LOCUS       sequence-337392-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag STK33
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3308..3332)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3391..3852
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3447..3464)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3674..3693
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3857..4651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3911..3930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4521..4540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4838..5307
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5343..5447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5448..5516,5517..6308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5666..5685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8074..8662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8563..8582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8816..8833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8937..9364)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9047..9068)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9259..9278
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatggct gatagtggct tagataaaaa atccacaaaa
     1801 tgccccgact gttcatctgc ttctcagaaa gatgtacttt gtgtatgttc cagcaaaaca
     1861 agggttcctc cagttttggt ggtggaaatg tcacagacat caagcattgg tagtgcagaa
     1921 tctttaattt cactggagag aaaaaaagaa aaaaatatca acagagatat aacctccagg
     1981 aaagatttgc cctcaagaac ctcaaatgta gagagaaaag catctcagca acaatggggt
     2041 cggggcaact ttacagaagg aaaagttcct cacataagga ttgagaatgg agctgctatt
     2101 gaggaaatct atacctttgg aagaatattg ggaaaaggga gctttggaat agtcattgaa
     2161 gctacagaca aggaaacaga aacgaagtgg gcaattaaaa aagtgaacaa agaaaaggct
     2221 ggaagctccg ctgtgaagtt acttgaacga gaggtgaaca ttctgaaaag tgtaaaacat
     2281 gaacacatca tacatctgga acaagtattt gaaacgccaa agaaaatgta ccttgtgatg
     2341 gagctttgtg aggatggaga actcaaagaa attctggata ggaaagggca tttctcagag
     2401 aatgagacaa ggtggatcat tcaaagtctc gcatcagcta tagcatatct tcacaataat
     2461 gatattgtac atagggatct gaaactggaa aatataatgg ttaaaagcag tcttattgat
     2521 gataacaatg aaataaactt aaacataaag gtgactgatt ttggcttagc ggtgaagaag
     2581 caaagtagga gtgaagccat gctgcaggcc acatgtggga ctcctatcta tatggcccct
     2641 gaagttatca gtgcccacga ctatagccag cagtgtgaca tttggagcat aggcgtcgta
     2701 atgtacatgt tattacgtgg agaaccaccc tttttggcaa gctcagaaga gaagcttttt
     2761 gagttaataa gaaaaggaga actacatttt gaaaatgcag tctggaattc cataagtgac
     2821 tgtgctaaaa gtgttttgaa acaacttatg aaagtagatc ctgctcacag aatcacagct
     2881 aaggaactac tagataacca gtggttaaca ggcaataaac tttcttcggt gagaccaacc
     2941 aatgtattag agatgatgaa ggaatggaaa aataacccag aaagtgttga ggaaaacaca
     3001 acagaagaga agaataagcc gtccactgaa gaaaagttga aaagttacca accctgggga
     3061 aatgtccctg agaccaatta cacttcagat gaagaggagg aaaaacagtc tactacttat
     3121 gaaaagcaat ttcctgcaac cagtaaggac aactttgata tgtgcagttc aagtttcaca
     3181 tctagcaaac tccttccagc tgaaatcaag ggagaaatgg agaaaacccc tgtgactcca
     3241 agccaaggaa cagcaaccaa gtaccctgct aaatccggcg ccctgtccag aaccaaaaag
     3301 aaactctacc caactttctt gtacaaagtg gtttgatcta gtcgaccaat tccggttatt
     3361 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3421 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3481 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3541 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3601 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3661 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3721 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3781 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3841 acacgatgat aataccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4021 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4381 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4621 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc atcgataaaa
     4681 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     4741 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     4801 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     4861 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     4921 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     4981 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     5041 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     5101 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     5161 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     5221 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     5281 tgactacccg tcagcggggg tctttcacag gccagtgggg ccgcactttt cggggaaatg
     5341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5461 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5521 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5581 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5641 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5701 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5761 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5821 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5881 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5941 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6001 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6061 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6121 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6181 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6241 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6301 attggtaact gtcagaccaa gtttactcat atatacttta gattgatttg cggccgcgaa
     6361 aaggacaagc agcgaaaatt cacgccccct tgggaggtgg cggcatatgc aaaggatagc
     6421 actcccactc tactactggg tatcatatgc tgactgtata tgcatgagga tagcatatgc
     6481 tacccggata cagattagga tagcatatac tacccagata tagattagga tagcatatgc
     6541 tacccagata tagattagga tagcctatgc tacccagata taaattagga tagcatatac
     6601 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
     6661 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc
     6721 catggcaaca ttagcccacc gtgctctcag cgacctcgtg aatatgagga ccaacaaccc
     6781 tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc
     6841 ccctatcttg gcccgcccac ctacttatgc aggtattccc cggggtgcca ttagtggttt
     6901 tgtgggcaag tggtttgacc gcagtggtta gcggggttac aatcagccaa gttattacac
     6961 ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc
     7021 cacaatttca aaaaaaagag tggccacttg tctttgttta tgggccccat tggcgtggag
     7081 ccccgtttaa ttttcggggg tgttagagac aaccagtgga gtccgctgct gtcggcgtcc
     7141 actctctttc cccttgttac aaatagagtg taacaacatg gttcacctgt cttggtccct
     7201 gcctgggaca catcttaata accccagtat catattgcac taggattatg tgttgcccat
     7261 agccataaat tcgtgtgaga tggacatcca gtctttacgg cttgtcccca ccccatggat
     7321 ttctattgtt aaagatattc agaatgtttc attcctacac tagtatttat tgcccaaggg
     7381 gtttgtgagg gttatattgg tgtcatagca caatgccacc actgaacccc ccgtccaaat
     7441 tttattctgg gggcgtcacc tgaaaccttg ttttcgagca cctcacatac accttactgt
     7501 tcacaactca gcagttattc tattagctaa acgaaggaga atgaagaagc aggcgaagat
     7561 tcaggagagt tcactgcccg ctccttgatc ttcagccact gcccttgtga ctaaaatggt
     7621 tcactaccct cgtggaatcc tgaccccatg taaataaaac cgtgacagct catggggtgg
     7681 gagatatcgc tgttccttag gaccctttta ctaaccctaa ttcgatagca tatgcttccc
     7741 gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact actacccggg
     7801 aagcatatgc tacccgttta gggttaacaa gggggcctta taaacactat tgctaatgcc
     7861 ctcttgaggg tccgcttatc ggtagctaca caggcccctc tgattgacgt tggtgtagcc
     7921 tcccgtagtc ttcctgggcc cctgggaggt acatgtcggc cgcaaaagga tctaggtgaa
     7981 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8221 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8701 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8761 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8821 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8881 gccgattcat taatgcaact atggttggcg cgttgtaaac gttaatattt tgttaaaatt
     8941 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa tagaccgaaa tcggcaaaat
     9001 cccttataaa tcaaaagaat agcccgagat agagttgagt gttgttccag tttggaacaa
     9061 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     9121 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
     9181 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     9241 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaagg cgctggcaag
     9301 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     9361 cgcgccgatc ttcaatattg gccattagcc atattattca ttggttatat agcataaatc
     9421 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
     9481 ggctcatgtc caatatgacc gccatgtt
//
