LOCUS       sequence-33726-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Zhao Y, Terry D, Shi L, Weinstein H, Blanchard SC, Javitch JA
  TITLE     Single-molecule dynamics of gating in a neurotransmitter transporter
            homologue.
  JOURNAL   Nature. 2010 May 13. 465(7295):188-93.
  PUBMED    20463731
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 May 13. 465(7295):188-93."
COMMENT     Sequence Label: pETO18G-H7C/R86C
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..56)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(60..77)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cgggctttgt tagcagccgg atcctcgagt caatgatggt gatgatggtg atgatgaccg
       61 ctgccgcgcg gcaccagggt gcccgcgctt tcatggttgc ggcggcgttc cgccagaaac
      121 accagaaagg tcagaaacag aaacaggcca ataatataaa agcgggtaat ccacacggtc
      181 caatgggttt cttccataat tttcggaata tattcccggg cccacaccac cagcagcacc
      241 gccagaaacg ccggggtaat atagcgcatc acataataat aaatgcgcgg cactttaata
      301 atgccgccgc ggttaatctc ctcccacgct ttatccgcgc caaaaatcca aaaaaaaata
      361 atcagttcgg tcaggccaaa aaacaccacg ccaatggtac ccgcccaaaa atccatttca
      421 tccaggcttt tgttcagaaa catcaccaga tgcgcgctaa aaaacacaat cgccgcggtc
      481 cacagcaccg catgtttgcg gctcagctta agttcatctt ccagaaacgc aatcatcggc
      541 tgcataatcg caatgctgct ggtcaggccc gcaaaaaaca gcagaaaaaa ccacagaaag
      601 cccagaaagg tgccgcccgc ggtctggcta aaaatcgccg gcagggtaat aaagcctagg
      661 ttaaacgcgc ccgctttcgc aatcgccacc gcgttcgcca cgccaaaaaa cgccaccgcc
      721 gccggaatgc taatgctgcc gcccagaatc acttccgctt tttcgttcan ggtcgccgcg
      781 gtcaggccgc tcagcacaat atcctgatct ttacgtacat agctcgcata ggtaataatc
      841 gcgccaaagc ccaggctcag ggtaaaaaaa atctggccca ccgccgcaat ccacacgccc
      901 ggatctttca gtttttcaaa atccggggtc cacagaaagt tc
//
