LOCUS       sequence-33740-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Blumenthal A, Trujillo C, Ehrt S, Schnappinger D
  TITLE     Simultaneous analysis of multiple Mycobacterium tuberculosis 
            knockdown mutants in vitro and in vivo.
  JOURNAL   PLoS One. 2010 Dec 22;5(12):e15667. doi: 
            10.1371/journal.pone.0015667.
  PUBMED    21203517
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-22"; volume: "5(12):e15667. doi"; pages: " 
            10.1371/journal.pone.0015667"
COMMENT     Sequence Label: pEN41A-T10M
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(170..274)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(371..495)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
       61 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
      121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
      181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
      301 acatcgcggc cgccctgcag ctctagagct cgaattctac aggtcactaa taccatctaa
      361 gtagttggtt catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt
      421 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt
      481 tttgtacaaa cttgtcagat ctcgtaatat tggatcgtcg caccgggtta agcctggagt
      541 gcggtggtgc ctggtcggca ttttcgcagt cgagggctct cgtgtagcct gggcgagttg
      601 ccgacgcagg cgaccctcct gccacggatc cggtaccatc aggaggaatc acttcgcaat
      661 gtcccgcctg gacaagtcca aggtcatcaa ctccgccctg gagctgctg
//
