LOCUS       sequence-337176-       13611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express sygl-1 fused to 24 copies of MS2 loops..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Lee C, Shin H, Kimble J
  TITLE     Dynamics of Notch-Dependent Transcriptional Bursting in Its Native 
            Context.
  JOURNAL   Dev Cell. 2019 Aug 19;50(4):426-435.e4. doi: 
            10.1016/j.devcel.2019.07.001. Epub 2019 Aug 1.
  PUBMED    31378588
REFERENCE   2  (bases 1 to 13611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.07.001"; journalName: "Dev Cell"; date: 
            "2019-08-19- 19"; volume: "50"; issue: "4"; pages: "426-435.e4"
COMMENT     Sequence Label: pJK2014
FEATURES             Location/Qualifiers
     source          1..13611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5328..5339)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7157..7228
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             8217..8234
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             12110..12121
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 accccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgcactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc ccagtcacga
     2341 cgtcacagca cgtgtgtttt tgaacacggc gatatgtatc tgtagatcat ccccaatttt
     2401 caacaatgaa tctaaatact gaactttttg tgtaatcttt agtgcagaaa aatgaccaaa
     2461 atcagcgatt ttccgattat acccaaaata tctcagctca atgagcacct ataaaaatgt
     2521 aaaaataaag aaagtagtcg aaccatattt tgtaaaggct aaaaaactga ataaattttt
     2581 atccatccag cgcggcgtta caatccgtgg cccatgtcac gcacggcttt caaagaaaaa
     2641 aaaacagtct gtttgcgcga catcgatctg gcaaaaatcc taaaaacgac tatgcgtggc
     2701 tcactttata ttaatatgaa aaatacaaac ttggtggtgc aggtgctgca gaggccatcg
     2761 atgaagaagt ggaggccttg ttgtcgatct acaaaattgt atggttgttt gagattttcc
     2821 ttgaaacgct ctcaatcttg gatatgatat aaattctgga taagatatga tggatatgat
     2881 ctatgtttga tagagtgcat ttgaaaggat aacggccctg cagacatttt tgaaaaaata
     2941 acaaacattt aattttctca ctcgtttagg ctattcccct atttttgata ttccttcgca
     3001 catatgaaaa ctactttttt tcgaaactgt taactccaga atttataaat ctatagccct
     3061 tacttgatta tttattatca tggttactca cctcatgtct ctttctcttt tcaagaagtc
     3121 ttcgttcata tctgttgtct ctttcctcct tttgaatctc tgctttgctc ttcgccattg
     3181 tttcctgaaa ataatgtaac ttgaattgtg taatatactt ttttaatttg aatttggctt
     3241 gtaacgcgga atcactacgt gcgggatcat ttcttactag aaaacccaga aaatgccata
     3301 tttcacttat ctcggggtca tttctaatta gaaaagctac aaaaaccatt tctaattacg
     3361 gtgactttag aactggtcga aatagctaat tagaaatggg tttttgctaa ttagaggtga
     3421 ctctatgtta tctgagtaag cctcgtttgt atgtaaactc acagtactaa taaatgcaag
     3481 acacccgggt ttgtctagat atgaaataat tgaaatatca attctgacag acaataatgg
     3541 taatcttgat aaggagttcc acgcccagga gaacacgtta gttttcttgt ttttgattgc
     3601 gtgcgttatt ttggagaaaa actcgatttt ttacaaaata attttttgaa aggaacactg
     3661 ttcaataagt tttgtctttt ttctcagttg tgatacggtt ttttattctt ttttgtagtt
     3721 atacagaaga ccgttacgaa acagactgat atcgaaacaa acactgatct tacttgcact
