LOCUS       sequence-337069-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and purification of the mature form of Bacillus 
            thuringiensis phosphaatidyl inositol specific phospholipase C 
            (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   He T, Gershenson A, Eyles SJ, Lee YJ, Liu WR, Wang J, Gao J, Roberts
            MF
  TITLE     Fluorinated Aromatic Amino Acids Distinguish Cation-pi Interactions 
            from Membrane Insertion.
  JOURNAL   J Biol Chem. 2015 Jul 31;290(31):19334-42. doi: 
            10.1074/jbc.M115.668343. Epub 2015 Jun 19.
  PUBMED    26092728
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.668343"; journalName: "J Biol Chem"; date: 
            "2015-07-31- 31"; volume: "290"; issue: "31"; pages: "19334-42"
COMMENT     Sequence Label: pBtPIPLC His
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             904..921
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctagct ctgttaatga gcttgaaaat tggtcaaaat ggatgcaacc tatacctgat
       61 aatatcccgt tagcacgaat ttcaattcca ggaacacacg atagtgggac gttcaagttg
      121 caaaatccga ttaagcaagt gtggggaatg acgcaagaat atgattttcg ctatcaaatg
      181 gaccatggag ctcgcatttt tgatataaga ggacgtttaa cagatgataa tacgatagtt
      241 cttcatcatg ggccattata tctttacgta acactgcatg aattcataaa tgaagcgaaa
      301 caatttttaa aagataaccc gagtgaaaca attattatgt ctttaaaaaa agagtatgag
      361 gatatgaaag gggcagaagg ttcatttagt agtacgtttg aaaaaaatta ttttgttgat
      421 cctatctttt taaaaacaga aggaaatata aaacttggag atgctcgtgg gaaaattgta
      481 ctactaaaaa gatatagtgg tagtaatgaa tctggaggat ataataattt ttattggcca
      541 gataatgaga cgtttaccac aactgtaaac caaaatgtaa atgtaacagt acaagataaa
      601 tataaagtga attatgatga gaaagtaaaa tctattaaag atacgatgga tgaaacgatg
      661 aacaatagcg aggatttaaa tcatctatat attaatttta caagcttgtc ttctggtggt
      721 acagcatgga atagtccata ttactacgct tcttatataa atcctgaaat tgcaaacgat
      781 ataaaacaaa agaatcctac aagagtaggc tgggtaattc aagactacat aaatgaaaag
      841 tggtcaccat tattgtatca agaagttata agagcgaata agtcattaat aaaagaactc
      901 gagcaccacc accaccacca ctga
//
