LOCUS       sequence-337302-       11141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of
            yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M
  TITLE     Records of RNA locations in living yeast revealed through covalent 
            marks.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 
            10.1073/pnas.1921408117. Epub 2020 Sep 9.
  PUBMED    32907940
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1921408117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-09-22- 22"; volume: "117"; issue: "38"; pages: 
            "23539-23547"
COMMENT     Sequence Label: pHCMM1
FEATURES             Location/Qualifiers
     source          1..11141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        391..804
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             825..1538
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             825..1538
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(4373..5176)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      5498..5684
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5553..5577)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5692..5709)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5692..5708)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5701..5723)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6145..7491
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(7629..8723)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8724..9129)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(9149..9167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9235..9339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9340..9408,9409..10200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9558..9577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10371..10959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10860..10879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11113..11130
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttac
      241 aatgcatact ttgtacgttc aaaatacaat gcagtagata tatttatgca tattacatat
      301 aatacatatc acataggaag caacaggcgc gttggacttt taattttcga ggaccgcgaa
      361 tccttacatc acacccaatc ccccacaagt gatcccccac acaccatagc ttcaaaatgt
      421 ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc
      481 aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat
      541 tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg
      601 aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaaatttttt tttttgattt
      661 ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt caatttctca
      721 agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc tcattagaaa
      781 gaaagcatag caatctaatc taagttttaa ttacaaaaga gctcatgtct aaaggtgaag
      841 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
      901 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
      961 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttaactt
     1021 atggtgttca atgtttttct agatacccag atcatatgaa acaacatgac tttttcaagt
     1081 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
     1141 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
     1201 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata
     1261 actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca
     1321 aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata
     1381 ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc actcaatctg
     1441 ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa tttgttactg
     1501 ctgctggtat tacccatggt atggatgaat tgtacaaaag actagtaatg gctgatatta
     1561 ctgataagac agctgaacaa ttggaaaact tgaatattca agatgaccaa aagcaagccg
     1621 ccactggttc agaaagccaa tctgttgaaa actcttctgc atcattatat gttggtgact
     1681 tagaaccttc tgtttccgaa gcccacttat atgatatctt ctctccaatc ggttcagtct
     1741 cctccattcg tgtctgtcgt gatgccatca ctaagacctc tttgggctat gcttatgtta
     1801 actttaacga ccatgaagcc ggcagaaaag caattgagca attgaactac actccaatca
     1861 agggtagatt atgccgtatt atgtggtctc aacgtgaccc atcattgaga aagaagggtt
     1921 ctggtaacat ctttatcaag aacttgcacc ctgatattga caacaaggct ttgtatgaca
     1981 ctttctctgt gtttggtgac atcttgtcca gcaagattgc caccgacgaa aacggaaaat
     2041 ccaagggttt tgggtttgtt cacttcgaag aagaaggtgc tgccaaggaa gctattgatg
     2101 ctttgaatgg tatgctgttg aacggtcaag aaatttatgt tgctcctcac ttgtccagaa
     2161 aggaacgtga ctctcaattg gaagagacta aggcacatta cactaacctt tatgtgaaaa
     2221 acatcaactc cgaaactact gacgaacaat tccaagaatt gtttgccaaa tttggtccaa
     2281 ttgtttctgc ctctttggaa aaggatgctg atggaaaatt gaagggtttc gggtttgtta
     2341 actacgaaaa gcatgaagac gctgtgaaag ctgttgaagc tttgaatgac tctgaactaa
     2401 atggagaaaa gttatacgtt ggtcgtgccc aaaagaagaa tgaacgtatg catgtcttga
     2461 agaagcaata cgaagcttac agattggaaa aaatggccaa gtaccaaggt gttaatttgt
     2521 ttgttaagaa cttagatgac agcgttgatg acgaaaagtt ggaagaagaa tttgctccat
     2581 atggtactat cacttctgca aaggttatga gaaccgaaaa cggtaagtct aagggttttg
     2641 gttttgtttg tttctcaact ccagaggaag ctactaaggc cattacagaa aagaaccaac
     2701 aaattgttgc tggtaagcca ttatacgttg ccattgctca aagactagtc atgacagtta
     2761 ttcagaagtc gtctcctaca gtgaaaggag tgaagacaac aacatcagat tgcaccgaaa
     2821 gacgtcactc atcgagttca ccttgcaaaa tgagtcaaaa cggaagcagt ggatactatg
     2881 aaggagatgt tttactggat gatgatgctc ttgtttcgat gccagaagca acgctcattg
     2941 agtgctttgg taatctggct gtcacaaaaa gacctgcaca tctgagacct ccggcagctt
     3001 tctgtctacc gtttgtatca atgaacaacg atttgacgag cggacatgcc ttgaaacata
     3061 tcacattgac atcagtattc gacgcactac atccgtacgt tgccatgccg tacatcaaca
     3121 gaatggatat atgctctgca atgaacgtat attggatcag aaactgtttg gaagctgaac
     3181 aatccgattt atttcatcga ttcgctctgg aaatgcaagt acatttgagt gcatgttttg
     3241 gatgtcgcgt tgtcctggat atctatggat caaccagaaa cggattcggt acccgattct
     3301 gtgatgttga tatgtcactt tctttttcac cgagtccacc ttcatgggca accaattcgg
     3361 atcgagtgat gagagctgtt gcgaaggcac ttgtcgattt tccgaaagca gttgatgaga
     3421 gatatgtcaa cgccaaagtt ccaattgtca gattcagaag cagtgacatg gatatggaag
     3481 cagacatcag ttacaagaat gatttggctc ttcacaatac tcagcttttg caacaatact
     3541 gcaaatggga tcccgaaaga ctaccgactc ttggagtttg ggtcaaagcg tgggcgaaac
     3601 gaagtggagt tggagatgca tcaaaaggat cgctgtcttc atatgcttgg attgtgatgc
     3661 ttattcatta tcttcagcaa gtcgaaccaa ttcctgtgtt gccatgtctt caggaaatga
     3721 accatcagaa aagtgaaaat gtctacgtcc aaggttacaa cacgtattat tggaaattcg
     3781 tagacactgc tcgtacacga cgttgtcgtg cttctgtcgt tgatttgttc gttggattct
     3841 tggattacta tgccacatac tttgactact ccacaaatgt tatccagatg gtgtccaaga
     3901 aattggaatt caaaccggat cgctggtgca agtatccaat gtgtattgct gatccgttcg
     3961 agacggatca taatttggca caaggtgttg atatgccaat gtttgaatac attagatcgt
     4021 gtatggaaca ctcgaagaaa gtattcacgg atcgccgcat gcgctcggag tttctttcgg
     4081 gatacgggtt cgacgttgac gaatttgatg caaggcatcg aggcgaaatg aacatggaaa
     4141 tggcgtcaca atttggggaa ttccttcttc acaagtgcat aatggtgaaa caagccccga
     4201 atcgtcaatt ccgtgatcgc agtatgagtc aatcgacgag cataagcaac acatcgagta
     4261 tttcctcgtc gggactgcat taattaaccg catagggtaa taactgatat aattaaattg
     4321 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
