LOCUS       sequence-337281-        9240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M
  TITLE     Records of RNA locations in living yeast revealed through covalent 
            marks.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 
            10.1073/pnas.1921408117. Epub 2020 Sep 9.
  PUBMED    32907940
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1921408117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-09-22- 22"; volume: "117"; issue: "38"; pages: 
            "23539-23547"
COMMENT     Sequence Label: pHCMM4
FEATURES             Location/Qualifiers
     source          1..9240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    75..96
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(111..116,117..134,135..141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     154..176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     173..189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     173..189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     208..228
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        210..228
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2003..2713
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2148..2166)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2416..2435
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(2849..3943)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(3944..4349)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     terminator      4362..4609
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4378..4396)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4627..4646)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4628..4646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4656..4673)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4656..4672)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4665..4687)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4813..5268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4900..4919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5110..5131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5528..6190)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6191..6378)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6525..6544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6644..6666
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6704..6722)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6770..7273
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7303..7407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7408..7476,7477..8268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7626..7645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8439..9027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8928..8947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9181..9198
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
       61 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      121 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      181 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      241 gagctcattt ggcgagcgtt ggttggtgga tcaagcccac gcgtaggcaa tcctcgagca
      301 gatccgccag gcgtgtatat atagcgtgga tggccaggca actttagtgc tgacacatac
      361 aggcatatat atatgtgtgc gacgacacat gatcatatgg catgcatgtg ctctgtatgt
      421 atataaaact cttgttttct tcttttctct aaatattctt tccttataca ttaggacctt
      481 tgcagcataa attactatac ttctatagac acgcaaacac aaatacacac actaatctag
      541 aactagtggg atgtcctcca accgtgttct agacttgttt aagccatttg aatctttcct
      601 gccggaagtg attgctccag aaaggaaggt tccatacaac cagaaactta tctggacagg
      661 cgtttctcta ctgatctttt tgattctggg ccagattccg ctgtacggga tcgtgtccag
      721 tgagacttcc gaccctctgt actggctacg tgccatgctg gcctccaacc gtggtacttt
      781 actggaattg ggtgtttcgc ccatcatcac ttcatctatg attttccaat ttttgcaggg
      841 tactcagctt ttacaaatca gacctgagag caagcaggac agagagctgt tccaaattgc
      901 tcaaaaggtg tgcgctatta ttctgatctt gggccaagcc cttgtggtcg tcatgacagg
      961 taactacggt gccccttcgg acctcggatt gcccatctgt ttgttgttaa tctttcaatt
     1021 gatgtttgca tcgctgattg tgatgttatt agacgaattg ctatctaagg gttacggctt
     1081 gggttccggt atttctctgt tcacggcaac caatattgcc gaacaaattt tctggagagc
     1141 gtttgctcct actacagtca attccggtcg tggtaaggag ttcgaaggtg ctgtgattgc
     1201 ctttttccat cttttggctg tcagaaagga caagaaaaga gcccttgtcg aggcctttta
     1261 ccgtaccaat ctacctaata tgttccaagt gttgatgacc gtggccatct tcctctttgt
     1321 tttatattta caaggcttcc gttacgaatt gcccatcagg tcaaccaaag tgagaggtca
     1381 aattggtatc taccccatca aactctttta tacttccaac accccaatca tgttgcagag
     1441 tgcattgact tctaacattt tcttgatctc tcaaatcctt ttccagaaat acccaaccaa
     1501 tccattgatt cgtttgatcg gtgtttgggg tatcaggccg ggcacccagg gccctcaaat
     1561 ggccttgagc gggttggcct actacatcca accattaatg tctttatccg aagctcttct
     1621 ggaccctatc aagaccatcg tctacatcac atttgttctt ggttcatgcg cagtattttc
     1681 caagacatgg atcgaaatct ccggcacttc cccacgtgac attgccaaac aattcaaaga
     1741 tcaaggcatg gtcattaacg gtaagagaga aacctccatt tacagagaat tgaagaagat
     1801 cattccaact gctgctgctt tcggcggtgc taccatcggt gctctttctg ttggctccga
     1861 cctactaggt actttaggtt ctggggcatc cattttgatg gctactacca ccatctacgg
     1921 ctactacgaa gctgccgcca aggaaggtgg gtttactaag aacctcgttc caggattttc
     1981 tgatttgatg ggatcccccg ggatggtgag caagggcgag gaggataaca tggccatcat
