LOCUS       sequence-337119-        3492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag mouse PLD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-mPLD3
FEATURES             Location/Qualifiers
     source          1..3492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        39..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     619..643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          732..828
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             844..861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             889..918
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             919..948
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             955..966
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2259..2393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2283..2302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2337..2356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2436..3024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2516..2535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3054..3428)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3429..3492)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgtcgcg aggccatatg ggttaaccca tggccgttga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgtcgagc
      661 cgagtggttg tgcctccata gaagccacca tgacccgcct gaccgtgctg gccctgctgg
      721 ccggcctgct ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      781 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc cagcagccgc
      841 gcccatcatc atcatcatca tggtccgcta gttgacgtcg ccagcaatga acaaaaactc
      901 atctcagaag aggatctggc tagtatgact ggtggacagc aaatgggtcg ggatatcgaa
      961 ggtcgtgggg acttacatct atttggacca aatcagcgcc cagccccctg ctatgacccc
     1021 tgcgaggcgg tgctggtgga gagcattcct gagggtctgg agtttcccaa tgccaccacg
     1081 agcaacccct ccactagcca ggcctggttg ggcctccttg ccggtgctca cagcagcctg
     1141 gacatcgcat ccttctactg gaccctcaca aataatgaca cccacacgca agagccctct
     1201 gcccagcagg gtgaagaggt tcttcagcag cttcaggcac tggcacctcg aggtgtaaag
     1261 gttcgcatcg ctgtgagcaa acccaatggg cctctggctg atctgcagtc tctgctacag
     1321 agtggtgccc aggtccgcat ggtggacatg cagaagctga ctcatggtgt cctgcacacc
     1381 aagttctggg tggtggatca gacccacttc tacctgggca gtgccaacat ggactggcgg
     1441 tcgctgaccc aggtcaagga gctgggcgtg gtcatgtaca actgcagctg cctcgcccgg
     1501 gacctcacca agatctttga agcctattgg ttcctgggcc aggcaggtag ctccatccca
     1561 tcaacctggc cgcggtcctt tgacacccga tataatcaag aaacaccaat ggagatctgc
     1621 ctcaatggca ccccagctct ggcctaccta gcgagtgcac ccccaccact gtgtccaagt
     1681 ggccgcaccc cagacctgaa ggctctgctc aacgtggtgg acagtgcccg aagcttcatc
     1741 tacattgcgg ttatgaacta cctgcccacc atggagttct ctcatccacg caggttctgg
     1801 ccagcgatcg atgatgggct gagacgggct gcgtatgaac gaggcgtcaa agtgcgcctg
     1861 ctcatcagct gctggggaca ctctgatcca tcgatgcggt ccttcctgct ctccctggct
     1921 gcacttcatg acaaccatac tcactccgac atccaggtga aactgtttgt ggtccctacg
     1981 gatgagtccc aggcccgaat cccctatgcc cgtgtcaacc acaacaagta catggtgact
     2041 gaacgtgcct catacattgg aacctccaac tggtctggaa gctacttcac ggagacagca
     2101 ggcacctccc tgctggtgac acagaacggg catggtggct tgcgcagtca gctggaggct
     2161 gttttcctga gagactggga atccccatac agccacgatc tcgacacctc agccaacagt
     2221 gtgggcaatg cctgccgcct gctttaaatc tagaagctga tccagacatg ataagataca
     2281 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2341 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggatatc
     2401 ctcgagacta gtgggcccgt ttaaacacat gtgtttttcc ataggctccg cccccctgac
     2461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3001 acgcgcagaa aaaaaggatc tcaagaagag aagatccttt gatcttttct acgtcagtcc
     3061 tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
     3121 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
     3181 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
     3241 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
     3301 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
     3361 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
     3421 ttggccatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3481 agcggataca ta
//
