LOCUS       sequence-337306-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of
            yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M
  TITLE     Records of RNA locations in living yeast revealed through covalent 
            marks.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 
            10.1073/pnas.1921408117. Epub 2020 Sep 9.
  PUBMED    32907940
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1921408117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-09-22- 22"; volume: "117"; issue: "38"; pages: 
            "23539-23547"
COMMENT     Sequence Label: pHCMM2
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             513..1226
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             513..1226
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(4061..4864)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      5186..5372
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5241..5265)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5380..5397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5380..5396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5389..5411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5833..7179
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(7317..8411)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8412..8817)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(8837..8855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8923..9027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9028..9096,9097..9888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9246..9265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10059..10647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10548..10567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10801..10818
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttag
      241 agctcggtgc tctcatgctt cttttctatt ttgagttatt aacgtcttag ctttataatg
      301 tcacaatctg acttcattac tgacacaatc gctcttataa cgcgaattgg tcactcgtta
      361 cggaaaggcg ttttctgaaa aatttgtctt gaacacgtcg aagatcactg aacgaataaa
      421 agatgcgact ggaacaatag tcagttataa ttgaccaaag aaggccttcg caacaaaata
      481 aatatactgc atattacagc acagcggccg caatgtctaa aggtgaagaa ttattcactg
      541 gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa ttttctgtct
      601 ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt atttgtacta
      661 ctggtaaatt gccagttcca tggccaacct tagtcactac tttaacttat ggtgttcaat
      721 gtttttctag atacccagat catatgaaac aacatgactt tttcaagtct gccatgccag
      781 aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac aagaccagag
      841 ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa ggtattgatt
      901 ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac tctcacaatg
      961 tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa attagacaca
     1021 acattgaaga tggttctgtt caattagctg accattatca acaaaatact ccaattggtg
     1081 atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc ttatccaaag
     1141 atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct gctggtatta
     1201 cccatggtat ggatgaattg tacaaaagac tagtaatggc tgatattact gataagacag
     1261 ctgaacaatt ggaaaacttg aatattcaag atgaccaaaa gcaagccgcc actggttcag
     1321 aaagccaatc tgttgaaaac tcttctgcat cattatatgt tggtgactta gaaccttctg
     1381 tttccgaagc ccacttatat gatatcttct ctccaatcgg ttcagtctcc tccattcgtg
     1441 tctgtcgtga tgccatcact aagacctctt tgggctatgc ttatgttaac tttaacgacc
     1501 atgaagccgg cagaaaagca attgagcaat tgaactacac tccaatcaag ggtagattat
     1561 gccgtattat gtggtctcaa cgtgacccat cattgagaaa gaagggttct ggtaacatct
     1621 ttatcaagaa cttgcaccct gatattgaca acaaggcttt gtatgacact ttctctgtgt
     1681 ttggtgacat cttgtccagc aagattgcca ccgacgaaaa cggaaaatcc aagggttttg
     1741 ggtttgttca cttcgaagaa gaaggtgctg ccaaggaagc tattgatgct ttgaatggta
     1801 tgctgttgaa cggtcaagaa atttatgttg ctcctcactt gtccagaaag gaacgtgact
     1861 ctcaattgga agagactaag gcacattaca ctaaccttta tgtgaaaaac atcaactccg
     1921 aaactactga cgaacaattc caagaattgt ttgccaaatt tggtccaatt gtttctgcct
     1981 ctttggaaaa ggatgctgat ggaaaattga agggtttcgg gtttgttaac tacgaaaagc
     2041 atgaagacgc tgtgaaagct gttgaagctt tgaatgactc tgaactaaat ggagaaaagt
     2101 tatacgttgg tcgtgcccaa aagaagaatg aacgtatgca tgtcttgaag aagcaatacg
     2161 aagcttacag attggaaaaa atggccaagt accaaggtgt taatttgttt gttaagaact
     2221 tagatgacag cgttgatgac gaaaagttgg aagaagaatt tgctccatat ggtactatca
     2281 cttctgcaaa ggttatgaga accgaaaacg gtaagtctaa gggttttggt tttgtttgtt
     2341 tctcaactcc agaggaagct actaaggcca ttacagaaaa gaaccaacaa attgttgctg
     2401 gtaagccatt atacgttgcc attgctcaaa gactagtcat gacagttatt cagaagtcgt
     2461 ctcctacagt gaaaggagtg aagacaacaa catcagattg caccgaaaga cgtcactcat
     2521 cgagttcacc ttgcaaaatg agtcaaaacg gaagcagtgg atactatgaa ggagatgttt
     2581 tactggatga tgatgctctt gtttcgatgc cagaagcaac gctcattgag tgctttggta
     2641 atctggctgt cacaaaaaga cctgcacatc tgagacctcc ggcagctttc tgtctaccgt
     2701 ttgtatcaat gaacaacgat ttgacgagcg gacatgcctt gaaacatatc acattgacat
     2761 cagtattcga cgcactacat ccgtacgttg ccatgccgta catcaacaga atggatatat
     2821 gctctgcaat gaacgtatat tggatcagaa actgtttgga agctgaacaa tccgatttat
     2881 ttcatcgatt cgctctggaa atgcaagtac atttgagtgc atgttttgga tgtcgcgttg
     2941 tcctggatat ctatggatca accagaaacg gattcggtac ccgattctgt gatgttgata
     3001 tgtcactttc tttttcaccg agtccacctt catgggcaac caattcggat cgagtgatga
     3061 gagctgttgc gaaggcactt gtcgattttc cgaaagcagt tgatgagaga tatgtcaacg
     3121 ccaaagttcc aattgtcaga ttcagaagca gtgacatgga tatggaagca gacatcagtt
     3181 acaagaatga tttggctctt cacaatactc agcttttgca acaatactgc aaatgggatc
     3241 ccgaaagact accgactctt ggagtttggg tcaaagcgtg ggcgaaacga agtggagttg
     3301 gagatgcatc aaaaggatcg ctgtcttcat atgcttggat tgtgatgctt attcattatc
     3361 ttcagcaagt cgaaccaatt cctgtgttgc catgtcttca ggaaatgaac catcagaaaa
     3421 gtgaaaatgt ctacgtccaa ggttacaaca cgtattattg gaaattcgta gacactgctc
     3481 gtacacgacg ttgtcgtgct tctgtcgttg atttgttcgt tggattcttg gattactatg
     3541 ccacatactt tgactactcc acaaatgtta tccagatggt gtccaagaaa ttggaattca
     3601 aaccggatcg ctggtgcaag tatccaatgt gtattgctga tccgttcgag acggatcata
