LOCUS       sequence-337053-       15802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B D433N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Martin S, Smolders S, Van den Haute C, Heeman B, van Veen S, 
            Crosiers D, Beletchi I, Verstraeten A, Gossye H, Gelders G, Pals P, 
            Hamouda NN, Engelborghs S, Martin JJ, Eggermont J, De Deyn PP, Cras 
            P, Baekelandt V, Vangheluwe P, Van Broeckhoven C
  TITLE     Mutated ATP10B increases Parkinson's disease risk by compromising 
            lysosomal glucosylceramide export.
  JOURNAL   Acta Neuropathol. 2020 Jun;139(6):1001-1024. doi: 
            10.1007/s00401-020-02145-7. Epub 2020 Mar 14.
  PUBMED    32172343
REFERENCE   2  (bases 1 to 15802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-020-02145-7"; journalName: "Acta Neuropathol"; date:
            "2020-06"; volume: "139"; issue: "6"; pages: "1001-1024"
COMMENT     Sequence Label: pLenti HsATP10B D433N
FEATURES             Location/Qualifiers
     source          1..15802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     196..215
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             377..979
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     876..896
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    999..1587
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1052..1072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1470..1481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1757..1937
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3465..3484)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3698..3717)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3817..3839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3877..3895)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3963..4067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4068..4136,4137..4928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4286..4305)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5099..5687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5588..5607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5841..5858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5928..5948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5933..6262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6113..6248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7396..7461
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7591..7611
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8036..8170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8073..8092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8127..8146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8338..8971
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9018..9143
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9636..9869
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10054..10098
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10391..10508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10567..10870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10871..11074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11025..11045
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11071..11095
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      11163..11172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(12204..12215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(15627..15802,1..377)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(15771..15788)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
       61 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
      121 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
      181 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
      241 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
      301 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      361 aaaacacgat gataatatgg gcaccgagta caagcccacg gtgcgcctcg ccacccgcga
      421 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
      481 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
      541 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
      601 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
      661 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
      721 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
      781 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
      841 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
      901 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
      961 ccgcaagccc ggtgcctgag gcgcgttgaa ttcccgataa tcaacctctg gattacaaaa
     1021 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     1081 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     1141 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     1201 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     1261 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     1321 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1381 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     1441 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     1501 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     1561 ggatctccct ttgggccgcc tccccgcatc gggaattctg tcgactggta cctttaagac
     1621 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     1681 aagggctaat tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat ataagcagct
     1741 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1801 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1861 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1921 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     1981 aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg
     2041 aactaggagt ggagcacaca ggcaaagctg cagaagtact tggaagaagc caccagagat
     2101 actcacgatt ctgcacatac ctggctaatc ccagatccta aggattacat taagtttact
     2161 aacatttata taatgattta tagtttaaag tataaactta tctaatttac tattctgaca
     2221 gatattaatt aatcctcaaa tatcataaga gatgattact attatcccca tttaacacaa
     2281 gaggaaactg agagggaaag atgttgaagt aattttccca caattacagc atccgttagt
     2341 tacgactcta tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga
     2401 atatatcaaa gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac
     2461 tgagtagatt gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt
     2521 gaggataaga ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg
     2581 attcaaatgc ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct
     2641 taaatatgac ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat
     2701 tatcaatatt ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag
     2761 agtagaaaac aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta
     2821 gaatactgca ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga
