LOCUS       sequence-33734-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pDE43-MCKq20
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(93..99,100..117,118..123))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(148..271)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        334..352
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     334..351
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(539..558)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(540..558)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(721..740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
       61 aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag cataaagtgt
      121 aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt tttacagtat
      181 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      241 gtttctcgtt caactttatt atacatagtt gcttaagctg caggggaccc tagaggtccc
      301 cttttttatt ttaaaaattt tttataaccg gtgatttagg tgacactata gaagtggaat
      361 tcgatggcct agctggcatc ggtacgtgcc gcgcaaccat gtagtagtcc tggagcgtgt
      421 ccatctggtg ttcaagcttg caacttcttc aagaggctgc cgtcccgcgc cgataccatg
      481 ctatagtatg gcgttcttgc tgctgatgga gcatgccata tggaaaccgt tgtggtctcc
      541 ctatagtgag tcgtattaat ttacgcgttt cactggccga catgtgagca aaaggccagc
      601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      781 cgcttaccgg atacctgtcc gcctttctcc c
//
