LOCUS       sequence-337289-        5587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ExbD T42C protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5587)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP1392
FEATURES             Location/Qualifiers
     source          1..5587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(508..525)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      678..764
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      856..883
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        902..993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(994..1062,1063..1854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1212..1231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1896..2351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1983..2002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2193..2214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2462..3050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2951..2970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3222..3239
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3405..3427
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3565..3584
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atggcaatgc atcttaacga aaacctcgac gataacggcg aaatgcatga tatcaacgtg
       61 acgccgttta tcgacgtgat gttggttctg ctgattatct ttatggtggc ggcaccgtta
      121 gcgtgcgtag atgtgaaggt gaacttgcct gcttctacca gcacgccgca gccgcgcccg
      181 gaaaaaccgg tttatctgtc ggtgaaggca gacaactcga tgtttatcgg taacgatccg
      241 gtcaccgatg aaacaatgat tacggcgttg aatgcgttaa ccgaaggcaa gaaagacacc
      301 accatcttct tccgagcgga caaaaccgtc gattacgaga cgttgatgaa ggtaatggat
      361 acgctgcatc aggcgggtta cctgaagata ggtctggtcg gcgaagaaac cgccaaagcg
      421 aagtaaccat ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggctg
      481 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
      541 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
      601 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
      661 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      721 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
      781 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
      841 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
      901 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1201 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     1261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1501 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1861 gaccaagttt actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag
     1921 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1981 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2041 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2101 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2161 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2221 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     2281 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2341 gtttacaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2581 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3061 acgcggcctt tttacggttc ctggcctttt tacggttcct ggccttttgc tggccttttg
     3121 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3181 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3241 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3301 gcatagggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3361 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3421 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag aaggagatgg cgcccaacag
     3481 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     3541 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     3601 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctgct catgtttgac
     3661 agcttatcat cgattcagct tttcagccgc cgccagaacg tcgtccggct gatgcctaaa
     3721 taattcgccg ctgctgtttt atcgccatta aatttctcca gtgcctgttg tggtgtcagt
     3781 aagcgtggag cgggagtttt cgccgactcg cgcgccagtt ccggcagtag cagctgcaaa
     3841 aattgcggcg ttaaatccgg cgtcggttcc acacttaaaa atagcgccag tcgctccatc
     3901 atattgcgca gttcacgaat attgcccggc cagtcgtagt gcaccagcac ggtttcgctt
     3961 gcctgtaatc cctggcgtaa tgcggcagaa aacggggtgg agagcgccgc cagagacact
     4021 ttcaaaaagc tttccgccag tggcagaata tccgccaccc gctcgcgcag tggtggcaat
     4081 tgcagacgca aaatactcag ccgataaaac aggtcacggc gaaactgccc ttgccgcata
     4141 tcttcttcca gattgcagtg agtggcgcta atgacccgca catccaccgg aacaggctga
     4201 tgcccgccga cgcgggtgac ctctttttct tccagcaccc gcagcagccg ggtctgcaac
     4261 ggcagcggca tttcgccaat ctcatcgaga aacagcgtac ctccgtgggc gatttcaaac
     4321 agcccggcgc gaccgccgcg tcgcgagccg gtaaacgccc cttcctcata gccaaacagt
     4381 tctgcttcca gcagcgattc ggcaatcgcc ccgcagttga ctgcaacaaa cggatgcgac
     4441 tttttgccct gtcgcgcatc gtggcgggca aaatattccc gatgaatcgc ctgggccgcc
     4501 agctctttgc ccgtccccgt ttccccctca atcaacaccg ctgcactgga gcgggcatac
     4561 agcaaaatag tctgccgtac ttgttccatc tgtggtgatt gaccgagcat atcgcccagc
     4621 acgtaacgag ttctcagggc gttgcgggtg gcatcgtgag tgttatggcg taacgacatg
     4681 cgcgtcatat ccagcgcatc gctgaacgcc tggcgcacgg tggcggcgga atagataaaa
     4741 attccggtca ttccggcttc ttctgccaga tcggtaatca gccccgcgcc gaccaccgct
     4801 tcggtgccgt tagcttttag ctcgttaatc tgcccgcgtg cgtcttcttc ggtaatgtag
     4861 ctacgttgat cgaggcgcaa attaaaggtt ttttgaaacg ccaccagtgc cggaatagtt
     4921 tcctgataag tgacaacgcc gatagaagag gtgagttttc cggcttttgc cagtgcctgt
     4981 aacacatcgt agccgctcgg tttaatcaaa ataactggca ctgacaggcg gcttttcagg
     5041 tacgcgccgt tagatccagc ggcgatgatg gcgtcacagc gttcgtttgc cagtttcttg
     5101 tggatgtagg tcaccgcttt ttcaaagcca agctggatag gggtaatgtt cgccaggtga
     5161 tcaaactcga ggctgatatc gcgaaacagc tcgaacaggc gcgttacaga taccgtccag
     5221 ataaccggtt tgtcgtcatt aagccgtggt ggatgtgcca tagcgcaccg caaagttaag
     5281 aaaccgaata ttgggtttag tcttgtttca taattgttgc aatgaaacgc ggtgaaacat
     5341 tgcctgaaac gttaactgaa acgcatattt gcggattagt tcatgacttt atctctaaca
     5401 aattgaaatt aaacatttaa ttttattaag gcaattgtgg cacacccctt gctttgtctt
     5461 tatcaacgca aataacaagt tgataacaag ctagcaggag gaattcacca tggacctgga
     5521 tctggaagcc agcgccgctg cgcatccggt tcgtgtcgca caaaaattac gcgcaggata
     5581 atatccg
//
