LOCUS       sequence-337253-       11412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Vp64-dCas9 activation system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Penev A, Bazley A, Shen M, Boeke JD, Savage SA, Sfeir A
  TITLE     Alternative splicing is a developmental switch for hTERT expression.
  JOURNAL   Mol Cell. 2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 
            10.1016/j.molcel.2021.03.033.
  PUBMED    33852895
REFERENCE   2  (bases 1 to 11412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 
            10.1016/j.molcel.2021.03.033."
COMMENT     Sequence Label: pLenti MS2-p65-HSF1 sfGFP
FEATURES             Location/Qualifiers
     source          1..11412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(114..134)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        119..448
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      299..434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     361..380
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        496..543
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             562..936
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1066..1187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1103..1122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1157..1176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1236..1252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1236..1252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1249..1271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1260..1276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1284..1290,1291..1308,1309..1314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1329..1350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1467..1484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1638..2226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1718..1737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2397..3188,3189..3257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3020..3039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3258..3362)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3437..3456)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3628..4007
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4009..4207
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4162..4182
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4225..4405
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4452..4577
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5070..5303
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5488..5532
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5830..5947
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6077..7255
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6308..7246
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7203..7223
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7262..7271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7271..7657
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             7712..7732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7748..8290
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8315..8686
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             8699..8752
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8756..9469
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             8756..9466
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(8783..8811)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9406..9426
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    9494..10082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9547..9567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9965..9976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10085..10101)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10086..10102)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10607..10787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10813..10830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10819..11043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11089..11412,1..105)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11176..11195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11386..11407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
       61 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
      121 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      181 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      241 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      301 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      361 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
      421 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
      481 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
      541 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
      601 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
      661 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
      721 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
      781 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
      841 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
      901 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
      961 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1021 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     1081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     1201 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1261 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1321 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1381 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1441 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2041 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2701 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3001 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3361 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     3481 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     3541 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     3601 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     3661 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3721 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3781 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3841 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3901 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3961 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     4021 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4081 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4141 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4201 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     4261 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4321 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4381 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4441 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4501 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4561 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4621 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4681 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4741 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4801 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4861 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4921 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4981 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     5041 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5101 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5161 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5221 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5281 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5341 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5401 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5461 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5521 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5581 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5641 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5701 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5761 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     5821 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     5881 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     5941 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta gctagctgca
     6001 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     6061 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     6121 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     6181 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     6241 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     6301 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     6361 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     6421 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     6481 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     6541 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     6601 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     6661 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     6721 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     6781 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     6841 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     6901 gagctcaaaa tgaaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     6961 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     7021 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     7081 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     7141 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     7201 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac
     7261 ggccaccatg gcttcaaact ttactcagtt cgtgctcgtg gacaatggtg ggacagggga
     7321 tgtgacagtg gctccttcta atttcgctaa tggggtggca gagtggatca gctccaactc
     7381 acggagccag gcctacaagg tgacatgcag cgtcaggcag tctagtgccc agaagagaaa
     7441 gtataccatc aaggtggagg tccccaaagt ggctacccag acagtgggcg gagtcgaact
     7501 gcctgtcgcc gcttggaggt cctacctgaa catggagctc actatcccaa ttttcgctac
     7561 caattctgac tgtgaactca tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc
     7621 tatcccttcc gccatcgccg ctaactcagg tatctacagc gctggaggag gtggaagcgg
     7681 aggaggagga agcggaggag gaggtagcgg acctaagaaa aagaggaagg tggcggccgc
     7741 tggatcccct tcagggcaga tcagcaacca ggccctggct ctggccccta gctccgctcc
     7801 agtgctggcc cagactatgg tgccctctag tgctatggtg cctctggccc agccacctgc
     7861 tccagcccct gtgctgaccc caggaccacc ccagtcactg agcgctccag tgcccaagtc
     7921 tacacaggcc ggcgagggga ctctgagtga agctctgctg cacctgcagt tcgacgctga
     7981 tgaggacctg ggagctctgc tggggaacag caccgatccc ggagtgttca cagatctggc
     8041 ctccgtggac aactctgagt ttcagcagct gctgaatcag ggcgtgtcca tgtctcatag
     8101 tacagccgaa ccaatgctga tggagtaccc cgaagccatt acccggctgg tgaccggcag
     8161 ccagcggccc cccgaccccg ctccaactcc cctgggaacc agcggcctgc ctaatgggct
     8221 gtccggagat gaagacttct caagcatcgc tgatatggac tttagtgccc tgctgtcaca
     8281 gatttcctct agtgggcagg gaggaggtgg aagcggcttc agcgtggaca ccagtgccct
     8341 gctggacctg ttcagcccct cggtgaccgt gcccgacatg agcctgcctg accttgacag
     8401 cagcctggcc agtatccaag agctcctgtc tccccaggag ccccccaggc ctcccgaggc
     8461 agagaacagc agcccggatt cagggaagca gctggtgcac tacacagcgc agccgctgtt
     8521 cctgctggac cccggctccg tggacaccgg gagcaacgac ctgccggtgc tgtttgagct
     8581 gggagagggc tcctacttct ccgaagggga cggcttcgcc gaggacccca ccatctccct
     8641 gctgacaggc tcggagcctc ccaaagccaa ggaccccact gtctcctgta ccagtggaga
     8701 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc caaccagcaa
     8761 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     8821 tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac
     8881 ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac
     8941 tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt
     9001 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga
     9061 cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat
     9121 cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgagta
     9181 caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc
     9241 taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag accattatca
     9301 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac
     9361 acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt
     9421 tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaatgag aattcgatat
     9481 caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9541 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9601 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9661 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9721 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9781 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9841 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9901 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9961 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    10021 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    10081 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
    10141 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
    10201 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
    10261 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
    10321 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
    10381 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
    10441 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
    10501 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
    10561 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
    10621 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10681 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10741 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
    10801 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
    10861 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
    10921 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
    10981 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
    11041 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
    11101 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    11161 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    11221 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    11281 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    11341 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    11401 aaactggaac aa
//