     2881 taacaacaat ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac
     2941 atcttgttca atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg
     3001 aaataaggaa aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca
     3061 atttaataat gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata
     3121 gaaaacctga gactggggta atttttatta aaatctaatt taatctcaga aacacatctt
     3181 tattctaaca tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg
     3241 caggttccac aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa
     3301 atagcaatac aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat
     3361 ttagcattta caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg
     3421 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     3481 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     3541 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     3601 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     3661 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     3721 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3781 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3841 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3901 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3961 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4021 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4081 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4141 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4201 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4261 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4321 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4381 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4441 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4501 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4561 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4621 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4681 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4741 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4801 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4861 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4921 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4981 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5041 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5221 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5881 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     5941 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6001 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6061 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6121 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6181 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6241 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     6301 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     6361 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     6421 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     6481 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     6541 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     6601 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     6661 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     6721 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     6781 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     6841 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     6901 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     6961 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     7021 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     7081 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     7141 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     7201 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     7261 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     7321 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     7381 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     7441 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     7501 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     7561 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     7621 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     7681 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     7741 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     7801 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     7861 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     7921 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     7981 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     8041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8161 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     8221 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     8281 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     8341 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     8401 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     8461 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     8521 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8581 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     8641 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     8701 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8761 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8941 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     9001 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     9061 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9121 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9181 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9241 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9301 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9361 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9421 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9481 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9541 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9601 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     9661 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     9721 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9781 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9841 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9901 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9961 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    10021 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    10081 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    10141 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    10201 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    10261 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    10321 agatccattc gattagtgaa cggatctcga cggtatcgcc gaattcacaa atggcagtat
