LOCUS       sequence-337244-       10353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC
            at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 
            170125.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MS2-adRNA (3'GAC)
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        88..417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      268..403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     330..349
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        465..512
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             531..905
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1035..1156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1072..1091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1126..1145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1205..1221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1205..1221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1218..1240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1229..1245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1253..1259,1260..1277,1278..1283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1436..1453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1607..2195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1687..1706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2366..3157,3158..3226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2989..3008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3227..3331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3406..3425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3597..3976
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3978..4176
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4131..4151
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4194..4374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4421..4546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5039..5272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5457..5501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5799..5916
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5967..6215
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5967..5987
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6138..6157
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6218..6236
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6259..6277
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6312..6625
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     6544..6564
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        6630..6648
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6671..6689
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6753..6964
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6983..6992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6989..7696
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7134..7152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7402..7421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7706..7762
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7763..8788
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    8804..9392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8857..8877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9275..9286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9464..9697
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9723..9740)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9729..9953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9999..10353,1..74)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10086..10105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10296..10317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
       61 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      241 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
      361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      421 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
      481 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
      541 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
      601 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
      661 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
      721 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
      781 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
      841 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
      901 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
      961 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     1021 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1141 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     1201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1261 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1381 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2041 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3361 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     3421 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     3481 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     3541 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     3601 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     3661 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     3721 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     3781 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     3841 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     3901 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     3961 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     4021 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     4081 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     4141 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     4201 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4261 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4321 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     4381 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     4441 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     4501 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     4561 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     4621 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     4681 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     4741 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     4801 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     4861 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     4921 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     4981 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     5041 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     5101 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     5161 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     5221 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     5281 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     5341 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     5401 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     5461 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     5521 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     5581 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     5641 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     5701 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     5761 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     5821 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5881 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     5941 gatccagttt ggttaattaa ggtaccgagg gcctatttcc catgattcct tcatatttgc
     6001 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     6061 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     6121 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     6181 tggctttata tatcttgtgg aaaggacgaa acaccgaaca tgaggatcac ccatgtcgca
     6241 cagcctgctg gctcttcaac atgaggatca cccatgtctt tttttcgtac tgagtcgccc
     6301 agtctcagat agatccgacg ccgccatctc taggcccgcg ccggccccct cgcacagact
     6361 tgtgggagaa gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta
     6421 actatagagg cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac
     6481 attttacatg ataggcttgg atttctataa gagatacaaa tactaaatta ttattttaaa
     6541 aaacagcaca aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa
     6601 atatcccttg gagaaaagcc ttgtttggaa catgaggatc acccatgtcg cacagcctgc
     6661 tggctcttca acatgaggat cacccatgtc tttttttgaa ttcgctagct aggtcttgaa
     6721 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     6781 cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt ggcgcggggt
     6841 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     6901 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggcttg ccgccagaac
     6961 acaggaccgg ttctagagcg ctgccaccat ggtgagcaag ggcgaggagg ataacatggc
     7021 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     7081 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     7141 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     7201 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     7261 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     7321 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     7381 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     7441 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     7501 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     7561 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     7621 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     7681 ggacgagctg tacaagggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga
     7741 tgtcgaagag aatcctggac cgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     7801 gtttctgatc gaaaagttcg acagcgtgtc cgacctgatg cagctctcgg agggcgaaga
     7861 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     7921 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     7981 gattccggaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt gcatctcccg
     8041 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     8101 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     8161 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     8221 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     8281 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     8341 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     8401 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     8461 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     8521 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     8581 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     8641 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     8701 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     8761 cagcactcgt ccgagggcaa aggaataaac gcgttaagtc gacaatcaac ctctggatta
     8821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8881 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     8941 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     9001 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     9061 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     9121 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     9181 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     9241 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     9301 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     9361 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     9421 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9481 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     9541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9661 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     9721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     9781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9841 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9901 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     9961 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
    10021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    10081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    10141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    10201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10321 aacactcaac cctatctcgg tctattcttt tga
//
