LOCUS       sequence-337334-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A control chimera; expresses C. elegans PUP-2 from the Sec63 
            promoter without the RNA-recognition motifs (RRMs) of yeast 
            poly(A)-binding protein [PUP alone (-PAB)].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M
  TITLE     Records of RNA locations in living yeast revealed through covalent 
            marks.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 
            10.1073/pnas.1921408117. Epub 2020 Sep 9.
  PUBMED    32907940
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1921408117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-09-22- 22"; volume: "117"; issue: "38"; pages: 
            "23539-23547"
COMMENT     Sequence Label: pHCMM3
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             513..1226
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             513..1226
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(2858..3661)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      3983..4169
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4038..4062)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4177..4194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4177..4193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4186..4208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4630..5976
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6114..7208)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7209..7614)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7634..7652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7720..7824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7825..7893,7894..8685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8043..8062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8856..9444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9345..9364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9598..9615
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttag
      241 agctcggtgc tctcatgctt cttttctatt ttgagttatt aacgtcttag ctttataatg
      301 tcacaatctg acttcattac tgacacaatc gctcttataa cgcgaattgg tcactcgtta
      361 cggaaaggcg ttttctgaaa aatttgtctt gaacacgtcg aagatcactg aacgaataaa
      421 agatgcgact ggaacaatag tcagttataa ttgaccaaag aaggccttcg caacaaaata
      481 aatatactgc atattacagc acagcggccg caatgtctaa aggtgaagaa ttattcactg
      541 gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa ttttctgtct
      601 ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt atttgtacta
      661 ctggtaaatt gccagttcca tggccaacct tagtcactac tttaacttat ggtgttcaat
      721 gtttttctag atacccagat catatgaaac aacatgactt tttcaagtct gccatgccag
      781 aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac aagaccagag
      841 ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa ggtattgatt
      901 ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac tctcacaatg
      961 tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa attagacaca
     1021 acattgaaga tggttctgtt caattagctg accattatca acaaaatact ccaattggtg
     1081 atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc ttatccaaag
     1141 atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct gctggtatta
     1201 cccatggtat ggatgaattg tacaaaagac tagtcatgac agttattcag aagtcgtctc
     1261 ctacagtgaa aggagtgaag acaacaacat cagattgcac cgaaagacgt cactcatcga
     1321 gttcaccttg caaaatgagt caaaacggaa gcagtggata ctatgaagga gatgttttac
     1381 tggatgatga tgctcttgtt tcgatgccag aagcaacgct cattgagtgc tttggtaatc
     1441 tggctgtcac aaaaagacct gcacatctga gacctccggc agctttctgt ctaccgtttg
     1501 tatcaatgaa caacgatttg acgagcggac atgccttgaa acatatcaca ttgacatcag
     1561 tattcgacgc actacatccg tacgttgcca tgccgtacat caacagaatg gatatatgct
     1621 ctgcaatgaa cgtatattgg atcagaaact gtttggaagc tgaacaatcc gatttatttc
     1681 atcgattcgc tctggaaatg caagtacatt tgagtgcatg ttttggatgt cgcgttgtcc
     1741 tggatatcta tggatcaacc agaaacggat tcggtacccg attctgtgat gttgatatgt
     1801 cactttcttt ttcaccgagt ccaccttcat gggcaaccaa ttcggatcga gtgatgagag
     1861 ctgttgcgaa ggcacttgtc gattttccga aagcagttga tgagagatat gtcaacgcca
     1921 aagttccaat tgtcagattc agaagcagtg acatggatat ggaagcagac atcagttaca
     1981 agaatgattt ggctcttcac aatactcagc ttttgcaaca atactgcaaa tgggatcccg
     2041 aaagactacc gactcttgga gtttgggtca aagcgtgggc gaaacgaagt ggagttggag
     2101 atgcatcaaa aggatcgctg tcttcatatg cttggattgt gatgcttatt cattatcttc
     2161 agcaagtcga accaattcct gtgttgccat gtcttcagga aatgaaccat cagaaaagtg
     2221 aaaatgtcta cgtccaaggt tacaacacgt attattggaa attcgtagac actgctcgta
     2281 cacgacgttg tcgtgcttct gtcgttgatt tgttcgttgg attcttggat tactatgcca
     2341 catactttga ctactccaca aatgttatcc agatggtgtc caagaaattg gaattcaaac
     2401 cggatcgctg gtgcaagtat ccaatgtgta ttgctgatcc gttcgagacg gatcataatt
     2461 tggcacaagg tgttgatatg ccaatgtttg aatacattag atcgtgtatg gaacactcga
     2521 agaaagtatt cacggatcgc cgcatgcgct cggagtttct ttcgggatac gggttcgacg
     2581 ttgacgaatt tgatgcaagg catcgaggcg aaatgaacat ggaaatggcg tcacaatttg
     2641 gggaattcct tcttcacaag tgcataatgg tgaaacaagc cccgaatcgt caattccgtg
     2701 atcgcagtat gagtcaatcg acgagcataa gcaacacatc gagtatttcc tcgtcgggac
     2761 tgcattaagt aaccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg
     2821 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc
     2881 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc
     2941 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat
     3001 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg
     3061 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc
     3121 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag
     3181 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg
     3241 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg
     3301 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga
     3361 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg
     3421 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat
     3481 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg
     3541 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata
     3601 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca
     3661 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc
     3721 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
     3781 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccggg
     3841 gtacctaact ttggacttct tcgccagagg tttggtcaag tctccaatca aggttgtcgg
     3901 cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata
     3961 cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     4021 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     4081 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga
     4141 ggtcgctctt attgaccaca cctctaccgc tcgagcactg gccgtcgttt tacaacgtcg
     4201 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4261 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4321 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4381 ccgcatttat cggatcgtac ttgttaccca tcattgaatt ttgaacatcc gaacctggga
     4441 gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt
     4501 acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta
     4561 atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat
     4621 atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     4681 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     4741 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     4801 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     4861 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     4921 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     4981 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     5041 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     5101 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     5161 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     5221 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     5281 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     5341 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     5401 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     5461 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     5521 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt
     5581 caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc ggaataggaa
     5641 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     5701 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     5761 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     5821 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     5881 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     5941 atccttcaat gctatcattt cctttgatat tggatcgatc cgatgataag ctgtcaaaca
     6001 tgagaattaa ttctacccta tgaacatatt ccattttgta atttcgtgtc gtttctatta
     6061 tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc tttttaagca
     6121 aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact gttggaacca
     6181 cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc ttcaatggcc
     6241 ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga caagatagtg
     6301 gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca tggttcgtac
     6361 aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg caacaaaccc
     6421 aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat gttgctggtg
     6481 attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc agaatcaatc
     6541 aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc cacagttttt
     6601 ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat aggcaatggt
     6661 ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac ttctggaacg
     6721 gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct cttaccaaag
     6781 taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc aaattgtggc
     6841 ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct taagttggcg
     6901 tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac actacctgta
     6961 ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt ggaggcttcc
     7021 agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc aattaaatga
     7081 ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag aaccttaatg
     7141 gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat cttcttaggg
     7201 gcagacatta gaatggtata tccttgaaat atatatatat attgctgaaa tgtaaaaggt
     7261 aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca ataggtcctt
     7321 aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta
     7381 ttctatatga aaagccggtt ccggcgctct cacctttcct ttttctccca atttttcagt
     7441 tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa
     7501 tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg
     7561 ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac agttaattct
     7621 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     7681 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8041 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8341 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9001 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9481 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9541 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9601 gagtcagtga gcgaggaagc ggaaga
//
