LOCUS       sequence-33737-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pGMCKq26-10M-hyg
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..101)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccggtac
       61 cgaattcata acttcgtata gcatacatta tacgaagtta taggcctact gtcctcgttg
      121 atccttgccg agctgggatg gaagcccggc cgacccaccc tggaggagat gatcgaggat
      181 gccagggcct ttcacgcccg ccgctgctga gcgtccgccg ccgggcggcc cggggcgtca
      241 ggcgccgggg gcggtgtccg gcggccccca gaggaactgc gccagttcct ccggatcggt
      301 gaagccggag agatccagcg gggtctcctc gaacacctcg aagtcgtgca ggaaggtgaa
      361 ggcgagcagt tcgcgggcga agtcctcggt ccgcttccac tgcgccccgt cgagcagcgc
      421 ggccaggatc tcgcggtcgc cccggaaggc gttgagatgc agttgcacca ggctgtagcg
      481 ggagtctccc gcatagacgt cggtgaagtc gacgatcccg gtgacctcgg tcgcggccag
      541 gtccacgaag atgttggtcc cgtgcaggtc gccgtggacg aaccggggtt cgcggccggc
      601 cagcagcgtg tccacgtccg gcagccagtc ctccaggcgg tccagcagcc ggggcgagag
      661 gtagccccac ccgcggtggt cctcgacggt cgccgcgcgg cgttcccgca gcagttccgg
      721 gaagacctcg gaatgggggg tgagcacggt gttcccggcc agcggcaccc tgtgcagccg
      781 gccgagcacc cggccgagtt cgcgggcca
//
