 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9841 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9901 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9961 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10021 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10081 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10141 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10201 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10261 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt
    10321 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    10381 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10441 aaattttcgg gtttattaca gggacagcag agatccactt tggggcgata agcttgggag
    10501 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    10561 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    10621 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    10681 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    10741 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    10801 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    10861 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    10921 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    10981 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
    11041 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagaa gatctgccac
    11101 catgagcgca gacagcagcc ctctcgtggg cagcacgccc accggttatg ggaccctgac
    11161 gatagggaca tcaatagatc ccctcagctc ctcagtttca tccgtgaggc tcagcggcta
    11221 ctgtggcagt ccatggaggg tcatcggcta tcacgtcgtg gtctggatga tggctgggat
    11281 ccctttgctg ctcttccgtt ggaagcccct gtggggggtg cggctgcggc tccggccctg
    11341 caacctggcc cacgccgaaa cactcgttat cgaaataaga gacaaagagg atagttcctg
    11401 gcagctcttc actgtccagg tgcagactga ggccatcggc gagggcagcc tggagccgtc
    11461 cccacagtcc caggcagagg atggccggag ccaggcggca gttggggcgg taccagaggg
    11521 tgcctggaag gatacggccc agctccacaa gagcgaggag gcgaagcggg tgctgcggta
    11581 ttacctcttc cagggccagc gctatatctg gatcgagacc cagcaagcct tctaccaggt
    11641 cagcctcctg gaccatggcc gctcttgtga cgacgtccac cgctcccgcc atggcctcag
    11701 cctccaggac caaatggtga ggaaggccat ttacggcccc aacgtgatca gcataccggt
    11761 caagtcctac ccccagctgc tggtggacga ggcactgaac ccctactatg ggttccaggc
    11821 cttcagcatc gcgctgtggc tggctgacca ctactactgg tacgccctgt gcatcttcct
    11881 catttcctcc atctccatct gcctgtcgct gtacaagacc agaaagcaaa gccagactct
    11941 aagggacatg gtcaagttgt ccatgcgggt gtgcgtgtgc cggccagggg gagaggaaga
    12001 gtgggtggac tccagtgagc tagtgcccgg agactgcctg gtgctgcccc aggagggtgg
    12061 gctgatgccc tgtgatgccg ccctggtggc cggcgagtgc atggtgaatg agagctctct
    12121 gacaggagag agcattccag tgctgaagac ggcactgccg gaggggctgg ggccctactg
    12181 tgcagagaca caccggcggc acacactctt ctgcgggacc ctcatcttgc aggcccgggc
    12241 ctatgtggga ccgcacgtcc tggcagtggt gacccgcaca gggttctgca cggcaaaagg
    12301 gggcctggtg agctccatct tgcacccccg gcccatcaac ttcaagttct ataaacacag
    12361 catgaagttt gtggctgccc tctctgtcct ggctctcctc ggcaccatct acagcatctt
    12421 catcctctac cgaaaccggg tgcctctgaa tgagattgta atccgggctc tcgacctggt
    12481 gaccgtggtg gtgccacctg ccctgcctgc tgccatgact gtgtgcacgc tctacgccca
    12541 gagccgactg cggagacagg gcattttctg catccaccca ctgcgcatca acctgggggg
    12601 caagctgcag ctggtgtgtt tcaacaagac gggcaccctc actgaggacg gcttagacgt
    12661 gatgggggtg gtgcccctga aggggcaggc attcctgccc ctggtcccag agcctcgccg
    12721 cctgcctgtg gggcccctgc tccgagcact ggccacctgc catgccctca gccggctcca
    12781 ggacaccccc gtgggcgacc ccatggactt gaagatggtg gagtctactg gctgggtcct
    12841 ggaggaagag ccggctgcag actcagcatt tgggacccag gtcttggcag tgatgagacc
    12901 cccactttgg gagccccagc tgcaggcaat ggaggagccc ccggtgccag tcagcgtcct
    12961 ccaccgcttc cccttctctt cggctctgca gcgcatgagt gtggtggtgg cgtggccagg
    13021 ggccactcag cccgaggcct acgtcaaagg ctccccggag ctggtggcag ggctctgcaa
    13081 ccccgagaca gtgcccaccg acttcgccca gatgctgcag agctatacag ctgctggcta
    13141 ccgtgtcgtg gccctggcca gcaagccact gcccactgtg cccagcctgg aggcagccca
    13201 gcaactgacg agggacactg tggaaggaga cctgagcctc ctggggctgc tggtcatgag
    13261 gaacctactg aagccgcaga caacgccagt tatccaggct ctgcgaagga cccgcatccg
    13321 cgccgtcatg gtgacagggg acaacctgca gacagcggtg actgtggccc ggggctgtgg
    13381 catggtggcc ccccaggagc atctgatcat cgtccacgcc acccaccctg agcggggtca
    13441 gcctgcctct ctcgagttcc tgccgatgga gtcccccaca gccgtgaatg gcgttaagga
    13501 tcctgaccag gctgcaagct acaccgtgga gccagacccc cgatccaggc acctggccct
    13561 cagcgggccc acctttggta tcattgtgaa gcacttcccc aagctgctgc ccaaggtcct
    13621 ggtccagggc actgtctttg cccgcatggc ccctgagcag aagacagagc tggtgtgcga
    13681 gctacagaag cttcagtact gcgtgggcat gtgcggagac ggcgccaatg actgtggggc
    13741 cctgaaggcg gctgatgtcg gcatctcgct gtcccaggca gaagcctcag tggtctcacc
    13801 cttcacctcg agcatggcca gtattgagtg cgtgcccatg gtcatcaggg aggggcgctg
    13861 ttcccttgac acttcattca gcgtcttcaa gtacatggct ctgtacagcc tgacccagtt
    13921 catctccgtc ctgatcctct acacgatcaa caccaacctg ggtgacctgc agttcctggc
    13981 catcgacctg gtcatcacca ccacagtggc agtgctcatg agccgcacgg ggccagcgct
    14041 ggtcctggga cgggtgcggc caccgggggc gctgctcagc gtgcccgtgc tcagcagcct
    14101 gctgctgcag atggtcctgg tgaccggcgt gcagctaggg ggctacttcc tgaccctggc
    14161 ccagccatgg ttcgtgcctc tgaacaggac agtggccgca ccagacaacc tgcccaacta
    14221 cgagaacacc gtggtcttct ctctgtccag cttccagtac ctcatcctgg ctgcagccgt
    14281 gtccaagggg gcgcccttcc gccggccgct ctacaccaat gtgcccttcc tggtggccct
    14341 ggcgctcctg agctccgtcc tggtgggtct tgtcctggtc cccggcctcc tgcaggggcc
    14401 gctggcgctg aggaacatca ctgacaccgg cttcaagctg ctgctgctgg gtctggtcac
    14461 cctcaacttc gtgggggcct tcatgctgga gagcgtgcta gaccagtgcc tccccgcctg
    14521 cctgcgccgc ctccggccca agcgggcctc caagaagcgc ttcaagcagc tggaacgaga
    14581 gctggccgag cagccctggc cgccgctgcc cgccggcccc ctgaggtaga ctagtacgcg
    14641 tgctctagcc caattccgcc ccctcccccc cccctaacgt tactggccga agccgcttgg
    14701 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
    14761 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
    14821 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
    14881 cttcttgaag acaaacaacg tctgtagcga ccc
//
