LOCUS       sequence-337071-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and purification of mature, His-tagged Staphylococcus 
            aureus (strain FPR3757) phosphaatidyl inositol specific 
            phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Goldstein R, Cheng J, Stec B, Roberts MF
  TITLE     Structure of the S. aureus PI-specific phospholipase C reveals 
            modulation of active site access by a titratable pi-cation latched 
            loop.
  JOURNAL   Biochemistry. 2012 Mar 27;51(12):2579-87. doi: 10.1021/bi300057q. 
            Epub 2012 Mar 16.
  PUBMED    22390775
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi300057q";
            journalName: "Biochemistry"; date: "2012-03-27- 27"; volume: "51"; 
            issue: "12"; pages: "2579-87"
COMMENT     Sequence Label: pSaPIPLC His
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..33
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             961..978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctagca tgactggtgg acagcaaatg ggtcgcggat ccgaattctc agattcgttg
       61 agtaaaagtc cagaaaattg gatgagtaaa cttgatgatg gaaaacattt aactgagatt
      121 aatataccgg gttcacatga tagtggctca ttcactttaa aggatccagt aaaatcagtt
      181 tgggcaaaga ctcaagataa agattacctt acccaaatga agtcgggagt caggtttttt
      241 gatattagag gtagagcaag tgctgataat atgatttcag ttcatcacgg catggtttat
      301 ttgcatcatg aattaggaaa atttctcgat gatgctaaat attacttgag tgcttatcca
      361 aacgaaacaa ttgtgatgtc tatgaaaaag gactacgata gcgattctaa agttacgaag
      421 acatttgaag aaatttttag agaatattat tataataacc cgcaatatca gaatcttttt
      481 tacacaggaa gtaatgcgaa tcctacttta aaagaaacga aaggtaaaat tgtcctattc
      541 aatagaatgg ggggtacgta cataaaaagt ggttatggtg ctgacacgtc aggtattcaa
      601 tgggcagaca atgcgacatt tgaaacgaaa attaataatg gtagcttaaa tttaaaagta
      661 caagatgagt ataaagatta ctatgataaa aaagttgaag ctgttaaaaa tttattggct
      721 aaagctaaaa cggatagtaa caaagacaat gtatatgtga atttcttgag tgtagcgtct
      781 ggaggcagcg catttaatag tacttataac tatgcatcac atataaatcc tgaaattgca
      841 aaaacgatta aagcaaatgg gaaagctaga acgggttggc tgattgttga ctatgcagga
      901 tatacgtggc ctggatatga tgatatcgta agtgaaatta tagatagtaa taaactcgag
      961 caccaccacc accaccactg a
//
