LOCUS       sequence-337183-        4672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV backbone for transgene expression in cortical interneurons under
            the control of the Dlx enhancer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Pouchelon G, Vergara J, McMahon J, Gorissen BL, Lin JD, 
            Vormstein-Schneider D, Niehaus JL, Burbridge TJ, Wester JC, Sherer 
            M, Fernandez-Otero M, Allaway KC, Pelkey K, Chittajallu R, McBain 
            CJ, Fan M, Nasse JS, Wildenberg GA, Fishell G, Dimidschstein J
  TITLE     A versatile viral toolkit for functional discovery in the nervous 
            system.
  JOURNAL   Cell Rep Methods. 2022 May 26;2(6):100225. doi: 
            10.1016/j.crmeth.2022.100225. eCollection 2022 Jun 20.
  PUBMED    35784651
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-05-26"; volume: "2(6):100225. doi"; pages: " 
            10.1016/j.crmeth.2022.100225. eCollection 2022 Jun 20"
COMMENT     Sequence Label: pAAV-VTKD1
FEATURES             Location/Qualifiers
     source          1..4672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   8..137
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          880..1012
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     995..1014
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    1055..1111
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1120..1708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1173..1193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1591..1602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1716..1940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1942..2082
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1942..2071
                     /label=AAV2 ITR
     rep_origin      2157..2612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2244..2263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2454..2475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2629..2648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2748..2770
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2808..2826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2894..2998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2999..3067,3068..3859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3217..3236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4030..4618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4519..4538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
       61 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
      121 catcactagg ggttcctgct agactgcaga gggcccgcgg ccgcacgcgt ttattcagaa
      181 tgttatgcac tcacagtggt ttggcatgca tctggtgaat tttttttaac gaaaaattag
      241 tgttggtttc gatgtatggt agcattctcc ctaacgtaat ttgaataatt cagcaaagcc
      301 ccactaccag ctgtacttct gcagcctctt ccattctttt cagcattata attttggtta
      361 attttcaatt ttaggtccta cgtctctgca atttgtgtat gaataacaga ataatttccc
      421 tcttttgttt cgcctttcct gttcctgaat ctaaataaag atggcttttt agtattaaaa
      481 gtggaagaaa attacaggta attatctttg acggtaaaaa cgctgtaatc agcgggctac
      541 atgaaaaatt actctaatta tggctgcatt taagagaatg gaaaaaaacc ttcttgtgga
      601 taaaaacctt aaattgtccc caatgtctgc ttcaaattgg atggcactgc agctggaggc
      661 tttgttcaga attgatcctg gggagctacg aacccaaagt ttcacagtag gaagctagat
      721 atcgtttaaa cttcctgcag cccgggctgg gcataaaagt cagggcagag ccatctattg
      781 cttacatttg cttctagcct gcaagtcgag gagcgcagcc ttccagaagc agagcgcggc
      841 gccttaagct gcagaagttg gtcgtgaggc actgggcacg taagtatcaa ggttacaaga
      901 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
      961 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
     1021 tcccagttca attacagctc ttaagaaagc tagcgaattc gagctcggta cccggggatc
     1081 ctctagagtc gacctgcagg catgcaagct tggcgcgcca atcaacctct ggattacaaa
     1141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     1201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     1261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     1321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     1381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     1441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     1501 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     1561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     1621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     1681 cggatctccc tttgggccgc ctccccgcct ggatcctgtg ccttctagtt gccagccatc
     1741 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1801 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1861 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1921 ggatgcggtg ggctctatgg caggaacccc tagtgatgga gttggccact ccctctctgc
     1981 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     2041 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     2101 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     2161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2221 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2281 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2341 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     2401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2461 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     2521 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2581 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     2641 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2701 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2761 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2821 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2881 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2941 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3001 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3061 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3121 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3181 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3241 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3301 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3361 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3421 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3481 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3541 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3601 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3661 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3721 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3781 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3841 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ct
//
