LOCUS       sequence-33722-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA
  TITLE     Single-molecule dynamics of gating in a neurotransmitter transporter
            homologue.
  JOURNAL   Nature. 2010 May 13. 465(7295):188-93.
  PUBMED    20463731
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 May 13. 465(7295):188-93."
COMMENT     Sequence Label: pQO18 TEV-L400C
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(156..260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     328..346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(370..384,385..390,391..407,408..414)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    422..438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             498..527
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             537..557
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
       61 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
      301 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
      361 ctcgagaaat cataaaaaat ttatttgctt tgtgancgga taacaattat aatagattca
      421 attgtgagcg gataacaatt tcacacagaa ttcgaaggaa ctacaaaata aattataagg
      481 aggcactcac catgtcgcat caccatcacc atcaccatca ccatcacgat gacgatgaaa
      541 acctgtattt tcagtccatg gaagtgaaac gcgaacattg ggcgacccgc ctgggcctga
      601 ttctggcgat ggcgggcaac gcggtgggcc tgggcaactt tctgcgcttt cctgtacagg
      661 cggcggaaaa cggcggcggc gcgtttatga ttccgtatat tattgcgttt ctgctggtgg
      721 gcattccgct gatgtggatt gaatgggcga tgggccgcta tggcggcgcg ca
//