     3781 tataatacga ctcatgatga acggtttctt gactggcttt cgtccgtgta gtcctgcttg
     3841 ctgtaaacgt cgacgaacag ttcgtttact tggtacaggt tcatttggag aacttataat
     3901 catttgaata tccgtggcgg tcctatgcgg atcttctctt gctgatcgga ggatgttgcg
     3961 atccatccta tgggttgtca ctcgaggcct gccgggcgag attctcaaag cgacggattt
     4021 ctaaacattt attattaaaa aataatattt ttcgaactca cctcagtttg gtacttcttg
     4081 attactttcc aaatagtcga cggagaacgt tgaatttgca gcgcgagcat tttcgtgggt
     4141 attccttgtt cgaagccagc tacaatggct ttcttgacgt ccaaggaaag atttttacac
     4201 ccaacagatt ttaccatacc ttaaaaataa tttatcaacc aaacaaatcc agagcaacaa
     4261 acagagtgcg ttttattgat aattcaaaaa aaataatgaa aattggaaaa acgaaaaaat
     4321 caaaaaattg aaataaaatt cagaaaaaat cgataatcat gtaatgtttg gtccgcaaat
     4381 ttgcgtaaca tttcgcttta tgcacacgga aaaattcaag tcaaatggat gcttgagaaa
     4441 aaagttacac acaaaaaacc aaaagtggat atctttttgg ccagcactgt acgttgaccg
     4501 cagttaacat aaccgtattt tctccctcat tttctcctat tttcccccta atttcccctt
     4561 tttttctttt tttctaggag tcgtcgccgc tttgctgaaa tcttgcttaa atctataaaa
     4621 acagaccaca atttagtgat tttgtccgtt ttgcattttc agtaggtgac cctagtttgg
     4681 aaattttgaa ttttaccatt tgaagaggta ttaggttggg agttggtggg tgataaggga
     4741 gaggtacttt gttatactgt attattagta ttattagtaa gagtactgta ggatttgtgt
     4801 agtttgggga aatttgaatt ttttgctatt attaggatag ggctggtttt caggcgagtt
     4861 acacgggttt aaagttgatg gcctagaaat cttaaggcca ccaattttta gaatttatcg
     4921 attttagtag atttgagaca tgttttcgat tttgtctgtt tttcggaatt tgaagaaaat
     4981 ttccctctga aaaaatcgat aatttttgaa ttccggagtt gttagtcgaa atctctcgaa
     5041 tttgcgctgt tttctgtcga ttctcagaga ttaagggtcc aataaaatta tgttggcatg
     5101 ttttttatta gcagtgtgtt ctgccgaaat ttaatgaaaa atagtttttc cggctttttt
     5161 taaaataata tttgtgtttt tttcgacgat aattaccaat tttctttgaa ttttcaaggt
     5221 tttttctgta gtttttactt gtttatcttg tatttccaat ttatttcagt atttcctcag
     5281 tggcgtcttc agtggatttc ttttttcgcg aagaagatcc cgaaaatttg caattcccac
     5341 taaaaggact actgtagtca atacattgtg tgacaccgcc acgcgaaagc gcacagtttc
     5401 ccacgctttt gccgtgggaa tttccaattt tcccacggtg attttagcat ataagaggag
     5461 aaaaatgggt caatttccat ttatttttcg acgaaaatgt aagttttttc gattttttat
     5521 caaattttgt tggttttctt gagaatttag tgaaaaatat tgacttttag attaaatttt
     5581 aaattttgtc ggaaattttc aaattttttg cataacttcc atggagttag agtttaattg
     5641 aataagtttt tgttcaaaaa ataaaaaaat tatgcccaaa atttatttat cactaaaaca
     5701 aattttcgaa aaaaaatttt ttttttgatc tacccgccga ttttctaatt tttctccttt
     5761 ttttcagcga tcatcgaacc attgtcatca cgccacgtgc atagcggccg cgaattcgcc
     5821 cttcgcggga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag
     5881 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc
     5941 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca
     6001 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta
     6061 ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat
     6121 cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag
     6181 cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc
