LOCUS       sequence-337166-        9692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of 
            destabilized eGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Mendez-Mancilla A, Wessels HH, Legut M, Kadina A, Mabuchi M, Walker 
            J, Robb GB, Holden K, Sanjana NE
  TITLE     Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in 
            human cells.
  JOURNAL   Cell Chem Biol. 2022 Feb 17;29(2):321-327.e4. doi: 
            10.1016/j.chembiol.2021.07.011. Epub 2021 Aug 2.
  PUBMED    34343484
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2021.07.011"; journalName: "Cell Chem Biol"; 
            date: "2022-02-17- 17"; volume: "29"; issue: "2"; pages: 
            "321-327.e4"
COMMENT     Sequence Label: pLenti-TRS-Leader-SARS-CoV-2-EGFP
FEATURES             Location/Qualifiers
     source          1..9692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    77..198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(88..107)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     142..161
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             276..509
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    581..1169
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             687..698
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1096..1116
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1194..1241)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(1242..1361,1362..1367,1368..2078,2079..2081,
                     2082..2084))
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1410..1431)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1759..1778
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2018..2039
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2081..2090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2185..3363)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2194..3132
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2217..2237)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3501..3618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3689..4708)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     promoter        complement(4730..4926)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(4738..4948)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4744..4879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(5179..5223)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5408..5641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6134..6259
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6306..6486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6487..6713)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6741..6761)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6741..6759)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6780..6796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6780..6796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6793..6815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6804..6820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6828..6834,6835..6852,6853..6858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6873..6894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7011..7028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7182..7770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7262..7281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7941..8732,8733..8801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8564..8583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8802..8906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8932..9387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9069..9090)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9281..9300
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9514..9536
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9528..9545
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9529..9545
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9555..9574
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9555..9573
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(9594..9692,1..37)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag
       61 ctagagccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      121 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      181 agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata
      241 ctgaataata agatgacatg aactactata cgtactgcta gagattttcc acactgacta
      301 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
      361 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
      421 ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg
      481 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
      541 atctacagct gccttgtaag tcattggtct taaagtcgac gcggggaggc ggcccaaagg
      601 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
      661 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
      721 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
      781 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
      841 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
      901 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
      961 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     1021 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     1081 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     1141 tcacaaattt tgtaatccag aggttgattg tcgacttaac gcgtggatcc tcatttcttc
     1201 ttcttagcct gtccagcctt ctttgtggcg gcaggtcgct tcacattgat cctagcagaa
     1261 gcacaggctg cagggtgacg gtccatcccg ctctcctggg cacaagacat gggcagcgtg
     1321 ccatcatcct gctcctccac ctccggcggg aagccatggc taagcttctt gtacagctcg
     1381 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     1441 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     1501 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     1561 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     1621 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     1681 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     1741 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     1801 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     1861 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     1921 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     1981 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     2041 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc atcgatgcta
     2101 gcgttcgttt agagaacaga tctacaagag atcgaaagtt ggttggtttg ttacctggga
     2161 aggtataaac ctttaatgct agcctcacga cacctgaaat ggaagaaaaa aactttgaac
     2221 cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga
     2281 gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa
     2341 ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa
     2401 ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga
     2461 ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg
     2521 cgtcctccat tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc
     2581 aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag
     2641 gccaggcacc agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg
     2701 cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc
     2761 cgcggcccca aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc
     2821 ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc
     2881 gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc
     2941 aggccaggcc tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact
     3001 ctcccaccca cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga
     3061 agtaattcaa ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca
     3121 cacggcactt acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac
     3181 tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac
     3241 atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc
     3301 tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga
     3361 gccaattccc actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt
     3421 ttaaaacttt atccatcttt gcacctgcag gcctcgagcc ggcgccaaag tggatctctg
     3481 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     3541 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     3601 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctcaagat ctagaattcg
     3661 ttaacggcgc gcctcattac tgcagatcct ttgccctagg acgagtgctg gggcgtctgt
     3721 ttccactatc ggcgagcact tccacacagc catcggtcca gacggcagcg cttctgcggg
     3781 cgatttgtgt acgcccgaca gtcccggctc cggaccggac gattgcgtcg cagcgaccct
     3841 gagcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     3901 caatgcggag catataagcc cggagccgag gagatcctgc aagctcggga tgcctccgct
     3961 cgaagtagcg ggtctgctgc tccatacaag ccaaccaggg cctccagaag aagatgttgg
     4021 cgacctcgta ttgggaatcc ccgaacatgg cctcgctcca gtcaatgacg gctgttatgc
     4081 ggccattgtc ggtcaggaca ttgttggagc cgaaatcggc gtgcacgagg tgccggactt
     4141 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgggcgacg gaggcactga
     4201 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaata gcgcagatga
     4261 aatcgcgcca tgtagtgtat tgaccgattc cttgaggtcc gaatgggccg aacccgctgg
     4321 tctggctaag atcggcggca gcgatggcat ccatggcctc ggcgacgggc tggagaacag
     4381 cgggcagttc ggtttcaggc aggtcttgca aggtgacacc ctgtgcacgg cgggagatgc
     4441 aataggtcag gctctcgctg aactccccaa tgtcaagcac ttcgggaata gggagagcgg
     4501 cggatgcaaa gtgccgataa acatagcgat ctttgtagaa accatcggcg cagctattca
     4561 cccgcaggac atatccacgc cctcccacat cgaagctgaa agcccgagat tcttcgccct
     4621 cggagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     4681 aggtagcggt gagttcaggc tttttcatgg tggtacctct agaaagcttt ttgcaaaagc
     4741 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     4801 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     4861 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     4921 gacgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     4981 ctgactaatt gagatgcaat gcatgggtcc cctcggggtt gggaggtggg tctgaaacga
     5041 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     5101 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     5161 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     5221 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     5281 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     5341 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     5401 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     5461 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     5521 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     5581 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     5641 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
     5701 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
     5761 catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt
     5821 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
     5881 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
     5941 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
     6001 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
     6061 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
     6121 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
     6181 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
     6241 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
     6301 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     6361 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     6421 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     6481 agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc
     6541 agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta
     6601 ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg
     6661 ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct
     6721 tgcagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     6781 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6841 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6901 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6961 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7021 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7081 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7141 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7201 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7261 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7321 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7381 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7441 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7501 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7561 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7621 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7681 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7741 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7801 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7861 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7921 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7981 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8041 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8101 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8161 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8221 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8281 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8341 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8401 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8461 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8521 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8581 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8641 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8701 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8761 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8821 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8881 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     8941 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     9001 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     9061 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     9121 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     9181 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     9241 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     9301 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     9361 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     9421 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     9481 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     9541 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacgtcc ctaggcctcc
     9601 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     9661 ataaataaaa aaaattagtc agccatgggg cg
//
