LOCUS       sequence-337117-        3540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag human PLD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-hPLD3
FEATURES             Location/Qualifiers
     source          1..3540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        39..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     619..643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          732..828
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             844..861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             889..918
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             919..948
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             955..966
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2307..2441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2331..2350)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2385..2404
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2484..3072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2564..2583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3102..3476)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3477..3540)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtcgcg aggccatatg ggttaaccca tggccgttga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgtcgagc
      661 cgagtggttg tgcctccata gaagccacca tgacccgcct gaccgtgctg gccctgctgg
      721 ccggcctgct ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      781 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc cagcagccgc
      841 gcccatcatc atcatcatca tggtccgcta gttgacgtcg ccagcaatga acaaaaactc
      901 atctcagaag aggatctggc tagtatgact ggtggacagc aaatgggtcg ggatatcgaa
      961 ggtcgtgggc tagctgaata cggcgacttg gggctagctg aatacggcga cttgcatctc
     1021 tttgggccca accagcgccc agccccctgc tatgaccctt gcgaagcagt gctggtggaa
     1081 agcattcctg agggcctgga cttccccaat gcctccacgg ggaacccttc caccagccag
     1141 gcctggctgg gcctgctcgc cggtgcgcac agcagcctgg acatcgcctc cttctactgg
     1201 accctcacca acaatgacac ccacacgcag gagccctctg cccagcaggg tgaggaggtc
     1261 ctccggcagc tgcagaccct ggcaccaaag ggcgtgaacg tccgcatcgc tgtgagcaag
     1321 cccagcgggc cccagccaca ggcggacctg caggctctgc tgcagagcgg tgcccaggtc
     1381 cgcatggtgg acatgcagaa gctgacccat ggcgtcctgc ataccaagtt ctgggtggtg
     1441 gaccagaccc acttctacct gggcagtgcc aacatggact ggcgttcact gacccaggtc
     1501 aaggagctgg gcgtggtcat gtacaactgc agctgcctgg ctcgagacct gaccaagatc
     1561 tttgaggcct actggttcct gggccaggca ggcagctcca tcccatcaac ttggccccgg
     1621 ttctatgaca cccgctacaa ccaagagaca ccaatggaga tctgcctcaa tggaacccct
     1681 gctctggcct acctggcgag tgcgccccca cccctgtgtc caagtggccg cactccagac
     1741 ctgaaggctc tactcaacgt ggtggacaat gcccggagtt tcatctacgt cgctgtcatg
     1801 aactacctgc ccactctgga gttctcccac cctcacaggt tctggcctgc cattgacgat
     1861 gggctgcggc gggccaccta cgagcgtggc gtcaaggtgc gcctgctcat cagctgctgg
     1921 ggacactcgg agccatccat gcgggccttc ctgctctctc tggctgccct gcgtgacaac
     1981 catacccact ctgacatcca ggtgaaactc tttgtggtcc ccgcggatga ggcccaggct
     2041 cgaatcccat atgcccgtgt caaccacaac aagtacatgg tgactgaacg cgccacctac
     2101 atcggaacct ccaactggtc tggcaactac ttcacggaga cggcgggcac ctcgctgctg
     2161 gtgacgcaga atgggagggg cggcctgcgg agccagctgg aggccatttt cctgagggac
     2221 tgggactccc cttacagcca tgaccttgac acctcagctg acagcgtggg caacgcctgc
     2281 cgcctgctct gataaatcta gaagctgatc cagacatgat aagatacatt gatgagtttg
     2341 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2401 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggatatcct cgagactagt
     2461 gggcccgttt aaacacatgt gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3061 aaaggatctc aagaagagaa gatcctttga tcttttctac gtcagtcctg ctcctcggcc
     3121 acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc
     3181 tcgccgatct cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc
     3241 gaccactcgg cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc
     3301 ggcaccacct ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg
     3361 gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc
     3421 gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatactc
     3481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
//