     6241 cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag
     6301 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg
     6361 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag
     6421 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc
     6481 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca
     6541 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta
     6601 ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat
     6661 cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag
     6721 cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc
     6781 cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag
     6841 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg
     6901 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag
     6961 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc
     7021 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca
     7081 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatctc gcgaagggcg
     7141 aattcgttta aacatggatt acaaggatga cgatgacaag gactacaaag acgacgacga
     7201 caaggattat aaggatgatg atgataagcc attccattat ccaaaactct acatggatca
     7261 ccgcggaaac atgtccacct catcgtctct gacgtcatcg acaaccgcca cgtcatcatc
     7321 gtcaaccgga ggcgtccgca agcagattct cacgttgaag ccaaagcaac cgaatggtta
     7381 tgtgcaacac ttggagatca aggcaaaggt atagattttt gttttaaaaa tcattttttt
     7441 gaaccctaaa attgattttt tctctttaaa aagttggatt tttccaataa aactgtttaa
     7501 aaaaagcttt caaaaccgaa aattcgtgct cagcaccctc gaaaacccaa aaatcccgca
     7561 aaaatcgata attttctctt aattttgaaa aattggtcga gtggcgtagt gggattttcc
     7621 acaacctgcc accaatcaca agaacagtgt tcgagtcccc actgtggcaa cttttttttg
     7681 tttaagcttt gtttaagctg tttaagcgaa agctttaaca catgcggaca tcaaaaattt
     7741 gactgaaatt tcagattttt cccagttttt actcaaaaaa tgtaattttt gagctttttt
     7801 aaccgaattt tcggcttaaa gcctgatttt cgcagtccca agccctttta gaacaatgct
     7861 tcaaaaaaca aaaagatttt gccacagtgg ggactcgaac cccggtctcg tggcagtggc
     7921 agccgacgaa aatcccactg agccatttgg gcactttttt gaaaaatcct caaaatagcg
     7981 gaagttatcg atttttaagc caaacaaata gctctgggtt cgatttttcc attaaaaatt
     8041 atcgattttt tcaggaatca agccaatctt cggtgatttc cggcggtgtc ccggttccgg
     8101 tgaccctgtt ggagttgaag aagaaggatc gtagcgagaa gaatgtggtg accaacctga
     8161 agtatcgtaa gccaacggcg aatcatcatc tcgattcggt gcattcttcg tcggagcacc
     8221 atcaccacca ccatctgagc ctgagctctt tgacgcgccg cccgggatct ccgtcctcgt
     8281 ccggttacga gactgactcg ctcgtcgatc aggagaattg cgacgttttt tcggtaaatt
     8341 taaaaaattt caaatttgag aaaatccaga aaaaaaaacg gaaattttga accaaaattg
     8401 actttttttt ttttgctcaa tttttccact aaaagtgaac tttttcatct ttaaactact
     8461 taaaaatcag ccttcaacaa tttttctcat caaattccgg tttctctttc caaaaatcca
     8521 gatttctgcg aaaaatgtcc atatggcgca gtgggatttc ccatgtgcct gccaatttta
     8581 agacccgggt tcgtgtcccc actgtggcaa cttttttttt cgaagtgcga ttaggttaat
     8641 tttagtgtcc tgagctcgaa aatctttgat aaatttattt ttcctcgcgg aaaacatggg
     8701 aaaaaatttg aaaaacttcg aaaatcccta aattttcgat taataccgat tccccatgac
