LOCUS       sequence-337195-       12111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM
  TITLE     Regulation of beta-catenin signaling by the B56 subunit of protein 
            phosphatase 2A.
  JOURNAL   Science. 1999 Mar 26. 283(5410):2089-91.
  PUBMED    10092233
REFERENCE   2  (bases 1 to 12111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1999 Mar 26. 283(5410):2089-91."
COMMENT     Sequence Label: V249 pCEP-4HA B56delta
FEATURES             Location/Qualifiers
     source          1..12111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             638..664
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             680..769
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    2592..2726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2616..2635)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2670..2689
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2869..2891)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3451..5240
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5542..7467)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGXGXGGGAGXGGGAGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGAGXGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GGGGXGGXGXGGAGXGGXGAGGAGAGGAGGAGAGGXGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7902..7920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7988..8092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8093..8161,8162..8953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8311..8330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9124..9712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9613..9632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10143..10288
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10331..11368
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11366..11385)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11410..11458
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccacgcgtca tatggcttac ccatacgatg ttccagatta
      661 cgctagcggc cgcatctttt acccatacga tgttcctgac tatgcgggct atccctatga
      721 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcgg
      781 ccgcccctat aaactgaaaa aggagaagga gccccccaag gttgccaaat gcacagccaa
      841 gcctagcagc tcgggcaagg atggtggagg cgagaacact gaggaggccc agccgcagcc
      901 ccagccccag ccccagcccc aagcccagtc tcagccaccg tcatccaaca agcgtcccag
      961 caatagcacg ccgcccccca cgcagctcag caaaatcaag tactcagggg ggccccagat
     1021 tgtcaagaag gagcgacggc aaagctcctc ccgcttcaac ctcagcaaga atcgggagct
     1081 gcagaagctt cctgccctga aagattcgcc aacccaggag cgggaggagc tgtttatcca
     1141 gaagctacgc cagtgctgtg tcctctttga cttcgtgtca gacccactca gtgacctcaa
     1201 attcaaggag gtgaagcggg caggactcaa cgagatggtg gagtacatca cccatagcca
     1261 tgatgttgtc actgaggcca tttaccctga ggctgtcacc atgttttcag tgaacctctt
     1321 ccggacgctg ccaccttcat cgaatcccac aggggctgag tttgacccag aggaagatga
     1381 gcccaccctg gaagctgctt ggccacatct ccagctcgtg tatgagttct tcttacgttt
     1441 ccttgagtct cctgatttcc agccaaacat agccaagaag tacatcgacc agaagtttgt
     1501 acttgctctc ctagacctat ttgacagtga ggatcctcga gagcgggact tcctcaagac
     1561 cattttgcat cgcatctatg gcaagttttt ggggctccgg gcttatatcc gtaggcagat
     1621 caaccacatc ttctacaggt tcatctacga gacggagcat cacaacggga ttgctgagct
     1681 cctggagatc ctgggcagca tcatcaatgg ctttgccctg ccccttaaag aagagcacaa
     1741 gatgttcctc atccgtgtcc tacttcccct tcacaaggtc aagtccctga gtgtctacca
     1801 ccctcagctg gcatactgtg tggtacaatt cctggagaag gagagcagtc tgactgagcc
     1861 ggtaattgtg ggacttctca agttttggcc caagacccac agccccaagg aggtgatgtt
     1921 cttgaatgag ctggaggaga ttctggacgt cattgaacct tctgagttca gcaaagtgat
     1981 ggaacccctc ttccgccagc tggccaagtg tgtctctagc ccccatttcc aggtggcaga
     2041 gcgtgctctc tattactgga acaatgagta catcatgagc ctgataagtg acaatgctgc
     2101 ccgagtcctc cccatcatgt tccctgcact ctacaggaac tccaagagcc actggaacaa
     2161 gacaatccat ggactgatct ataatgccct gaagttgttt atggaaatga atcagaagct
     2221 gtttgatgac tgcacacaac aatacaaggc agagaagcag aagggccggt tccgaatgaa
     2281 ggaaagggaa gagatgtggc aaaaaatcga ggagctggcc cggcttaatc cccagtatcc
     2341 catgttccga gcccctccac cactgccccc tgtgtactcg atggagacag agacccccac
     2401 agctgaggac atccagcttc tgaagaggac tgtggagact gaggctgttc agatgctaaa
     2461 agacatcaag aaggagaaag tgctgctgcg gaggaagtcg gagctgcccc aggacgtgta
     2521 caccatcaag gcactggagg cgcacaagcg ggcggaagag ttcctaactg ccagccagga
     2581 ggctctctga agatccagac atgataagat acattgatga gtttggacaa accacaacta
     2641 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2701 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2761 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2821 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2881 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     2941 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga
     3001 ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc
     3061 ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc
     3121 acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt
     3181 ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca
     3241 gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac
     3301 caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac
     3361 gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat
     3421 agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag
     3481 tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg
     3541 ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag
     3601 ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc
     3661 acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc
     3721 ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt
     3781 atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg
     3841 ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat
     3901 aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg
     3961 gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca
     4021 taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac
     4081 aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag
     4141 cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat
     4201 ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac
     4261 gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt
     4321 ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg
     4381 gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg
     4441 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc
     4501 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac
     4561 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc
     4621 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4681 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4741 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     4801 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc
     4861 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa
     4921 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     4981 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     5041 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     5101 tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt
     5161 agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt
     5221 cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg
     5281 ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg
     5341 agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat
     5401 gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg
     5461 atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag
     5521 ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc
     5581 ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa
     5641 ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt
     5701 aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat
     5761 atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg
     5821 tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg
     5881 tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata
     5941 tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc
     6001 caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc
     6061 acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg
     6121 tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg
     6181 cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc
     6241 tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga
     6301 tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct
     6361 ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact
     6421 acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc
     6481 ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     6541 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     6601 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc
     6661 tgcccctcct gcccctcctg ctcctgcccc tcctscccct cctgctcctg cccctcctgc
     6721 ccctcctgct cctgcccctc ctgctcctsc ccctcctsct cctgcccctc ctsctcctsc
     6781 ycctcctscc cctcctcccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc
     6841 ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     6901 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctsctcc
     6961 tgcccctcct scccctccts cccctcctcc tsctcctscc cctcctsccc ctcctcctsc
     7021 tcctscccct cctcctsctc ctscccctcc tscccctcct gcccctcctc ctsctcctgc
     7081 ccctcctcct sctcctsccc ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
     7141 tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc
     7201 tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac
     7261 accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc
     7321 tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc
     7381 actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg
     7441 tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt
     7501 attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc
     7561 caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct
     7621 ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat
     7681 cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc
     7741 tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca
     7801 gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg
     7861 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
     7921 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     7981 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8041 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8101 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8161 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8221 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8281 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     8341 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8401 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8461 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8521 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8581 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     8641 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8701 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8761 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8821 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8881 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8941 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9001 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9241 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     9301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc
     9781 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
     9841 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
     9901 gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     9961 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
    10021 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc
    10081 gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta
    10141 tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt
    10201 cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca
    10261 ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg
    10321 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
    10381 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
    10441 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
    10501 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
    10561 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
    10621 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
    10681 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
    10741 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
    10801 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
    10861 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
    10921 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
    10981 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
    11041 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
    11101 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
    11161 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
    11221 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
    11281 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
    11341 ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
    11401 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
    11461 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
    11521 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
    11581 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt
    11641 tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg
    11701 ttgcgtgggg tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc
    11761 tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca
    11821 cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc
    11881 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
    11941 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
    12001 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
    12061 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt t
//