     4381 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
     4441 ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
     4501 tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
     4561 aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
     4621 tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
     4681 tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
     4741 ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
     4801 ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
     4861 agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     4921 aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     4981 tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     5041 ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     5101 gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     5161 atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg
     5221 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     5281 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     5341 ttcaaagaaa ccggggtacc taactttgga cttcttcgcc agaggtttgg tcaagtctcc
     5401 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca
     5461 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat
     5521 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     5581 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     5641 tcaggtatag catgaggtcg ctcttattga ccacacctct accgctcgag cactggccgt
     5701 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5761 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5821 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     5881 gtgcggtatt tcacaccgca tttatcggat cgtacttgtt acccatcatt gaattttgaa
     5941 catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat
     6001 atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca
     6061 tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt
     6121 gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     6181 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     6241 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     6301 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     6361 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     6421 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     6481 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     6541 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     6601 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     6661 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     6721 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     6781 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     6841 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     6901 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     6961 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     7021 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     7081 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagcta gagaatagga
     7141 acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg
     7201 agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat
     7261 atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg
     7321 tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc
     7381 ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc
     7441 aattggatta gtctcatcct tcaatgctat catttccttt gatattggat cgatccgatg
     7501 ataagctgtc aaacatgaga attaattcta ccctatgaac atattccatt ttgtaatttc
     7561 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
     7621 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
     7681 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
     7741 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
     7801 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
     7861 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
     7921 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
     7981 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
     8041 gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt
     8101 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
     8161 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
     8221 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
     8281 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
     8341 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
     8401 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
     8461 ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc
     8521 ttcttggagg cttccagcgc ctcatctgga agtggaacac ctgtagcatc gatagcagca
     8581 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
     8641 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
     8701 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc
     8761 tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa
     8821 aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc
     8881 atgaacgctt ctctattcta tatgaaaagc cggttccggc gctctcacct ttcctttttc
     8941 tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa
     9001 tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc gttatgttga
     9061 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt
     9121 cccacagtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9181 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9241 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9301 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9361 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9421 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9481 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9541 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9601 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9661 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9721 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9781 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9841 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9901 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9961 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10021 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10081 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10141 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10201 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10261 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10321 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10381 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10441 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10501 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10561 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10621 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10681 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10741 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10801 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10861 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10921 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10981 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11041 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11101 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//
