LOCUS       sequence-33730-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Klotzsche M, Ehrt S, Schnappinger D
  TITLE     Improved tetracycline repressors for gene silencing in mycobacteria.
  JOURNAL   Nucleic Acids Res. 2009 Apr;37(6):1778-88. doi: 10.1093/nar/gkp015. 
            Epub 2009 Jan 27.
  PUBMED    19174563
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkp015"; journalName: "Nucleic Acids Res"; date: 
            "2009-04"; volume: "37"; issue: "6"; pages: "1778-88"
COMMENT     Sequence Label: pGMCtN-P38-sspBmyc
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(517..541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaagtttgt acaaaaaagc aggcttattg gatcacgccg cgagcgtgtc ggtgttcgaa
       61 gacctgggaa tccagcaggc gcccaacagc accgtctgct ccgtcttctc cagaaaggcg
      121 actacgcggt cctcctcgtc ggtgggtacg cagccgaatg cccgcgggtc gcccacccgc
      181 accagttgca gcgtgacatc ggctcgattg cttcggtgga agtccagcag ttgggccaga
      241 tccgcgaggg ccccccgtga acatcgtcgt ggtaaccagc gtgtcggctt tggctgtggc
      301 cgtggtgcat tcggtggcgt ttgcgatcaa ggggcgtcac ggcgaggacc tggtcgaccg
      361 ctggaaccgt gagggcatcg gtgcacaccg cgggatcaga ccgtcgacga accatttgaa
      421 tccgaccggt acctcaacga gttgacggcc gatcacgtcc aggttatgtt cgatgacgat
      481 caccgtattg cccttgtcga ccaggccgtt gatccgaccc agctttcttg tacaaagtgg
      541 gctcgaggca tgcgaaggag atatacatat ggacctgtcg cagctgaccc cgcgccggcc
      601 ctacctgctg cgcgccttct acgagtggtt gctggacaac cagctgaccc cgcacctggt
      661 ggtggacgtg accctgccgg gcgtgcaggt gccgatggag tacgcgcgcg acggccagat
      721 cgtgctgaac atcgcgccgc gcgccgtcgg caacctggag ctggcgaacg acgaggtgcg
      781 cttcaacgcg cgtttcggtg gcatcccgcg gcaggtctcg gtgccgctgg cggccgtgct
      841 ggccatctac gcccgcgaga acggcgccgg caccatgttc gagccgga
//
