LOCUS       sequence-33729-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Klotzsche M, Ehrt S, Schnappinger D
  TITLE     Improved tetracycline repressors for gene silencing in mycobacteria.
  JOURNAL   Nucleic Acids Res. 2009 Apr;37(6):1778-88. doi: 10.1093/nar/gkp015. 
            Epub 2009 Jan 27.
  PUBMED    19174563
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkp015"; journalName: "Nucleic Acids Res"; date: 
            "2009-04"; volume: "37"; issue: "6"; pages: "1778-88"
COMMENT     Sequence Label: pGMCtN-P38-sspBmyc
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    579..603
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggacctcta gggtcccctg cagcttaagc aactatgtat aaaaaagttg ctattacttc
       61 acgacgcgca gggccgggcg accgccgcgc ggcggctggg gcggctcatc gtccgggtgg
      121 gtgtcatcgt cgtggtccgg cttgtcgccg tcgatcaccg acatcacggt ctcgttgtcg
      181 gccgaggcct cctcgtcgtt catgatcgag gtgtcctcgt cgtaggccgc ctccggctcg
      241 aacatggtgc cggcgccgtt ctcgcgggcg tagatggcca gcacggccgc cagcggcacc
      301 gagacctgcc gcgggatgcc accgaaacgc gcgttgaagc gcacctcgtc gttcgccagc
      361 tccaggttgc cgacggcgcg cggcgcgatg ttcagcacga tctggccgtc gcgcgcgtac
      421 tccatcggca cctgcacgcc cggcagggtc acgtccacca ccaggtgcgg ggtcagctgg
      481 ttgtccagca accactcgta gaaggcgcgc agcaggtagg gccggcgcgg ggtcagctgc
      541 gacaggtcca tatgtatatc tccttcgcat gcctcgagcc cactttgtac aagaaagctg
      601 ggtcggatca acggcctggt cgacaagggc aatacggtga tcgtcatcga acataacctg
      661 gacgtgatcg gccgtcaact cgttgaggta ccggtcggat tcaaatggtt cgtcgacggt
      721 ctgatcccgc ggtgtgcacc gatgccctca cggttccagc ggtcgaccag gtcctcgccg
      781 tgacgcccct tgatcgcaaa cgccaccgaa tgcaccacgg ccacagccaa agccgacacg
      841 ctggttacca cgacgatgtt cacggggggc cctcgcggat ctggcccaac tgctggactt
      901 ccaccgaagc aatcgagccg atgtcacgct gca
//
