LOCUS       sequence-33735-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pDE43-MCSq22
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(96..102,103..120,121..126))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(151..274)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        337..355
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     337..354
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(542..561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(543..561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
       61 tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag
      121 tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag
      181 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      241 tacgtttctc gttcaacttt attatacata gttgcttaag ctgcagggga ccctagaggt
      301 cccctttttt attttaaaaa ttttttataa ccggtgattt aggtgacact atagaagtgg
      361 aattcgatgg cctagctggc atcggtacgt gccgcgcaac catgtagtag tcctggagcg
      421 tgtccatctg gtgttcaagc tttcgccgag cagtgcacgt actggacgtg tgacctgagc
      481 cgcgaataca tcgacatcaa cgcctgaagg ccggcatgcc atatggaaac cgttgtggtc
      541 tccctatagt gagtcgtatt aatttacgcg ttgcgctcgg tcgttcggct gcggcgagcg
      601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      781 aggtggcgaa acccgacagg actataaaga tacc
//
