LOCUS       sequence-33724-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA
  TITLE     Single-molecule dynamics of gating in a neurotransmitter transporter
            homologue.
  JOURNAL   Nature. 2010 May 13. 465(7295):188-93.
  PUBMED    20463731
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 May 13. 465(7295):188-93."
COMMENT     Sequence Label: pETO18G-AP
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..69)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(79..102)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(106..123)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gctttgttag cagccggatc ctcactcatg ccattcaatt ttctgagctt caaagatatc
       61 attaagcccc tcgagtccat gatggtgatg atggtgatga tgaccgctgc cgcgcggcac
      121 cagggtgccc gcgctttcat ggttgcggcg gcgttccgcc agaaacacca gaaaggtcag
      181 aaacagaaac aggccaataa tataaaagcg ggtaatccac acggtccaat gggtttcttc
      241 cataattttc ggaatatatt cccgggccca caccaccagc agcaccgcca gaaacgccgg
      301 ggtaatatag cgcatcacat aataataaat gcgcggcact ttaataatgc cgccgcggtt
      361 aatctcctcc cacgctttat ccgcgccaaa aatccaaaaa aaaataatca gttcggtcag
      421 gccaaaaaac accacgccaa tggtacccgc ccaaaaatcc atttcatcca ggcttttgtt
      481 cagaaacatc accagatgcg cgctaaaaaa cacaatcgcc gcggtccaca gcaccgcatg
      541 tttgcggctc agcttaagtt catcttccag aaacgcaatc atcggctgca taatcgcaat
      601 gctgctggtc aggcccgcaa aaaacagcag aaaaaaccac agaaagccca gaaaggtgcc
      661 gcccgcggtc tggctaaaaa tcgccggcag ggtaataaag cctaggttaa acgcgcccgc
      721 tttcgcaatc gccaccgcgt tcgccacgcc aaaaaacgcc accgccgccg gaatgctaat
      781 gctgccgccc agaatcactt ccgctttttc gttcanggtc gccgcggtca ggccgctcag
      841 cacaatatcc tgatctttac gtacatagct cgcataggta ataatcgcgc caaagcccag
      901 gctcagggta aaaaaaatct ggcccaccgc cgcaatccac acgccccga
//
