LOCUS       sequence-33720-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA
  TITLE     Single-molecule dynamics of gating in a neurotransmitter transporter
            homologue.
  JOURNAL   Nature. 2010 May 13. 465(7295):188-93.
  PUBMED    20463731
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 May 13. 465(7295):188-93."
COMMENT     Sequence Label: pQO18-TEV
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(172..276)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     344..362
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(386..400,401..406,407..423,424..430)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    406..422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    438..454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             514..543
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             553..573
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
       61 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
      301 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      361 gccctttcgt cttcacctcg agaaatcata aaaaatttat ttgctttgtg agcggataac
      421 aattataata gattcaattg tgagcggata acaatttcac acagaattcg aaggaactac
      481 aaaataaatt ataaggaggc actcaccatg tcgcatcacc atcaccatca ccatcaccat
      541 cacgatgacg atgaaaacct gtattttcag tccatggaag tgaaacgcga acattgggcg
      601 acccgcctgg gcctgattct ggcgatggcg ggcaacgcgg tgggcctggg caactttctg
      661 cgctttcctg tacaggcggc ggaaaacggc ggcggcgcgt ttatgattcc gtatattatt
      721 gcgtttctgc tggtgggcat tccgctgatg tggattgaat gggcgatggg ccgctatggc
      781 ggcgcgcagg gccacggcac cacacctgcg attttttatc tgctgtggcg caaccgcttt
      841 gcgaaaattc tgggcgtgtt tggcctgtgg attccgctgg tggtggcgat ttattatgtg
      901 tatattgaaa gctggaccct gggctttgcg atttaatttc
//