    10381 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10441 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    10501 aaaattttcg ggtttattac agggacagca gagatccact ttggggcgat aagcttggga
    10561 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    10621 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    10681 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    10741 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10801 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10861 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
    10921 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
    10981 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
    11041 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
    11101 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctaga ggatctgcta
    11161 gcgccaccat ggccctctca gtggactcat cgtggcatcg gtggcagtgg agagtcagag
    11221 atggcttccc ccattgtcca tcggaaacca caccgctgct ctctccagag aaagggagac
    11281 agagctacaa cttgacacag cagcgggtcg tgttccccaa caacagcata ttccatcaag
    11341 attgggaaga ggtctccagg agataccctg gcaacagaac ctgcacaacc aaatacaccc
    11401 tcttcacctt cctgccccgg aatctctttg agcaatttca tagatgggct aacctctatt
    11461 tcctgttcct ggtgattttg aactggatgc cctccatgga agtcttccac agagaaatca
    11521 ccatgttacc attggccatt gtcctgttcg tcatcatgat caaggatggc atggaggact
    11581 tcaagagaca ccgctttgat aaagcaataa actgctccaa cattcgaatt tatgaaagaa
    11641 aagagcagac ctatgtgcag aagtgctgga aggatgtgcg cgtgggagac ttcatccaaa
    11701 tgaaatgcaa tgagattgtc ccagcagaca tactcctcct tttttcctct gaccccaatg
    11761 ggatatgcca tctggaaact gccagcttgg atggagagac aaacctcaag caaagatgtg
    11821 tcgtgaaggg cttctcacag caggaggtac agttcgaacc agagcttttc cacaatacca
    11881 tcgtgtgtga gaaacccaac aaccacctca acaaatttaa gggttatatg gagcatcctg
    11941 accagaccag gactggcttt ggctgtgaga gtcttctgct tcgaggctgc accatcagaa
    12001 acaccgagat ggctgttggc attgtcatct atgcaggcca tgagacgaaa gccatgctga
    12061 acaacagtgg cccccggtac aaacgcagca agattgagcg gcgcatgaat atagacatct
    12121 tcttctgcat tgggatcctc atcctcatgt gccttattgg agctgtaggt cacagcatct
    12181 ggaatgggac ctttgaagaa caccctccct tcgatgtgcc agatgccaat ggcagcttcc
    12241 ttcccagtgc ccttgggggc ttctacatgt tcctcacaat gatcatcctg ctccaggtgc
    12301 tgatccccat ctctttgtat gtctccattg agctggtgaa gctcgggcaa gtgttcttct
    12361 tgagcaatga ccttgacctg tatgatgaag agaccgattt atccattcaa tgtcgagccc
    12421 tcaacatcgc agaggacttg ggccagatcc agtacatctt ctccaataag acggggaccc
    12481 tgacagagaa caagatggtg ttccgacgtt gcaccatcat gggcagcgag tattctcacc
    12541 aagaaaatgc taagcgactg gagaccccaa aggagctgga ctcagatggt gaagagtgga
    12601 cccaatacca atgcctgtcc ttctcggcta gatgggccca ggatccagca actatgagaa
    12661 gccaaaaagg tgctcagcct ctgaggagga gccagagtgc ccgggtgccc atccagggcc
    12721 actaccggca aaggtctatg gggcaccgtg aaagctcaca gcctcctgtg gccttcagca
    12781 gctccataga aaaagatgta actccagata aaaacctact gaccaaggtt cgagatgctg
    12841 ccctgtggtt ggagaccttg tcagacagca gacctgccaa ggcttccctc tccaccacct
    12901 cctccattgc tgatttcttc cttgccttaa ccatctgcaa ctctgtcatg gtgtccacaa
    12961 ccaccgagcc caggcagagg gtcaccatca aaccctcaag caaggctctg gggacgtccc
    13021 tggagaagat tcagcagctc ttccagaagt tgaagctatt gagcctcagc cagtcattct
    13081 catccactgc accctctgac acagacctcg gggagagctt aggggccaac gtggccacca
    13141 cagactcgga tgagagagat gatgcatctg tgtgcagtgg aggtgactcc actgatgacg
    13201 gtggctacag gagcagcatg tgggaccagg gcgacatcct ggagtctggg tcaggcactt
    13261 ccttggagga ggcattggag gccccagcca cagacctggc caggcctgag ttctgttacg
    13321 aggctgagag ccctgatgag gccgccctgg tgcacgctgc ccatgcctac agcttcacac
    13381 tagtgtcccg gacacctgag caggtgactg tgcgcctgcc ccagggcacc tgcctcacct
    13441 tcagcctcct ctgcaccctg ggctttgact ctgtcaggaa gagaatgtct gtggttgtga
    13501 ggcacccact gactggcgag attgttgtct acaccaaggg tgctgactcg gtcatcatgg
    13561 acctgctgga agacccagcc tgcgtacctg acattaatat ggaaaagaag ctgagaaaaa
    13621 tccgagcccg gacccaaaag catctagact tgtatgcaag agatggcctg cgcacactat
    13681 gcattgccaa gaaggttgta agcgaagagg acttccggag atgggccagt ttccggcgtg
    13741 aggctgaggc atccctcgac aaccgagatg agcttctcat ggaaactgca cagcatctgg
    13801 agaatcaact caccttactt ggagccactg ggatcgaaga ccggctgcag gaaggagttc
    13861 cagatacgat tgccactctg cgggaggctg ggatccagct ctgggtcctg actggagata
    13921 agcaggagac agcggtcaac attgcccatt cctgcagact gttaaatcag accgacactg
    13981 tttataccat caatacagag aatcaggaga cctgtgaatc catcctcaat tgtgcattgg
    14041 aagagctaaa gcaatttcgt gaactacaga agccagaccg caagctcttt ggattccgct
    14101 taccttccaa gacaccatcc atcacctcag aagctgtggt tccagaagct ggattggtca
    14161 tcgatgggaa gacattgaat gccatcttcc agggaaagct agagaagaag tttctggaat
    14221 tgacccagta ttgtcggtcc gtcctgtgct gccgctccac gccactccag aagagtatga
    14281 tagtcaagct ggtgcgagac aagttgcgcg tcatgaccct ttccataggt gatggagcaa
    14341 atgatgtaag catgattcaa gctgctgata ttggaattgg aatatctgga caggaaggca
    14401 tgcaggctgt catgtccagc gactttgcca tcacccgctt taagcatctc aagaagttgc
    14461 tgctcgtgca tggccactgg tgttactcgc gcctggccag gatggtggtg tactacctct
    14521 acaagaacgt gtgctacgtc aacctgctct tctggtatca gttcttctgt ggtttctcca
    14581 gctccaccat gattgattac tggcagatga tattcttcaa tctcttcttt acctccttgc
    14641 ctcctcttgt ctttggagtc cttgacaaag acatctctgc agaaacactc ctggcattgc
    14701 ctgagctata caagagtggc cagaactctg agtgctataa cctgtcgact ttctggattt
    14761 ctatggtgga tgcattctac cagagcctca tctgtttctt tatcccttac ctggcctata
    14821 agggctctga tatagatgtc tttacctttg ggacaccaat caacaccatc tccctcacca
    14881 caatcctttt gcaccaggca atggaaatga agacatggac cattttccac ggagtcgtgc
    14941 tcctcggcag cttcctgatg tactttctgg tatccctcct gtacaatgcc acctgcgtca
    15001 tctgcaacag ccccaccaat ccctattggg tgatggaagg ccagctctca aaccccactt
    15061 tctacctcgt ctgctttctc acaccagttg ttgctcttct cccaagatac tttttcctgt
    15121 ctctgcaagg aacttgtggg aagtctctaa tctcaaaagc tcagaaaatt gacaaactcc
    15181 ccccagacaa aagaaacctg gaaatccaga gttggagaag cagacagagg cctgcccctg
    15241 tccccgaagt ggctcgacca actcaccacc cagtgtcatc tatcacagga caggacttca
    15301 gtgccagcac cccaaagagc tctaaccctc ccaagaggaa gcatgtggaa gagtcagtac
    15361 tccacgaaca gagatgtggc acggagtgca tgagggatga ctcatgctca ggggactcct
    15421 cagctcaact ctcatccggg gagcacctgc tgggacctaa caggataatg gcctactcaa
    15481 gaggacagac tgatatgtgc cggtgctcaa agaggagcag ccatcgccga tcccagagtt
    15541 cactgaccat atagtacctc gagcactagt ccccgggacc gcggcacgcg tgctctagcc
    15601 caattccgcc ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
    15661 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
    15721 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
    15781 aggaatgcaa ggtctgttga at
//