     3661 atttggcaca aggtgttgat atgccaatgt ttgaatacat tagatcgtgt atggaacact
     3721 cgaagaaagt attcacggat cgccgcatgc gctcggagtt tctttcggga tacgggttcg
     3781 acgttgacga atttgatgca aggcatcgag gcgaaatgaa catggaaatg gcgtcacaat
     3841 ttggggaatt ccttcttcac aagtgcataa tggtgaaaca agccccgaat cgtcaattcc
     3901 gtgatcgcag tatgagtcaa tcgacgagca taagcaacac atcgagtatt tcctcgtcgg
     3961 gactgcatta agtaaccgca tagggtaata actgatataa ttaaattgaa gctctaattt
     4021 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc
     4081 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
     4141 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
     4201 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
     4261 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
     4321 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
     4381 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
     4441 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
     4501 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt
     4561 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg
     4621 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca
     4681 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct
     4741 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa
     4801 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg
     4861 acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg
     4921 ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct
     4981 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc
     5041 ggggtaccta actttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt
     5101 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag
     5161 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     5221 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     5281 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
     5341 tgaggtcgct cttattgacc acacctctac cgctcgagca ctggccgtcg ttttacaacg
     5401 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5461 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5521 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5581 acaccgcatt tatcggatcg tacttgttac ccatcattga attttgaaca tccgaacctg
     5641 ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat agtctagcgc
     5701 tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc tattgcatag
     5761 gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc acttcaatag
     5821 catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
     5881 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     5941 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
     6001 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     6061 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
     6121 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
     6181 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
     6241 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
     6301 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
     6361 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
     6421 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
     6481 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
     6541 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
     6601 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
     6661 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
     6721 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
     6781 tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac ttcggaatag
     6841 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     6901 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     6961 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     7021 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     7081 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     7141 ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat aagctgtcaa
     7201 acatgagaat taattctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta
     7261 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
     7321 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
     7381 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
     7441 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
     7501 gtggcgatag ggttgacctt attctttggc aaatctggag cagaaccgtg gcatggttcg
     7561 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
     7621 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
     7681 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
     7741 atcaattgat gttgaacctt caatgtagga aattcgttct tgatggtttc ctccacagtt
     7801 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
     7861 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
     7921 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
     7981 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
     8041 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
     8101 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactacct
     8161 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcagcctt cttggaggct
     8221 tccagcgcct catctggaag tggaacacct gtagcatcga tagcagcacc accaattaaa
     8281 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
     8341 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
     8401 ggggcagaca ttagaatggt atatccttga aatatatata tatattgctg aaatgtaaaa
     8461 ggtaagaaaa gttagaaagt aagacgattg ctaaccacct attggaaaaa acaataggtc
     8521 cttaaataat attgtcaact tcaagtattg tgatgcaagc atttagtcat gaacgcttct
     8581 ctattctata tgaaaagccg gttccggcgc tctcaccttt cctttttctc ccaatttttc
     8641 agttgaaaaa ggtatatgcg tcaggcgacc tctgaaatta acaaaaaatt tccagtcatc
     8701 gaatttgatt ctgtgcgata gcgcccctgt gtgttctcgt tatgttgagg aaaaaaataa
     8761 tggttgctaa gagattcgaa ctcttgcatc ttacgatacc tgagtattcc cacagttaat
     8821 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8881 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9241 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9541 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10681 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10741 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10801 agcgagtcag tgagcgagga agcggaaga
//
