LOCUS       sequence-33710-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB535
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             203..214
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg
       61 gtgtcaacgt aaatgcatgc cgcttcgcct tcgcgcgcga attgtcgaga aaatttatca
      121 aaaagagtgt tgacttgtga gcggataaca atgatactta gattcaattg tgagcggata
      181 acaatttcac acagaattca ttaaagagga gaaaggatcc atgggtaaaa taattggtat
      241 cgacctgggt actaccaact cttgtgtagc gattatggat ggcaccactc ctcgcgtgct
      301 ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt gcctataccc aggatggtga
      361 aactctagtt ggtcagccgg ctaaacgtca ggcagtgacg aacccgcaaa acactctgtt
      421 tgcgattaaa cgcctgattg gtcgccgctt ccaggacgaa gaagtacagc gtgatgtttc
      481 catcatgccg ttcaaaatta ttgctgctga taacggcgac gcatgggtcg aagttaaagg
      541 ccagaaaatg gcaccgccgc agatttctgc tgaagtgctg aaaaaaatga agaaaaccgc
      601 tgaagattac ctgggtgaac cggtaactga agctgttatc accgtaccgg catactttaa
      661 cgatgctcag cgtcaggcaa ccaaagacgc agg
//
