LOCUS       sequence-337068-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and purification of mature (no signal sequence) human 
            alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. 
            coli BL21(DE3) cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Krishnan B, Hedstrom L, Hebert DN, Gierasch LM, Gershenson A
  TITLE     Expression and Purification of Active Recombinant Human Alpha-1 
            Antitrypsin (AAT) from Escherichia coli.
  JOURNAL   Methods Mol Biol. 2017;1639:195-209. doi: 
            10.1007/978-1-4939-7163-3_19.
  PUBMED    28752459
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2017"; volume: "1639:195-209. doi"; pages: " 
            10.1007/978-1-4939-7163-3_19"
COMMENT     Sequence Label: pEAT8-137 M1V
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggatcccc agggagatgc tgcccagaag acagatacat cccaccatga tcaggatcac
       61 ccaaccttca acaagatcac ccccaacctg gctgagttcg ccttcagcct ataccgccag
      121 ctggcacacc agtccaacag caccaatatc ttcttctccc cagtgagcat cgctacagcc
      181 tttgcaatgc tctccctggg gaccaaggct gacactcacg atgaaatcct ggagggcctg
      241 aatttcaacc tcacggagat tccggaggct cagatccatg aaggcttcca ggaactcctc
      301 cgtaccctca accagccaga cagccagctc cagctgacca ccggcaatgg cctgttcctc
      361 agcgagggcc tgaagctagt ggataagttt ttggaggacg tcaaaaagct gtaccactca
      421 gaagccttca ctgtcaactt cggggacacc gaagaggcca agaaacagat caacgattac
      481 gtggagaagg gtactcaagg gaaaattgtg gatttggtca aggagcttga cagagacaca
      541 gtttttgctc tggtgaatta catcttcttt aaaggcaaat gggagagacc ctttgaagtc
      601 aaggacaccg aggaagagga cttccacgtg gaccaggtga ccaccgtgaa ggtgcctatg
      661 atgaagcgtt taggcatgtt taacatccag cactgtaaga agctgtccag ctgggtgctg
      721 ctgatgaaat acctgggcaa tgccaccgcc atcttcttcc tgcctgatga ggggaaacta
      781 cagcacctgg aaaatgaact cacccacgat atcatcacca agttcctgga aaatgaagac
      841 agaaggtctg ccagcttaca tttacccaaa ctgtccatta ctggaaccta tgatctgaag
      901 agcgtcctgg gtcaactggg catcactaag gtcttcagca atggggctga cctctccggg
      961 gtcacagagg aggcacccct gaagctctcc aaggccgtgc ataaggctgt gctgaccatc
     1021 gacgagaaag ggactgaagc tgctggggcc atgtttttag aggccatacc catgtctatc
     1081 ccccccgagg tcaagttcaa caaacccttt gtcttcttaa tgattgaaca aaataccaag
     1141 tctcccctct tcatgggaaa agtggtgaat cccacccaaa aataa
//
