LOCUS       sequence-33718-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB572
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     157..175
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             256..267
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
       61 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
      121 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      181 cgtcttcacc tcgagaaaat ttatcaaaaa gagtgttgac ttgtgagcgg ataacaattt
      241 cacacagaat tcattaaaga ggagaaagga tccatatgcg taactttgat ttatccccgc
      301 tttaccgttc tgctattgga tttgaccgtt tgtttaacca cttagaaaac aaccagagcc
      361 agagtaatgg cggctaccct ccgtataacg ttgaactggt agacgaaaac cattaccgca
      421 ttgctatcgc tgtggctggt tttgctgaga gcgaactgga aattaccgcc caggataatc
      481 tgctggtggt gaaaggtgct cacgccgacg aacaaaaaga gcgcacctat ctgtaccagg
      541 gcatcgctga acgcaacttt gaacgcaaat tccagttagc tgagaacatt catgttcgtg
      601 gtgctaacct ggtaaatggt ttgctgtata tcgatctcga acgcgtgatt ccggaagcga
      661 aaaaaccgcg ccgtatcgaa atcaactaat tccctaaggc cgcctggcgc ggcctgacat
      721 ctccatgctc gccgtcaggg agcatatgcg aatcttcgga tttgcaggta cttactcgct
      781 tcttagaa
//
