LOCUS       sequence-337182-        4697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C. elegans mex-5 promoter (germline) driven dual fluorescent 
            reporter for boxB tethering (eGFP) with boxB negative control 
            (mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Aoki ST, Lynch TR, Crittenden SL, Bingman CA, Wickens M, Kimble J
  TITLE     C. elegans germ granules require both assembly and localized 
            regulators for mRNA repression.
  JOURNAL   Nat Commun. 2021 Feb 12;12(1):996. doi: 10.1038/s41467-021-21278-1.
  PUBMED    33579952
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-12"; volume: "12(1):996. doi"; pages: " 
            10.1038/s41467-021-21278-1"
COMMENT     Sequence Label: pJK2067
FEATURES             Location/Qualifiers
     source          1..4697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(657..685)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1433..1453
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(1495..1519)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1515..1556
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     protein_bind    2112..2130
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2156..2174
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    2180..2198
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             3361..3402
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4264..4275
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attctttttt gtagttatac agaagaccgt tacgaaacag actgatatcg aaacaaacac
       61 tgatcttact tgcacttata atacgactca ctgcaagaaa atacattttc gactgatttt
      121 acggttttca caacggcaaa atatcagttt ttaaaaaatt aaaccataaa acaaataata
      181 taacccaatt tttacatcaa accacaagaa aaaaatacat ttgggcccac ggataaagaa
      241 attaaaaaaa tacatttttt aaaggcgcac cgaattaaaa ttcatttggg tcttaccgcg
      301 tataccgtac tccgtttgtt tgatcatttt tgtcagcgct ggcggttgtt ttttcatttc
      361 atttctgctt caaagacgtt ttctcgaata atttttcgtt tattctcttt tttaaaatta
      421 atttctagcc gtaaatgtta taaattcacc catttaacgc aaatttcatg gtaatctcat
      481 ggaaaaatgc agtttctttg ttaaagaaag cttaaatagc aaaaattccc cgactttccc
      541 caaaatcctg ctcgattttc cgttttctca ttgtattctc tcttaattaa ttttatcgat
      601 aatcaattga atgtttcaga cagagaatga gtaaaggaga agaacttttc actggagttg
      661 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      721 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      781 aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct gattatttaa
      841 attttcagcc aacacttgtc actactttct gttatggtgt tcaatgcttc tcgagatacc
      901 cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      961 aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt aaacagttcg
     1021 gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt gaaggtgata
     1081 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1141 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1201 agaatggaat caaagttgta agtttaaaca tgattttact aactaactaa tctgatttaa
     1261 attttcagaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1321 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1381 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1441 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaaacccag
     1501 ctttcttgta caaatccgga ttcgccaacg agcttggacc acgccttatg ggaaagcagt
     1561 ccggaatgcc accaaagcca tctgccaagg gagccaagaa ggccgccaag accgtcgttg
     1621 ccaagccaaa ggacggaaag aagagacgtc atgcccgcaa ggaatcgtac tccgtctaca
     1681 tctaccgtgt tctcaagcaa gttcacccag acaccggagt ctcctccaag gccatgtcta
     1741 tcatgaactc cttcgtcaac gatgtattcg aacgcatcgc ttcggaagct tcccgtcttg
