LOCUS       sequence-337050-       14913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP13A2 D962N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   van Veen S, Martin S, Van den Haute C, Benoy V, Lyons J, Vanhoutte 
            R, Kahler JP, Decuypere JP, Gelders G, Lambie E, Zielich J, Swinnen 
            JV, Annaert W, Agostinis P, Ghesquiere B, Verhelst S, Baekelandt V, 
            Eggermont J, Vangheluwe P
  TITLE     ATP13A2 deficiency disrupts lysosomal polyamine export.
  JOURNAL   Nature. 2020 Feb;578(7795):419-424. doi: 10.1038/s41586-020-1968-7. 
            Epub 2020 Jan 29.
  PUBMED    31996848
REFERENCE   2  (bases 1 to 14913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-1968-7"; journalName: "Nature"; date: "2020-02";
            volume: "578"; issue: "7795"; pages: "419-424"
COMMENT     Sequence Label: pLenti HsATP13A2 D962N
FEATURES             Location/Qualifiers
     source          1..14913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1208..1227)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1441..1460)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1560..1582
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1620..1638)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1706..1810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1811..1879,1880..2671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2029..2048)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2842..3430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3331..3350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3584..3601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3671..3691)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3676..4005
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3856..3991
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5139..5204
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5334..5354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5779..5913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5816..5835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5870..5889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6081..6714
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6761..6886
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7379..7612
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7797..7841
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8134..8251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8310..8613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8614..8817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8768..8788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8814..8838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    12481..13033
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12625..12642)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12852..12871
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             13033..13635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     13532..13552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13655..14243
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13708..13728)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14126..14137)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14413..14593
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actattatcc ccatttaaca caagaggaaa ctgagaggga aagatgttga agtaattttc
       61 ccacaattac agcatccgtt agttacgact ctatgatctt ctgacacaaa ttccatttac
      121 tcctcaccct atgactcagt cgaatatatc aaagttatgg acattatgct aagtaacaaa
      181 ttaccctttt atatagtaaa tactgagtag attgagagaa gaaattgttt gcaaacctga
      241 atagcttcaa gaagaagaga agtgaggata agaataacag ttgtcattta acaagtttta
      301 acaagtaact tggttagaaa gggattcaaa tgcataaagc aagggataaa tttttctggc
      361 aacaagacta tacaatataa ccttaaatat gacttcaaat aattgttgga acttgataaa
      421 actaattaaa tattattgaa gattatcaat attataaatg taatttactt ttaaaaaggg
      481 aacatagaaa tgtgtatcat tagagtagaa aacaatcctt attatcacaa tttgtcaaaa
      541 caagtttgtt attaacacaa gtagaatact gcattcaatt aagttgactg cagattttgt
      601 gttttgttaa aattagaaag agataacaac aatttgaatt attgaaagta acatgtaaat
      661 agttctacat acgttctttt gacatcttgt tcaatcattg atcgaagttc tttatcttgg
      721 aagaatttgt tccaaagact ctgaaataag gaaaacaatc tattatatag tctcacacct
      781 ttgttttact tttagtgatt tcaatttaat aatgtaaatg gttaaaattt attcttctct
      841 gagatcattt cacattgcag atagaaaacc tgagactggg gtaattttta ttaaaatcta
      901 atttaatctc agaaacacat ctttattcta acatcaattt ttccagtttg atattatcat
      961 ataaagtcag ccttcctcat ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca
     1021 aaaatattgg gaaaaaatta aaaatagcaa tacaacaata aaaaaataca aatcagaaaa
     1081 acagcacagt ataacaactt tatttagcat ttacaatcta ttaggtatta taagtaatct
     1141 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     1201 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     1261 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     1321 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     1381 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     1441 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1501 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     1561 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     1621 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1741 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1801 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1861 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1921 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1981 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2041 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2101 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2161 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2221 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2281 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2341 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2401 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2461 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2521 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2581 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2641 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3541 