LOCUS       sequence-33739-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pEN41A-T10M
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..117
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     170..186
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     171..187
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttaacgctac catggagctc caaataatga ttttattttg actgatagtg acctgttcgt
       61 tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataga aaagttgcat
      121 gaccaacttc gataacgttc tcggctcgat gatccccggg ggccccccct cgaggtcgac
      181 ggtatcgatt taattaatat gcatcaattg atttaaatac tagtgttaac tatttaattg
      241 gggaccctag aggtcccctt ttttatttta aaaatttttt caaggggacc tctagggtcc
      301 ccagctggct agcgagtcat gaggtcacca tggtcattag gagccgctct cgcacttcag
      361 ctgcttctcc aagccgcaga tgatcagctc caggccgaac aagaaggccg gctcggcgcc
      421 ctggtggtcg aacagctcga tggcctggcg cagcaacggc ggcatgctgt cggtggtcgg
      481 ggtctcgcgc tcctccttgg cgacctggtg ctcctggtcc tccaacacgc agcccagggt
      541 gaagtggccc acggccgaca gggcgtacaa cgcgttctcc agcgagaagc cctgctggca
      601 caggaaggcc aactggttct ccagggtctc gtactgcttc tcggtcggcc gggtgcccag
      661 gtgcaccttg gcgccgtcgc ggtggctcag caaggcgcag cggaagctct t
//