     8761 atctgagcac actgtaaaac ctaatcgcaa cgaaacaaaa aattttgata cgacggggag
     8821 tcgaaccccg gtcttgagat tggaagccga cgaaaatccc actgcgccat ttggacattt
     8881 tgctgcaaac tgccagaaat ctaaatttta atgctttttt catagtttca agtgttcaaa
     8941 aagcagtttt cagtagcttt tttttatttt ttcaattaaa aaattcaaat ttccctccat
     9001 tttcagagtc tcttcaactc gtcgctcagc taccaatcgg ctctcgacca tcatcatcag
     9061 tttcacgagc tcgacatgct tcactcgcat ccgatttggt ctccacagtt accgaacaac
     9121 aacacttcac tgatgatggg ctccaacagc tattatttgc agtagagcgt acttgctctt
     9181 ttaaattttc taatcccccc ccccccctaa aatcccaccc cacgcctaat tctttaacta
     9241 ataagaatta tcaatttttt tggttttctt tctgtgtaat aaactccgcc cccctatttc
     9301 tagccccacc cctttatctc tcatttttct aataattctt tgttcaatcc catcattctt
     9361 accccaactt ctgtttcagc tccccccaat tcctcctaat tattctaatt tattacaagc
     9421 tcctcccatc tccaaccctc cgtgcttgcg aaaataaacg ttttataagt aaaaaattgt
     9481 gttttttttc tgaaattttt cgaaaaaata ggttttcgac tcgggaagta ctttactagt
     9541 tatggcaaat cgaatgaaaa gctgaaaaaa atttttgcca cagcgcgagc ttgaaccccg
     9601 gtcttgtgat tggtgggcga cgagaatctc actgcgccaa tcggacacaa aagtgctgta
     9661 ataatcgatt ttcggttgaa attactgttt tttaccactt tctagacgat ttcctttctt
     9721 ctctgtgtat ttttttttta atttttctag taggaaaatt gaattttaag cgattaataa
     9781 agaaaaaaaa tcaattttca agagagattt ttggaattca agcattttta ttaaatttaa
     9841 aatctttttt cttaatttct acctatttta gtttatttct cccatttatt cccttttgtt
     9901 tccatccaaa acccacaatt tccggccggt gtgcggtgga gcgcgtttgc acacttttat
     9961 tccagctagc tgcttgggca gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa
    10021 ttcctgcagg atatctggat ccacgaagct tcccatggtg acgtcaccgg ttctagatac
    10081 ctaggtgagc tctggtaccc tctagtcaag gccttaagtg agtcgtatta cgtagcccgg
    10141 gcctagttct agacattctc taatgaaaaa atctttcagt tgaaattgaa aatgagttaa
    10201 agttggagtt tttattgaaa acagatttcc gtgtgattag tgtttttagc gagtgtgaca
    10261 ggacagcgaa aaaatataga aacaaggggg gaactgaaaa gcttaggaat gcattgaaca
    10321 tgagaagggg aaggggaagg aacaaactag acaggaatta ttggaattta atcacatttg
    10381 gagttttttt tctattcgac agaataatta tccagaacat ttttgtatta aatatttatg
    10441 catcatatga gtagtcggct ttgttgtgca tgacgagttt gttatcgacg aaatagaagc
    10501 tgtcagaacg agtctcgttt ggattgttga tcatgtcgtc cactgaaaaa gagattagtc
    10561 ttttgaattg tactttttag aataatgact cactgagttg ttgagagagt tgagggaact
    10621 catagatatg ttcacagttg tttcgtgaat tcggaataca gaatccgaat tcaaagtcaa
    10681 aacacttcag aaggcgatct ttgaagaagt gacgttcgat cattcggaaa tgatggattg
    10741 ggatgtctcc tactttgaat tccacagttg ctccgaccgt tttgagtttc aaaaagtttg
    10801 gagcaaatct ataacgcacg tatcttgccg actcctgtgg cgactcatca ttctcctgat
    10861 cgttctccgg tttggcgatc tcgaaaagca cttgctcagt gtccagatca cggatttgga
    10921 acttggtgaa ctcgatgtta tagatgttcg cagatgggga gcataagaat cctaaattta
    10981 tgttttaaac tgaaatccaa agggagcaag ataccttgag tgattcccgg aagtgctaaa
    11041 acgtcgttcg gagtgatttg agctttcttc gcaagctccg attccgttgt gattccttgt
    11101 tcggtgcttg gtggtggccg tggcatctgg aaatatggaa aagttcaaca aaaagaaaag
    11161 agaaaagaat gaaatcggat atcaagagtt agttgagcgg tttctctagt tttctgagtc