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3601 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3661 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3721 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3841 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3961 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     4021 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     4081 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     4141 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     4201 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     4261 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     4321 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     4381 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     4441 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     4501 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     4561 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     4621 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     4681 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     4741 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     4801 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     4861 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     4921 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     4981 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     5041 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     5101 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     5161 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     5221 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     5281 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     5341 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     5401 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     5461 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     5521 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     5581 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     5641 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     5701 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     5761 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5881 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     5941 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     6001 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     6061 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     6121 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     6181 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     6241 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     6301 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     6361 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     6421 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     6481 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     6541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     6721 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     6781 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     6841 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     6901 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     6961 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7021 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7081 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7141 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     7201 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     7261 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     7321 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     7381 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     7441 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     7501 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     7561 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     7621 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     7681 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     7741 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     7801 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     7861 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     7921 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     7981 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     8041 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     8101 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     8161 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8221 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8281 actttggggc gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8341 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8401 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8461 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     8521 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     8581 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     8641 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     8701 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     8761 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     8821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     8881 caccgactct agaagatctg ccaccatgag cgcagacagc agccctctcg tgggcagcac
     8941 gcccaccggt tatgggaccc tgacgatagg gacatcaata gatcccctca gctcctcagt
     9001 ttcatccgtg aggctcagcg gctactgtgg cagtccatgg agggtcatcg gctatcacgt
     9061 cgtggtctgg atgatggctg ggatcccttt gctgctcttc cgttggaagc ccctgtgggg
     9121 ggtgcggctg cggctccggc cctgcaacct ggcccacgcc gaaacactcg ttatcgaaat
     9181 aagagacaaa gaggatagtt cctggcagct cttcactgtc caggtgcaga ctgaggccat
     9241 cggcgagggc agcctggagc cgtccccaca gtcccaggca gaggatggcc ggagccaggc
     9301 ggcagttggg gcggtaccag agggtgcctg gaaggatacg gcccagctcc acaagagcga
     9361 ggaggcgaag cgggtgctgc ggtattacct cttccagggc cagcgctata tctggatcga
     9421 gacccagcaa gccttctacc aggtcagcct cctggaccat ggccgctctt gtgacgacgt
     9481 ccaccgctcc cgccatggcc tcagcctcca ggaccaaatg gtgaggaagg ccatttacgg
     9541 ccccaacgtg atcagcatac cggtcaagtc ctacccccag ctgctggtgg acgaggcact
     9601 gaacccctac tatgggttcc aggccttcag catcgcgctg tggctggctg accactacta