     2041 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     2101 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     2161 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     2221 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     2281 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     2341 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     2401 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     2461 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     2521 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     2581 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     2641 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     2701 gctgtacaag taactgcagg aattcgatat caagcttatc gataccgcta ccctatgaac
     2761 atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt
     2821 acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga
     2881 cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg
     2941 catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg
     3001 acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg
     3061 gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg
     3121 gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt
     3181 catcggagat gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg
     3241 ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag
     3301 gaaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa
     3361 cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag
     3421 cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga
     3481 caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga
     3541 caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag
     3601 agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt
     3661 ttagtaaacc ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac
     3721 ctaacaaaac ggcatcagcc ttcttggagg cttccagcgc ctcatctgga agtggaacac
     3781 ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg
     3841 aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa
     3901 cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt
     3961 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
     4021 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat
     4081 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc
     4141 gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga
     4201 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct
     4261 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca
     4321 tcttacgata cctgagtatt cccacagttg tcgacctcga gtcatgtaat tagttatgtc
     4381 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     4441 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     4501 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
     4561 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc
     4621 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4681 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4741 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4801 aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     5281 ttttctcctt acgcatctgt gcggtatttc acaccgcata gatccgtcga gttcaagaga
     5341 aaaaaaaaga aaaagcaaaa agaaaaaagg aaagcgcgcc tcgttcagaa tgacacgtat
     5401 agaatgatgc attaccttgt catcttcagt atcatactgt tcgtatacat acttactgac
     5461 attcataggt atacatatat acacatgtat atatatcgta tgctgcagct ttaaataatc
     5521 ggtgtcacta cataagaaca cctttggtgg agggaacatc gttggtacca ttgggcgagg
     5581 tggcttctct tatggcaacc gcaagagcct tgaacgcact ctcactacgg tgatgatcat
     5641 tcttgcctcg cagacaatca acgtggaggg taattctgct agcctctgca aagctttcaa
     5701 gaaaatgcgg gatcatctcg caagagagat ctcctacttt ctccctttgc aaaccaagtt
     5761 cgacaactgc gtacggcctg ttcgaaagat ctaccaccgc tctggaaagt gcctcatcca
     5821 aaggcgcaaa tcctgatcca aaccttttta ctccacgcac ggcccctagg gcctctttaa
     5881 aagcttgacc gagagcaatc ccgcagtctt cagtggtgtg atggtcgtct atgtgtaagt
     5941 caccaatgca ctcaacgatt agcgaccagc cggaatgctt ggccagagca tgtatcatat
     6001 ggtccagaaa ccctatacct gtgtggacgt taatcacttg cgattgtgtg gcctgttctg
     6061 ctactgcttc tgcctctttt tctgggaaga tcgagtgctc tatcgctagg ggaccaccct
     6121 ttaaagagat cgcaatctga atcttggttt catttgtaat acgctttact agggctttct
     6181 gctctgtcat ctttgccttc gtttatcttg cctgctcatt ttttagtata ttcttcgaag
     6241 aaatcacatt actttatata atgtataatt cattatgtga taatgccaat cgctaagaaa
     6301 aaaaaagagt catccgctag gtggaaaaaa aaaaatgaaa atcattaccg aggcataaaa
     6361 aaatatagag tgtactagag gaggccaaga gtaatagaaa aagaaaattg cgggaaagga
     6421 ctgtgttatg acttccctga ctaatgccgt gttcaaacga tacctggcag tgactcctag
     6481 cgctcaccaa gctcttaaaa cgggaattta tggtgcactc tcagtacaat ctgctctgat
     6541 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6601 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     6661 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     6721 tttttatagg ttaatgtcat gataataatg gtttcttagg gtccttttca tcacgtgcta
     6781 taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa
     6841 aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg
     6901 atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat
     6961 tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact
     7021 tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt
     7081 aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt
     7141 aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata
     7201 aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta
     7261 caggcaagcg atccgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7621 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7921 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8101 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9181 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
//
