LOCUS       sequence-337336-        8226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 in Candida auris.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Santana DJ, O'Meara TR
  TITLE     Forward and reverse genetic dissection of morphogenesis identifies 
            filament-competent Candida auris strains.
  JOURNAL   Nat Commun. 2021 Dec 10;12(1):7197. doi: 10.1038/s41467-021-27545-5.
  PUBMED    34893621
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-10"; volume: "12(1):7197. doi"; pages: " 
            10.1038/s41467-021-27545-5"
COMMENT     Sequence Label: pTO135_pENO1-CaCas9-tCyc1
FEATURES             Location/Qualifiers
     source          1..8226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             222..4325
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             222..4325
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4338..4358
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4362..4382
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4386..4406
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4407..4472
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4553..4571)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4813..4829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4813..4829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4826..4848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4837..4853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4861..4867,4868..4885,4886..4891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4906..4927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5044..5061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5215..5803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5295..5314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5974..6765,6766..6834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6597..6616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6835..6939)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7007..7025
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7063..7085)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7185..7204
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7398..7420
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7412..7429
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7413..7429
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccctttgtgt gtgcaaataa tcatggtggc tccctaatgg cataccctca gtcacctgga
       61 ggaattgctc gctcgagtct cctgaggtgt cctcctgcct gtggaaaaag cggccaggtg
      121 tggtccaccg ttggaacagc ttgctgtata aataccacag gtgcgcacgc cccaggggcc
      181 tttttttttt ttctcctccc tatccaaact taattttcat catggataaa aagtatagta
      241 ttggtttaga tattggtact aactctgtgg gttgggcagt tatcactgac gaatataaag
      301 ttccatcaaa gaaatttaag gtgttaggta acactgacag acactcaata aaaaagaatc
      361 ttatcggtgc tcttttgttc gactccggtg aaactgccga ggctacacgt ttaaaaagaa
      421 cagcaagaag aagatatacc cgtagaaaaa atagaatatg ttatttacaa gaaatctttt
      481 ctaatgaaat ggctaaagtt gatgattcct ttttccatag attggaagag tcatttttgg
      541 ttgaagaaga caaaaagcat gagagacatc caatctttgg gaatatagtt gatgaagtgg
      601 cttaccatga aaaatatcct accatttatc atttaagaaa gaaattggta gattcaactg
      661 ataaagctga ccttagatta atctatttag cacttgccca tatgattaaa tttagaggtc
      721 attttttgat tgaaggtgat ttgaacccag ataattctga cgtggataaa ttatttattc
      781 aattagtcca aacctacaac caattatttg aggaaaatcc aattaatgct agtggtgtcg
      841 atgccaaagc tatattatca gccagattat caaaatctag acgtttggaa aatttgattg
      901 cccaattgcc aggagaaaaa aagaatggat tatttggaaa cttgatcgca ttatcattgg
      961 gtttgacacc aaattttaaa tctaattttg atttagctga agatgctaaa ttacaattat
     1021 caaaagacac ctatgacgac gatttggaca atttacttgc tcaaattggt gatcaatatg
     1081 cagatttgtt cttagctgct aaaaacttat ctgatgctat tttgttgtct gatattttga
     1141 gagtgaacac agaaataacc aaagctccat tatcagcatc tatgatcaaa cgttatgatg
     1201 aacaccatca ggatttgact ttattgaaag ctttggtgag acaacaattg ccagagaagt
     1261 ataaagaaat ctttttcgat caatctaaaa acgggtatgc aggttatatt gatgggggtg
     1321 cctcccaaga ggaattttac aaatttataa aacctatttt agaaaagatg gatgggactg
     1381 aggaactttt ggtcaaattg aacagagaag atttgttacg taaacagaga acttttgata
     1441 atggtagtat acctcaccaa attcatttgg gtgagttgca tgcaatttta agaagacaag
     1501 aagattttta tccattttta aaagataata gagaaaaaat cgagaaaatt ttaaccttta
     1561 gaattccata ctatgttggg cctttggcta gaggtaattc aagatttgcc tggatgacac
     1621 gtaaatcaga agaaactatt accccttgga attttgaaga ggttgttgat aaaggagcat
     1681 cagcacagag ttttattgaa agaatgacca atttcgataa aaacttacca aatgaaaaag
     1741 ttttaccaaa acattccttg ttatacgaat attttactgt ttacaatgaa cttacaaagg
     1801 ttaaatatgt tactgaaggt atgcgtaagc cagccttttt atctggagaa cagaaaaagg
     1861 caatagttga tttattgttt aaaacaaata gaaaagttac tgttaaacaa ttaaaagaag
     1921 attactttaa gaaaattgaa tgttttgatt cagttgaaat cagtggtgtt gaagacagat
     1981 ttaatgctag tttaggaact taccatgatt tacttaaaat tatcaaagat aaagatttct
     2041 tggataacga agaaaatgaa gacattttag aagacattgt tttaacctta actttattcg