    11221 tcacctgcga cgggaaggtc gccgagccgg gtggaatcga tcgttgttgc tcggctttca
    11281 tatcggtttg gttggaagcg gctgaaaacg gaaagaagtg gaagaaggaa aagagtgtgg
    11341 tgtgacagga aaatggtaat tagagggtgc caaataacca gctatatttt gttttttttt
    11401 gaaaacattt ttaaaaagaa aaatacgata atgatatcag atggatttcc ggaaaactgg
    11461 tatgaaaaat ttcaaccttt ttgagtacat gtaatcaaaa tacactttgt aaattatcat
    11521 ttttattgaa actccaccat ttttctattt ataacgctaa taatttgaaa aagaaaccta
    11581 ttgcgaaccg cggggtgaat cccaaaaacg aatgcgtttt ggtggagtga ttgattcgaa
    11641 tcgaagaaga aaaagaagaa gacgtggaat agagagctca ctcttaaccg agcagcacac
    11701 accgacagaa aaaaaaatga aatgaatgag ggtcttcttc ttcttcttct tcgaatgatt
    11761 gacagaaatg ggaaaaagag gaagattgag aagggaaaaa ggaaggagaa aagaagcaga
    11821 agaagacgtc agagaggaga ggaacgagcg gaaaagcagc gggcgcaagt catagaagta
    11881 gcagagctgg ggagaagaag acactatcca agaaaggaat gacgagagag tatgcaaagg
    11941 ggtatagggt gcagacagaa taggaacaga ataacagatg atgagccaag aagagttgaa
    12001 aagggcgatg aatttgtcat gtaacttaat ttgggtcaat ttgagcatga tgaattgaaa
    12061 tcatcccttg ttgggagtta ataaccggtt tgttatcaga aaccctgtaa tagaagggcg
    12121 ccctaacttt gagccaattc atcccggttt ctgtcaaata tatcaaaaag tggtcaactg
    12181 acaaattgtt tttgatatta taataaacat tttatccgtt aacaattttc gaatactttt
    12241 tacaaggact tggataaatt ggctcaaaga cgaagtgagt ttgctaccat catggaatca
    12301 ggaggacatt tcgacaatgt catataatgt ttgaaaaatg attttaacaa gggtctcatt
    12361 cgagataagc aatgctctga gcaagtggct cgtcgtcttc aagaatctct tgttgatatt
    12421 catttggatc ctgttttgca cgaagcatgt gccatggata ttcagagttt ttgcaaaaaa
    12481 gttccgccag gtcaaacaat ctttttaatt tttatatgat acttcgggtg tatttttctt
    12541 ctttttcctg ttcattttcg cttttttctc acttttttgt aacaaaatct ctcacttttc
    12601 tgtaagtctt aacaaaagtt caattggctc gatcaagtaa aatttcggcc gtatgaacgt
    12661 tttcaaaggt tttcattttt ttttaagttc attcacaata tcatgaatct ctcgtccttt
    12721 ggtttccggt aagtagcgaa ataatataag catacttata aatgaaggaa ttgaatatag
    12781 aataaggaaa gcataactac caataacgga atatagaggt aatactgtaa atgtactgat
    12841 aaccaccatt acagtattca ttgaatatgc cacagattga gcaatactac gatgtttttg
    12901 aggtaccagt tcagaggata taaaccacga aattggacca actccagttc tgaaatttca
    12961 ttattcgatt aaatcaaaat atatttgaaa aacctcaccc ataagtataa ccgtagatga
    13021 tcaatgcaac taggcatccg tatttcatcg ggtcaatcag aatacgtatt tttgcgaaga
    13081 atacgaatgc aattagagct aaattattga gaaatgtgaa gagaaggaga agcttgcgtc
    13141 gaccaactct gtaaacttat tcatacaatt ttgtcttcaa attttgaacg tttaaaacta
    13201 tcttattcaa tgagttagcg ggtttttata gtgagttagt tcacgattat ctaataaaac
    13261 tatgtccaaa catcctaact gcgaccgtat tctcactaac tcgtcttaaa aacgttgaga
    13321 ttattttaaa cccttcttca gtacaactac agtactgatg cacaaataca ataataataa
    13381 tacattcaga catactctga acaatgtaac ttgtttttgt tatactgtgg taacatttat
    13441 tgaaatgaaa tataacctta cttttcaatc acaattccac cagtaattgt tccagaaaca
    13501 taagctaaac ccattgcagt tgtactccat tcagcaattt ctgactcaac atttgcattt
    13561 tctagaaaga aagtagagga taaaagaaga gcccataatc cgacagggcc c
//
