LOCUS       sequence-33709-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB535
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(170..256)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
       61 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
      121 gagtcagtga gcgaggaagc ggaatatatc cctaggtcta gggcggcgga tttgtcctac
      181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      241 cctttcgttt tatttgatgc ctctagatca gctaattaag cttggctgca ggtcgacatc
      301 ttagcgggtc aggtcgtcaa aaaacttctt cacaccatca aagaagctct ttgagcgcgg
      361 gctgttgtgc tcgccggttg ggccaccgaa gctttcttgc agctcttgca gcagctgttt
      421 ctgcctttcg ttcaggccta ccggtgtttc gacgacaacg cggcacagca aatcaccctg
      481 tgcgccaccg cggacagact tgacgccttt accgcgcata cggaatagct taccggtctg
      541 ggtttcgcca ggcactttca gtttgacgcg accatcaagg gtcggtactt cgatttcgcc
      601 acccagcgcc gccatagcga agttgatcgg gacttcgcaa tacaggttgt tgccttcacg
      661 ctcgaaaatc gggtgctgtt taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc
      721 atgctcgccc gcttcacctt cgcccgcaag acggatgcgg tctccagtgt ccacccctgc
      781 cgggatttta acggacagcg tttt
//