     2101 aagatagaga gatgattgaa gaacgtttga agacttatgc acatttgttt gacgataaag
     2161 tgatgaaaca gttgaaaaga agacgttata ctggatgggg tagattgtct cgtaaattga
     2221 tcaatggaat tagagataaa caaagtggta aaactatctt ggactttttg aaatctgacg
     2281 gatttgctaa tagaaatttc atgcaattga tccacgacga tagtttgaca tttaaagaag
     2341 acatccaaaa ggcccaagtg agtgggcaag gtgattcatt acatgaacat attgcaaatt
     2401 tagccggatc tcctgctatt aagaaaggga tattacaaac tgttaaagtt gtggatgaat
     2461 tagtgaaagt aatgggaaga cataaacctg aaaacattgt cattgagatg gcaagagaaa
     2521 atcaaactac acaaaaagga cagaaaaata gtagagaacg tatgaaaaga atagaagagg
     2581 gtattaaaga attgggtagt caaatattga aagaacaccc agtggaaaat acccagttgc
     2641 aaaatgaaaa attatatctt tactaccttc aaaatggacg tgatatgtat gttgatcagg
     2701 aattagatat aaatagactt tcagattatg atgtagatca tatagttcca caatctttct
     2761 tgaaagatga ttccatagac aataaagtat taactagaag tgataaaaat agaggtaaaa
     2821 gtgataatgt cccaagtgag gaagtcgtca aaaagatgaa aaattactgg cgtcaacttt
     2881 tgaatgctaa attaattact caaagaaaat ttgataattt gactaaagca gaaagaggtg
     2941 ggctttctga attagataaa gccgggttca ttaaaagaca attggtcgaa actagacaaa
     3001 ttactaaaca tgttgcccaa attttagatt cccgtatgaa cactaagtat gacgaaaatg
     3061 ataagttaat acgtgaggtt aaagtcatta ctttaaaatc aaaacttgtc tctgatttca
     3121 gaaaggattt ccaattctat aaagttagag aaattaataa ttatcatcat gctcatgatg
     3181 catatttgaa tgctgtagtt ggaactgctt taatcaagaa ataccctaaa ttagaatctg
     3241 aatttgtata tggtgattac aaagtctatg atgttagaaa gatgattgct aaatcagaac
     3301 aagaaattgg taaagctaca gctaaatact tcttttactc taacattatg aatttcttta
     3361 aaacagaaat tactttggca aacggtgaaa ttagaaaaag acctcttatt gaaacaaatg
     3421 gtgagactgg agagatagtt tgggacaaag ggcgtgattt cgctactgtt agaaaagttt
     3481 tatcaatgcc acaagttaac attgtaaaga aaacagaggt tcaaactggt ggtttctcaa
     3541 aagaaagtat tttgcctaaa agaaatagtg ataaattaat tgccagaaaa aaggattggg
     3601 atccaaagaa atatggtggt ttcgactcac caaccgtagc ctattctgtt ttggttgtgg
     3661 caaaggttga aaagggtaaa agtaaaaagc ttaaatcagt aaaagaactt ttgggtatta
     3721 caataatgga aagaagttcc tttgaaaaga accctattga ttttttggaa gctaaaggtt
     3781 ataaggaagt aaagaaggac ttaataatca aattgcctaa atattcttta tttgaattag
     3841 aaaatgggag aaaaagaatg ttggcttctg ctggagaatt gcaaaagggt aatgaattag
     3901 cattgccttc caaatatgtt aacttcttgt atttagcttc acactatgaa aagttgaaag
     3961 ggtcaccaga agataacgag caaaaacaat tatttgttga acaacacaaa cactacttag
     4021 atgagattat agaacaaatt agtgaattca gtaaaagagt gatattagct gatgcaaatt
     4081 tagataaagt tttgtcagcc tataacaaac atagagataa gccaattaga gaacaagcag
     4141 aaaacattat tcacttattt acccttacca atttaggagc acctgctgct ttcaagtatt
     4201 ttgatacaac aattgatcgt aaaagatata cctcaacaaa agaagtctta gacgccacct
     4261 taattcatca atcaatcact ggattgtatg agacaagaat tgatttgtct caattgggtg
     4321 gtgatgaagg ggctgatcct aagaagaaaa gaaaagttga tccaaagaaa aagcgtaagg
     4381 tggatcctaa gaaaaagaga aaggttgact acaaagacca tgacggtgat tataaagatc
     4441 atgacatcga ctacaaggat gacgatgaca agtgataatg actgcagaga tccatcgacc
     4501 tgccgccaag ctaattccgg gcgaatttct gtcgagtcat gtaattagtt atgtcacgct
     4561 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
     4621 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
     4681 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
     4741 ccttgcttga gaaggttttg ggacggatcc tctagagtcg acctgcaggc atgcaagctt
     4801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5641 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6961 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7021 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7081 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7141 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     7201 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7261 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     7321 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7381 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct
     7441 cggtacccct ctttgtagtt caacttatgc tcactgctct tccctccaat accatctgtt
     7501 ctaccatctg ctctgcccta attaaactca ctcacctgtc gaacagcacc tgtcaaaaca
     7561 ggtgactcca ctaataatac aaatccacgt caaatgacgt gcacgaggga gtatgtcata
     7621 ttctatggcc gttagtctcc cggtcaaatt gaccaatcac aagacttacc tgaagacctg
     7681 actcaacgca atcgccagtc ccgggtttcc tggctcacgg gcccctccac tacgctgata
     7741 ttttgtcaat taaccaggag tctaccctag tgcgcattgt cgaagggagt ggaaagccag
     7801 ggaaaaaaaa cgtatagtgc ggtccccgtc gacccgagac gacaaggcac ccaggtgagt
     7861 caggctcaaa tgcatgaaaa attatgaaaa gtggaaaatc gatggccctc aggtgaaggt
     7921 tatcaaaacc tcatcagctc gtcgctcaat tgctaaaaat caactgaaac agcgtcccag
     7981 tggtgtcacc ccacggttgt ttcagggctg cggtggatca catggcgact ccctttggcc
     8041 cgtagggtgc gatccgtcag tgcccggggc cctctgccag gattctacgc gcattgcaac
     8101 tccgaggccg ataccctttg accagtggtc aattcgtcac aattggcaac attttcatga
     8161 tttggcagct tcacgttgaa caattgaacc agcagaatcc aggaaacgag tttccgttgt
     8221 gaatga
//