     9661 ctggtacgcc ctgtgcatct tcctcatttc ctccatctcc atctgcctgt cgctgtacaa
     9721 gaccagaaag caaagccaga ctctaaggga catggtcaag ttgtccatgc gggtgtgcgt
     9781 gtgccggcca gggggagagg aagagtgggt ggactccagt gagctagtgc ccggagactg
     9841 cctggtgctg ccccaggagg gtgggctgat gccctgtgat gccgccctgg tggccggcga
     9901 gtgcatggtg aatgagagct ctctgacagg agagagcatt ccagtgctga agacggcact
     9961 gccggagggg ctggggccct actgtgcaga gacacaccgg cggcacacac tcttctgcgg
    10021 gaccctcatc ttgcaggccc gggcctatgt gggaccgcac gtcctggcag tggtgacccg
    10081 cacagggttc tgcacggcaa aagggggcct ggtgagctcc atcttgcacc cccggcccat
    10141 caacttcaag ttctataaac acagcatgaa gtttgtggct gccctctctg tcctggctct
    10201 cctcggcacc atctacagca tcttcatcct ctaccgaaac cgggtgcctc tgaatgagat
    10261 tgtaatccgg gctctcgacc tggtgaccgt ggtggtgcca cctgccctgc ctgctgccat
    10321 gactgtgtgc acgctctacg cccagagccg actgcggaga cagggcattt tctgcatcca
    10381 cccactgcgc atcaacctgg ggggcaagct gcagctggtg tgtttcgaca agacgggcac
    10441 cctcactgag gacggcttag acgtgatggg ggtggtgccc ctgaaggggc aggcattcct
    10501 gcccctggtc ccagagcctc gccgcctgcc tgtggggccc ctgctccgag cactggccac
    10561 ctgccatgcc ctcagccggc tccaggacac ccccgtgggc gaccccatgg acttgaagat
    10621 ggtggagtct actggctggg tcctggagga agagccggct gcagactcag catttgggac
    10681 ccaggtcttg gcagtgatga gacccccact ttgggagccc cagctgcagg caatggagga
    10741 gcccccggtg ccagtcagcg tcctccaccg cttccccttc tcttcggctc tgcagcgcat
    10801 gagtgtggtg gtggcgtggc caggggccac tcagcccgag gcctacgtca aaggctcccc
    10861 ggagctggtg gcagggctct gcaaccccga gacagtgccc accgacttcg cccagatgct
    10921 gcagagctat acagctgctg gctaccgtgt cgtggccctg gccagcaagc cactgcccac
    10981 tgtgcccagc ctggaggcag cccagcaact gacgagggac actgtggaag gagacctgag
    11041 cctcctgggg ctgctggtca tgaggaacct actgaagccg cagacaacgc cagttatcca
    11101 ggctctgcga aggacccgca tccgcgccgt catggtgaca ggggacaacc tgcagacagc
    11161 ggtgactgtg gcccggggct gtggcatggt ggccccccag gagcatctga tcatcgtcca
    11221 cgccacccac cctgagcggg gtcagcctgc ctctctcgag ttcctgccga tggagtcccc
    11281 cacagccgtg aatggcgtta aggatcctga ccaggctgca agctacaccg tggagccaga
    11341 cccccgatcc aggcacctgg ccctcagcgg gcccaccttt ggtatcattg tgaagcactt
    11401 ccccaagctg ctgcccaagg tcctggtcca gggcactgtc tttgcccgca tggcccctga
    11461 gcagaagaca gagctggtgt gcgagctaca gaagcttcag tactgcgtgg gcatgtgcgg
    11521 agacggcgcc aatgactgtg gggccctgaa ggcggctgat gtcggcatct cgctgtccca
    11581 ggcagaagcc tcagtggtct cacccttcac ctcgagcatg gccagtattg agtgcgtgcc
    11641 catggtcatc agggaggggc gctgttccct tgacacttca ttcagcgtct tcaagtacat
    11701 ggctctgtac agcctgaccc agttcatctc cgtcctgatc ctctacacga tcaacaccaa
    11761 cctgggtgac ctgcagttcc tggccatcaa tctggtcatc accaccacag tggcagtgct
    11821 catgagccgc acggggccag cgctggtcct gggacgggtg cggccaccgg gggcgctgct
    11881 cagcgtgccc gtgctcagca gcctgctgct gcagatggtc ctggtgaccg gcgtgcagct
    11941 agggggctac ttcctgaccc tggcccagcc atggttcgtg cctctgaaca ggacagtggc
    12001 cgcaccagac aacctgccca actacgagaa caccgtggtc ttctctctgt ccagcttcca
    12061 gtacctcatc ctggctgcag ccgtgtccaa gggggcgccc ttccgccggc cgctctacac
    12121 caatgtgccc ttcctggtgg ccctggcgct cctgagctcc gtcctggtgg gtcttgtcct
    12181 ggtccccggc ctcctgcagg ggccgctggc gctgaggaac atcactgaca ccggcttcaa
    12241 gctgctgctg ctgggtctgg tcaccctcaa cttcgtgggg gccttcatgc tggagagcgt
    12301 gctagaccag tgcctccccg cctgcctgcg ccgcctccgg cccaagcggg cctccaagaa
    12361 gcgcttcaag cagctggaac gagagctggc cgagcagccc tggccgccgc tgcccgccgg
    12421 ccccctgagg tagactagta cgcgtgctct agcccaattc cgccccctcc ccccccccta
    12481 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
    12541 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
    12601 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
    12661 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
    12721 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
    12781 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
    12841 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
    12901 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
    12961 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
    13021 cacgatgata atatgggcac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
    13081 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
    13141 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
    13201 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
    13261 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
    13321 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
    13381 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
    13441 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
    13501 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
    13561 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
    13621 aagcccggtg cctgaggcgc gttgaattcc cgataatcaa cctctggatt acaaaatttg
    13681 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    13741 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    13801 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    13861 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    13921 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    13981 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    14041 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
    14101 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    14161 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    14221 ctccctttgg gccgcctccc cgcatcggga attctgtcga ctggtacctt taagaccaat
    14281 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    14341 gctaattcac tcccaacgaa gacaaaatcg tcgagagatg ctgcatataa gcagctgctt
    14401 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    14461 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    14521 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    14581 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
    14641 aaatgaatat cagagagtga gaggccttga cattataata gatttagcag gaattgaact
    14701 aggagtggag cacacaggca aagctgcaga agtacttgga agaagccacc agagatactc
    14761 acgattctgc acatacctgg ctaatcccag atcctaagga ttacattaag tttactaaca
    14821 tttatataat gatttatagt ttaaagtata aacttatcta atttactatt ctgacagata
    14881 ttaattaatc ctcaaatatc ataagagatg att
//
