LOCUS       sequence-337331-        7198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Brightbill HD, Plevy SE, Modlin RL, Smale ST
  TITLE     A prominent role for Sp1 during lipopolysaccharide-mediated 
            induction of the IL-10 promoter in macrophages.
  JOURNAL   J Immunol. 2000 Feb 15. 164(4):1940-51.
  PUBMED    10657644
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2000 Feb 15. 164(4):1940-51."
COMMENT     Sequence Label: pGL2B -1538/+64
FEATURES             Location/Qualifiers
     source          1..7198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1676..3328
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1742..1760)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3569..3634
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3764..3784
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4209..4343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4485..4502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4656..5244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4736..4755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5415..6206,6207..6275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6038..6057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6276..6380)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6407..6862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6494..6513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6704..6725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    7052..7173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagag ggtccatgct agctgcgtct
       61 tgagcctctt ctggggttca gatctctgat ctacagcagt gtgtccacac ctaaaacatc
      121 agctcagaga ggcagttgct tctgctgttg gaaacggaca tcccaaaaaa aaacaaaaaa
      181 cagaaatcaa aagggaagga gaaagtgaaa gggatggagg cagcttgtcc ccttccctgt
      241 gcttgctgct ggtagaaaac tcagcctgga actgaccgga gcagcagttc ttgagtcaat
      301 tccattccaa cttctagaag gttcttttcc cgtcgaagag tgacaggagg agaggccaga
      361 cccccttgat cctgatctgc cagccactgc atcagataag acgagataac cccgagttcc
      421 tgttctacca gccctggtgt ggtaaccctc tccaatgggg caggcttgga accctgtgcc
      481 aacgaagatc ctcccccgta ctgatgcagg aaggacagcc cgggagtgta ccctctacat
      541 gggtctactt ttatttaagc aaacattccc tggtcaacag gacgtgtagc attgcccccc
      601 ccccttgggt cacacagaaa acaggtacca ggaggacaag tagttgcttg cccagggtac
      661 agaatgaaag gcaatagggg actctaggcg aatgttcttc ccacccaaac tgaggtagta
      721 ggagaagtcc ctactgaagg gaaggtccag acataatcaa aggactacca gagatctccc
      781 aggtatctgt agaagtacta acatctccat ccttcaacag ctacaggtta cacgtctcca
      841 aggctgggac attgtaaaac agggccatgg taaggtctac ccgacagctc agagcaagcc
      901 tcccagaagt ctgagttcct tctcctaact tctcatgctg ggatctgagc ttcttcgtga
      961 aacacggggc agaggaggca ccagaactct cctctgacca actgccccac agcacacata
     1021 tcctcaaagg atagtcttga atacgtgatg gaagaattaa agagagtgag gtctgaagaa
     1081 aatcagccct ctcggggttt cctttgggta actgagtgct aaggtgactt ccgagtcagc
     1141 aagaaatatc ggacgttcaa cccaggttga gtggaggaaa caattatttc tcaatcctaa
     1201 tatgttctgg aatagcccat ttatccacgt cattatgacc tgggagtgcg tgaatggaat
     1261 ccacagatga gggcctctgt acatagaaca gctgtctgcc tcaggaaata caacttttag
     1321 tattgagagg ctaaaaagaa aaaaaaatta aaagagaggt agcccatact aaaaatagct
     1381 gtaatgcaga agttcattcc gaccagttct ttagcgctta caatgcaaaa aaaagggaaa
     1441 ggaaaaaaaa aaagaaagaa attaaactca aaaattgcat ggtttagaag agggaggagg
     1501 agcctgaata acaaaaacct ttgccaggaa ggccccactg agccttcagt ataaaagggg
     1561 gaccaagaac aggaggtcta catttagaga cttgctcttg cactaccaaa gccacaaggc
     1621 agccttgcag aaaagagagc tctatcaagc ttggcattcc ggtactgttg gtaaaatgga
     1681 agacgccaaa aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc
     1741 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     1801 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg
     1861 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     1921 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     1981 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca
     2041 gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     2101 attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca
     2161 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     2221 accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaattcct ctggatctac
     2281 tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc
     2341 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     2401 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     2461 cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat
     2521 tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga
     2581 caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga
     2641 agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct
     2701 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     2761 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     2821 gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt
     2881 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     2941 catagcttac tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat
     3001 taaatacaaa ggatatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc
     3061 caacatcttc gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc
     3121 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     3181 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     3241 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     3301 caagaagggc ggaaagtcca aattgtaaaa tgtaactgta ttcagcgatg acgaaattct
     3361 tagctattgt aatactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
     3421 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
     3481 agaaattcgc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     3541 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     3601 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     3661 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     3721 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     3781 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     3841 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     3901 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     3961 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     4021 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     4081 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     4141 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4201 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4321 atgtatctta tcatgtctgg atccgtcgac cgatgccctt gagagccttc aacccagtca
     4381 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4441 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     4501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5701 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6001 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     6421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6841 aaaatattaa cgcttacaat ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     6901 cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat
     6961 attaaggtac gtggaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     7021 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     7081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7141 ttgtggtttg tccaaactca tcaatgtatc ttatggtact gtaactgagc taacataa
//