     1801 ctcattacaa caaacgctca acgatctcat cccgcgaaat tcaaaccgct gtccgtttga
     1861 ttctcccagg agaacttgcc aagcacgccg tgtctgaggg aaccaaggcc gtcaccaagt
     1921 acacttccag caagtctaga cttagccatg gcttcccgcc ggcggtggcg gcgcaggatg
     1981 atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac cctgcagcct
     2041 gtgcttctgc taggatcaat gtgtctagag gagaagaata aatgcaagat ccaactacta
     2101 aactgattcc tgggccctga agaagggccc ctcgactaag tccaactact aaactgggcc
     2161 ctgaagaagg gcccatatag ggccctgaag aagggcccta tcgaggatat tatctcgact
     2221 ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct
     2281 aatttatttg cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg
     2341 gatgtgactg atcagaatgc tatcaataaa ggttgtatat ttattcatct tattgaatct
     2401 gccatttcct cgtttttgcg agtttatata ccttccaatt ttctttctat tgtattttca
     2461 acttctaatt ttaattcagg gaaactgctt caacgcatca tggtctcaaa gggtgaagaa
     2521 gataacatgg caattattaa agagtttatg cgtttcaagg tgcatatgga gggatctgtc
     2581 aatgggcatg agtttgaaat tgaaggtgaa ggagaaggcc gaccatatga gggaacacaa
     2641 accgcaaaac taaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     2701 tttcaggtaa ctaaaggcgg accattacca ttcgcctggg acatcctctc tccacagttc
     2761 atgtatggaa gtaaagctta tgttaaacat ccggcagata taccagatta tttgaaactt
     2821 tcattcccgg agggttttaa gtgggaacgc gtaatgaatt ttgaagacgg aggagttgtt
     2881 acagtgacgc aagactcaag gtaagtttaa acagttcggt actaactaac catacatatt
     2941 taaattttca gcctccaaga tggagaattt atttataaag tcaaacttcg aggaacgaat
     3001 ttcccctcgg atggacctgt tatgcagaag aagactatgg gatgggaagc ttcaagtgaa
     3061 agaatgtacc ctgaagacgg tgctcttaag ggagagatta aacaacgtct taaattgaaa
     3121 gatggaggac attacgatgc tgaggtaagt ttaaacatga ttttactaac taactaatct
     3181 gatttaaatt ttcaggtgaa gacaacttac aaagccaaaa aaccagttca gctgccagga
     3241 gcgtacaatg ttaatattaa actggatatc acctcccaca acgaggatta cactatcgtt
     3301 gagcaatatg aaagagctga agggcggcac tcgacaggtg gcatggatga attgtataag
     3361 ggaaagccaa tcccaaaccc actcctcgga ctcgactcca ccggatccat gccaccaaag
     3421 ccatctgcca agggagccaa gaaggccgcc aagaccgtcg ttgccaagcc aaaggacgga
     3481 aagaagagac gtcatgcccg caaggaatcg tactccgtct acatctaccg tgttctcaag
     3541 caagttcacc cagacaccgg agtctcctcc aaggccatgt ctatcatgaa ctccttcgtc
     3601 aacgatgtat tcgaacgcat cgcttcggaa gcttcccgtc ttgctcatta caacaaacgc
     3661 tcaacgatct catcccgcga aattcaaacc gctgtccgtt tgattctccc aggagaactt
     3721 gccaagcacg ccgtgtctga gggaaccaag gccgtcacca agtacacttc cagcaagtct
     3781 agacttagcc atggcttccc gccggcggtg gcggcgcagg atgatggcac gctgcccatg
     3841 tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc
     3901 aatgtgtcta gaggagaaga ataaatgcaa gatccaacta ctaaactgat tcctaacggg
     3961 taaagtaggg caactcgact aagtccaact actaaactaa cgggtaaagt agggcaaata
     4021 taaacgggta aagtagggca atatcgagga tattatctcg actttcaagc attcccttct
     4081 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     4141 aatgttatta ttttatgact ttttatagtc actgaaaagt ttggatgtga ctgatcagaa
     4201 tgctatcctt ttttccagtg ttctcattat gtttcaatca ttaaaatatc ttgcaaaagc
     4261 tcgatagaag gtagacactg gttcacaatt ttcatattca gaacatgggc agatcttcga
     4321 atgcatcgcg cgcaccgtac gtctcgagga attcctgcag gatatctgga tccacgaagc
     4381 ttcccatggt gacgtcaccg gttctagata cctaggtgag ctctggtacc ctctagtcaa
     4441 ggccttaagt gagtcgtatt acgtagcccg ggcctagttc tagacattct ctaatgaaaa
     4501 aatctttcag ttgaaattga aaatgagtta aagttggagt ttttattgaa aacagatttc
     4561 cgtgtgatta gtgtttttag cgagtgtgac aggacagcga aaaaatatag aaacaagggg
     4621 ggaactgaaa agcttaggaa tgcattgaac atgagaaggg gaaggggaag gaacaaacta
     4681 gacaggaatt attggaa
//
