LOCUS       sequence-337258-        8150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PICASSO: Library expression vector precursor for paired dCas9+sgRNA 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Barber KW, Shrock E, Elledge SJ
  TITLE     CRISPR-based peptide library display and programmable microarray 
            self-assembly for rapid quantitative protein binding assays.
  JOURNAL   Mol Cell. 2021 Aug 4. pii: S1097-2765(21)00596-7. doi: 
            10.1016/j.molcel.2021.07.027.
  PUBMED    34390675
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 4. pii: S1097-2765(21)00596-7. doi: 
            10.1016/j.molcel.2021.07.027."
COMMENT     Sequence Label: pdCas9+sgRNA expression vector precursor
FEATURES             Location/Qualifiers
     source          1..8150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2559..2578
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(2664..2669,2670..2687,2688..2694)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2702..2718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2759..3436
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3469..3540
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3556..3583
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3611..3628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_RNA        3752..3827
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      complement(3884..4472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3964..3983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4643..5434,5435..5503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5266..5285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5504..5608)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(5640..6266)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     6171..6190
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        6282..6337
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    6318..6336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             6355..6366
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(6373..6399,6400..6402,6403..8150,1..739,740..742,
                     743..2326)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
       61 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
      121 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
      181 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
      241 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
      301 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
      361 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
      421 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
      481 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
      541 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
      601 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
      661 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
      721 aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga
      781 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
      841 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
      901 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
      961 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     1021 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     1081 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     1141 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     1201 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     1261 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     1321 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     1381 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     1441 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     1501 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa
     1561 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     1621 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     1681 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc
     1741 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga
     1801 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     1861 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     1921 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     1981 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     2041 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     2101 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     2161 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     2221 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac
     2281 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgacggtg gaggaggttc
     2341 tcgatcgtgt acacgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
     2401 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     2461 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     2521 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     2581 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     2641 gagatcgttt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     2701 attgtgagcg gataacaatt tcagaattca aaagatcttt taagaaggag atatacatat
     2761 ggcgagtagc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
     2821 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
     2881 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
     2941 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
     3001 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
     3061 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
     3121 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
     3181 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
     3241 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
     3301 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
     3361 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
     3421 ctccaccggt gcttaaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga
     3481 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3541 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac ctagggatat
     3601 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     3661 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     3721 tgagagggcc gcggcaaagc cgttggtctc agttttagag ctagaaatag caagttaaaa
     3781 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgaagct
     3841 tgggcccagc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
     3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
     4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatggtt gtcagaagta
     5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     5641 taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
     5701 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
     5761 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
     5821 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
     5881 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
     5941 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
     6001 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
     6061 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
     6121 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
     6181 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
     6241 ttacttttat ctaatctaga catcattaat tcctaatttt tgttgacact ctatcgttga
     6301 tagagttatt ttaccactcc ctatcagtga tagagaaaag aattcaaaag atctaaagag
     6361 gagaaaggat ctatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
     6421 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
     6481 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
     6541 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
     6601 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     6661 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     6721 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     6781 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     6841 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     6901 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     6961 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     7021 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     7081 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     7141 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     7201 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     7261 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
     7321 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     7381 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     7441 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     7501 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
     7561 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     7621 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
     7681 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
     7741 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     7801 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     7861 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     7921 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa
     7981 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     8041 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     8101 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
//
