LOCUS       sequence-33712-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB541
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(170..256)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(272..366)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
       61 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
      121 gagtcagtga gcgaggaagc ggaatatatc cctaggtcta gggcggcgga tttgtcctac
      181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      241 cctttcgttt tatttgatgc ctctagcttg gattctcacc aataaaaaac gcccggcggc
      301 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      361 ggagtccaag ctcagctaat taagcttaca tcatgccgcc catgccaccc atgccgccca
      421 taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta accatgcatt
      481 cggtggtgat catcaggcca gccacagaag ctgcgtactg cagagcagaa cgagttactt
      541 tggttggatc caggataccc atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt
      601 aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt tcttcgccgc
      661 agttcaatac gatctgacgc agcggagctt ccattgcacg cagtgcaact ttgataccca
      721 cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg cggatcagcg
      781 caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg tgcagggcat
      841 cttcaa
//
