LOCUS       sequence-337256-       12323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector carrying the MCP-ADAR2 with a nuclear export 
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MCP-ADAR2-DD
FEATURES             Location/Qualifiers
     source          1..12323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(131..151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        136..465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      316..451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     378..397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        513..560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             579..953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1083..1204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1120..1139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1174..1193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1253..1269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1253..1269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1266..1288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1277..1293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1301..1307,1308..1325,1326..1331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1346..1367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1484..1501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1655..2243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1735..1754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2414..3205,3206..3274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3037..3056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3275..3379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3454..3473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3645..4024
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4026..4224
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4179..4199
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4242..4422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4469..4594
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5087..5320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5505..5549
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5847..5964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6015..6263
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6015..6035
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6186..6205
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8145..8220
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8282..8493
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      8512..8521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10117..10143
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             10153..10209
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10210..10806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     10703..10723
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10822..11410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10875..10895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11293..11304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11482..11715
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11741..11758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11747..11971
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(12017..12323,1..122)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12104..12123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(12314..12323,1..12)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
       61 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      121 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      181 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
      421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
      481 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
      541 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
      601 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
      661 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
      721 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
      781 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
      841 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
      901 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
      961 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1021 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1081 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1201 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     3421 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3481 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     3541 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     3601 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     3661 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3721 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3781 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3841 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3901 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3961 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4021 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4081 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4141 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4201 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     4261 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4321 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4381 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     4441 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     4501 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     4561 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     4621 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     4681 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     4741 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     4801 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     4861 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     4921 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     4981 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5041 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5101 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5161 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5221 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     5281 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     5341 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     5401 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     5461 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     5521 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     5581 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     5641 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     5701 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     5761 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     5821 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     5881 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     5941 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     6001 ttaattaagg taccgagggc ctatttccca tgattccttc atatttgcat atacgataca
