LOCUS       sequence-337118-        3585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag human PLD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-hPLD4
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        39..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     619..643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          732..828
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             844..861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             889..918
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             919..948
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             955..966
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2352..2486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2376..2395)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2430..2449
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2529..3117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2609..2628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3147..3521)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3522..3585)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtcgcg aggccatatg ggttaaccca tggccgttga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgtcgagc
      661 cgagtggttg tgcctccata gaagccacca tgacccgcct gaccgtgctg gccctgctgg
      721 ccggcctgct ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      781 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc cagcagccgc
      841 gcccatcatc atcatcatca tggtccgcta gttgacgtcg ccagcaatga acaaaaactc
      901 atctcagaag aggatctggc tagtatgact ggtggacagc aaatgggtcg ggatatcgaa
      961 ggtcgtgggc tagctcaagt gccccgtcct cccacctggg gccaggtgca gcccaaggac
     1021 gtgcccaggt cctgggagca tggctccagc ccagcttggg agcccctgga agcagaggcc
     1081 aggcagcaga gggactcctg ccagcttgtc cttgtggaaa gcatccccca ggacctgcca
     1141 tctgcagccg gcagcccctc tgcccagcct ctgggccagg cctggctgca gctgctggac
     1201 actgcccagg agagcgtcca cgtggcttca tactactggt ccctcacagg gcctgacatc
     1261 ggggtcaacg actcgtcttc ccagctggga gaggctcttc tgcagaagct gcagcagctg
     1321 ctgggcagga acatttccct ggctgtggcc accagcagcc cgacactggc caggacatcc
     1381 accgacctgc aggttctggc tgcccgaggt gcccatgtac gacaggtgcc catggggcgg
     1441 ctcaccaggg gtgttttgca ctccaaattc tgggttgtgg atggacggca catatacatg
     1501 ggcagtgcca acatggactg gcggtctctg acgcaggtga aggagcttgg cgctgtcatc
     1561 tataactgca gccacctggc ccaagacctg gagaagacct tccagaccta ctgggtactg
     1621 ggggtgccca aggctgtcct ccccaaaacc tggcctcaga acttctcatc tcacttcaac
     1681 cgtttccagc ccttccacgg cctctttgat ggggtgccca ccactgccta cttctcagcg
     1741 tcgccaccag cactctgtcc ccagggccgc acccgggacc tggaggcgct gctggcggtg
     1801 atggggagcg cccaggagtt catctatgcc tccgtgatgg agtatttccc caccacgcgc
     1861 ttcagccacc ccccgaggta ctggccggtg ctggacaacg cgctgcgggc ggcagccttc
     1921 ggcaagggcg tgcgcgtgcg cctgctggtc ggctgcggac tcaacacgga ccccaccatg
     1981 ttcccctacc tgcggtccct gcaggcgctc agcaaccccg cggccaacgt ctctgtggac
     2041 gtgaaagtct tcatcgtgcc ggtggggaac cattccaaca tcccattcag cagggtgaac
     2101 cacagcaagt tcatggtcac ggagaaggca gcctacatag gcacctccaa ctggtcggag
     2161 gattacttca gcagcacggc gggggtgggc ttggtggtca cccagagccc tggcgcgcag
     2221 cccgcggggg ccacggtgca ggagcagctg cggcagctct ttgagcggga ctggagttcg
     2281 cgctacgccg tcggcctgga cggacaggct ccgggccagg actgcgtttg gcagggctaa
     2341 atctagaagc tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     2401 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2461 ccattataag ctgcaataaa caagttggat atcctcgaga ctagtgggcc cgtttaaaca
     2521 catgtgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2941 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3121 gagaagatcc tttgatcttt tctacgtcag tcctgctcct cggccacgaa gtgcacgcag
     3181 ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc
     3241 atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac
     3301 agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc
     3361 tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc
     3421 acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg
     3481 cgcgcggtga gcaccggaac ggcactggtc aacttggcca tactcttcct ttttcaatat
     3541 tattgaagca tttatcaggg ttattgtctc atgagcggat acata
//