     6061 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     6121 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     6181 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     6241 tcttgtggaa aggacgaaac accggagagg gttgtaaatg agcacacaaa atacacatgc
     6301 taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt taataacttt
     6361 agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa aatgagcaaa
     6421 ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat aatagctact
     6481 ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc atcttcaaat
     6541 gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga cttcattagt
     6601 ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt aacaaattct
     6661 aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt taataaacaa
     6721 ttttccataa tacatttaac aacatgtgat ccagctgctt tttttacagc tttcatgtct
     6781 tctaaaacta attcataatt tttgtctttt aatgcaccaa tatttaatac catatcaatt
     6841 tctgttgcac catctttaat tgcttcagaa acttcgaatg cttttgtagc tgttgtgcat
     6901 gcacctagag gaaaacctac aacatttgtt attcctacat ttgtgccttt taataattct
     6961 ttacaatagc ttgttcaata tgaattaaca caaactgttg caaaatcaaa ttcaattgct
     7021 tcatcacata attgtttaat ttcagctttc gtagcatctt gttttaataa tgtgtgatct
     7081 atatatttgt ttagtttcat tttttctcct atatattcat ttttaatttt aattctttaa
     7141 taatttcgtc tactttaact ttagcgtttt gaacagattc accaacacct ataaaataaa
     7201 tttttagttt aggttcagtt ccacttgggc gaacagcaaa tcatgactta tcttctaaat
     7261 aaaattttag taagtcttgt cctggcatat tatacattcc atcgatgtag tcttcaacat
     7321 taacaacttt aagtccagca atttgagtta agggtgttgc tctcaatgat ttcattaatg
     7381 gttcaatttt taatttcttt tcttctggtt taaaattcaa gtttaaagtg aaagtgtaat
     7441 atgcacccat ttctttaaat aaatcttcta aatagtctac taatgtttta ttttgttttt
     7501 tataaaatca agcagcctct gctattaata tagaagcttg tattccatct ttatctctag
     7561 ctgagtcatc aattacatat ccataacttt cttcataagc aaaaacaaaa tttaatccgt
     7621 tatcttcttc tttagcaatt tctctaccca ttcatttaaa tccagttaaa gtttttacaa
     7681 tattaactcc atatttttca tgagcgattc tatcacccaa atcacttgtt acaaaacttg
     7741 aatatagagc cggatttttt ggaatgctat ttaagcgttt tagatttgat aattttcaat
     7801 caattaaaat tggtcctgtt tgatttccat ctaatcttac aaaatgacca tcatgtttta
     7861 ttgccattcc aaatctgtca gcatctgggt cattcataat aataatatct gcatcatgtt
     7921 taataccata ttcaagcggt atttttcatg caggatcaaa ttctggattt ggatttacaa
     7981 catttttaaa tgtttcatct tcaaatgcat gctcttcaac ctcaataacg ttatatcctg
     8041 attcacgtaa tatttttggg gtaaatttag ttcctgttcc attaactgcg ctaaaaataa
     8101 tttttaaatc ttttttagct tcttgctctt ttttgtacct ctctgtttta gagctagaaa
     8161 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     8221 ttttttgaat tcgctagcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     8281 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     8341 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     8401 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     8461 ctttttcgca acgggtttgc cgccagaaca caggaccggt tctagagcgc tgccaccatg
     8521 gcgtccaatt tcactcagtt tgtgctggtt gacaacggcg ggaccgggga cgttacggta
     8581 gccccctcaa actttgccaa cggtatagcg gagtggataa gcagcaattc taggagtcaa
     8641 gcatacaaag ttacatgcag cgtgcgccaa tctagcgctc agaatcgcaa gtacaccatt
     8701 aaagtagagg tccccaaggg agcctggaga agctatctta acatggagtt gaccatacca
     8761 atcttcgcta ccaactctga ctgtgaactc attgtgaaag ccatgcaagg tctgctcaag
     8821 gatggtaacc caattccgtc cgctatcgct gccaactctg ggatttacgg gggcagtggg
     8881 agcggtgcag gatctggtag tccagctggg ggaggagcac cgggtagcgg tggggggtct
     8941 cagctccatc tgccgcaagt tctggctgat gccgtgagca gactcgtgct aggcaaattc
     9001 ggcgacctga ccgacaactt cagcagccct cacgccagaa gaaaagtcct ggccggcgtg
     9061 gtcatgacaa ccggcacaga tgtgaaggac gccaaagtga tcagcgtgtc caccggcacc
     9121 aagtgcatca acggcgagta catgagcgac agaggactgg ccctgaacga ttgccacgcc
     9181 gagatcatca gcagaagaag cctgctgcgg ttcctataca cccagctgga actgtacctg
     9241 aacaacaagg acgaccagaa gcggtccatc ttccagaagt ctgagagagg cggcttccgg
     9301 ctgaaagaaa atgtgcagtt tcacctatac atctccacaa gcccttgcgg cgacgctcgg
     9361 atctttagcc cccacgagcc catcctggaa gaacctgccg acagacaccc caacagaaag
     9421 gcccggggcc agctgagaac aaagatcgaa agcggcgaag gcacaatccc cgtgcggagc
     9481 aatgcctcta tccaaacatg ggacggcgtg ctgcaaggcg agagactgct gacaatgagc
     9541 tgcagcgaca agatcgcccg gtggaatgtt gtgggcatcc agggatctct gctgagcatc
     9601 ttcgtggaac ccatctactt ctcgagcatc atcctgggca gcctgtacca cggcgatcac
     9661 ctgagcagag ccatgtacca gcggatcagc aatatcgagg acctgcctcc tctgtatacc
     9721 ctgaacaagc cactgctgag cggcatctcc aatgccgaag ctagacagcc tggcaaggcc
     9781 cctaacttca gcgtgaactg gacagtgggg gatagcgcca tcgaagtgat caatgccacc
     9841 accggcaaag acgaactagg aagagccagc agactgtgca agcacgccct gtattgcaga
     9901 tggatgagag tgcatgggaa agtgccttct catctgctga gaagcaagat caccaagcct
     9961 aacgtgtacc acgagagcaa gctggccgct aaagagtacc aggccgccaa ggccagactg
    10021 ttcaccgcct ttattaaggc cggactcggc gcctgggtcg agaagcctac agagcaggat
    10081 cagttcagcc tgacacctgg gagcggatcc ggctctctgc caccattgga aaggcttacg
    10141 ctgggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat
    10201 cctggaccga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
    10261 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
    10321 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
    10381 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
    10441 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
    10501 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
    10561 aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt
    10621 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
    10681 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
    10741 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
    10801 gcctgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
    10861 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    10921 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    10981 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    11041 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    11101 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    11161 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    11221 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    11281 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    11341 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    11401 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
    11461 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
    11521 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    11581 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    11641 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    11701 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
    11761 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
    11821 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    11881 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    11941 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
    12001 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    12061 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    12121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    12181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    12241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    12301 acgttcttta atagtggact ctt
//
